Algal Crude Fucoidan Alone or with Bacillus subtilis DSM 17299 in Broiler Chickens Diet: Growth Performance, Carcass Characteristics, Blood Metabolites, and Morphology of Intestine

Document Type: Original Paper


Department of Animal and Poultry Nutrition, Faculty of Animal Science,Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran


This study was conducted to evaluate the effects of algal fucoidan and probiotic Bacillus subtilison growth performance, blood metabolites and intestinal morphology in broiler chickens. A total of 250 one-day-old Ross 360 male broiler chickens were randomly distributed into 5 treatments (6 replication pens/treatment) and reared for 42 d. The 5 dietary treatments were as follow: 1) a corn-soybean meal basal diet (control treatment); 2) a basal diet supplemented with antibiotic oxytetracycline; 3) a basal diet supplemented with the prebiotic fucoidan; 4) a basal diet supplemented with a probiotic product containing Bacillus subtilis spores; and 5) a basal diet supplemented with both the prebiotic and probiotic (served as a synbiotic). Birds received antibiotic and probiotic exhibited greater body weight gain (about 5.42% and 4.80%, respectively) than control treatment. The percentage of thigh and abdominal fat in birds fed probiotic diet were lower compared to the antibiotic treatment. The use of fucoidan and probiotic resulted lower (P< 0.05) serum concentration of triglyceride than those of antibiotic treatment. Supplementing of synbiotic increased villus height and villus height to crypt depth ratio in the jejunum (P< 0.05). Present study revealed that supplementing of probiotics with fucoidan could be advised as an effective synbiotic, instead of antibiotics, to improve the performance and health of broiler chickens.


Abramoff MD, Magalhães PJ & Ram SJ. 2004. Image processing with ImageJ. Biophotonics International, 11:36-42.

Al-Khalaifah HS. 2018. Benefits of probiotics and/or prebiotics for antibiotic-reduced poultry. Poultry Science, 97:3807-3815.DOI: 10.3382/ps/pey160

Alkhalf A, Alhaj M, & Al-homidan I.2010. Influence of probiotic supplementation on blood parameters and growth performance in broiler chickens.Saudi Journal of Biological Sciences, 17:219-225.DOI: 10.1016/j.sjbs.2010.04.005

Allahdo P, Ghodraty J, Zarghi H, Saadatfar Z, Kermanshahi H & Edalatian Dovom MR. 2018. Effect of probiotic and vinegar on growth performance, meat yields, immune responses, and small intestine morphology of broiler chickens.Italian Journal of Animal Science, 17:675-685. DOI: 10.1080/1828051X.2018.1424570

Ashayerizadeh O, Dastar B, Samadi F, Khomeiri M, Yamchi A & Zerehdaran S. 2017. Study on the chemical and microbial composition and probiotic characteristics of dominant lactic acid bacteria in fermented poultry slaughterhouse waste.Waste Management, 65:178-185. DOI: 10.1016/j.wasman.2017.04.017

Aviagen. 2014. Ross 308 Broiler: Nutrition Specifications. Aviagen Limited, Newbridge, Midlothian EH28 8SZ, Scotland, UK.

Awad WA, Ghareeb K, Abdel-Raheem S & Böhm J. 2009. Effects of dietary inclusion of probiotic and synbiotic on growth performance, organ weights, and intestinal histomorphology of broiler chickens.PoultryScience, 88:49-56. DOI: 10.3382/ps.2008-00244

Busher JT. 1990. Serum albumin and globulin. In: Walker HK, Hall WD, Hurst JW (eds) Clinical methods: The history, physical, and laboratory examinations. Butterworths, a division of Reed Publishing., Boston, pp 497-499.

FAO/WHO. 2001.Health and nutritional properties of probiotics in food including powder milk with live lactic acid bacteria, American Córdoba Park Hotel, Córdoba, Argentina.

Flórez-Fernández N, Torres MD, González-Muñoz MJ & Domínguez H. 2018. Potential of intensification techniques for the extraction and depolymerization of fucoidan. Algal Research, 30:128-148. DOI: 10.1016/j.algal.2018.01.002

Gibson GR, Hutkins R, Sanders ME, Prescott SL, Reimer RA, Salminen SJ, Scott K, Stanton C, Swanson KS, Cani PD, Verbeke K & Reid G. 2017. Expert consensus document: The international scientific association for probiotics and prebiotics (ISAPP) consensus statement on the definition and scope of prebiotics.Nature reviews. Gastroenterology &Hepatology, 14:491-502. DOI: 10.1038/nrgastro.2017.75

Gratz SW, Mykkanen H&El-Nezami HS. 2010. Probiotics and gut health: A special focus on liver diseases. World Journal of Gastroenterol, 16: 403-410. DOI: 10.3748/wjg.v16.i4.403

Guetiya Wadoum RE,Zambou NF, Anyangwe FF, Njimou JR, Coman MM, Verdenelli MC, Cecchini C, Silvi S, Orpianesi C, Cresci A & Colizzi V. 2016. Abusive use of antibiotics in poultry farming in Cameroon and the public health implications.British Poultry Science, 57:483-493. DOI: 10.1080/00071668.2016.1180668

Haldar S, Ghosh TK, Toshiwati & Bedford MR. 2011. Effects of yeast (Saccharomyces cerevisiae) and yeast protein concentrate on production performance of broiler chickens exposed to heat stress and challenged with Salmonella enteritidis.Animal Feed Science and Technology, 168:61-71. DOI: 10.1016/j.anifeedsci.2011.03.007

Heim G, Sweeney T, O'Shea CJ, Doyle DN & O’Doherty JV. 2015. Effect of maternal dietary supplementation of laminarin and fucoidan, independently or in combination, on pig growth performance and aspects of intestinal health.Animal Feed Science and Technology, 204:28-41. DOI: 10.1016/j.anifeedsci.2015.02.007

Huyghebaert G & Pack M. 1996. Effects of dietary protein content, addition of nonessential amino acids and dietary methionine to cysteine balance on responses to dietary sulphur‐containing amino acids in broilers.British poultry science, 37:623-639. DOI: 10.1080/00071669608417892

Khatibjoo A, Mahmoodi M, Fattahnia F, Akbari-Gharaei M, Shokri AN & Soltani S. 2018. Effects of dietary short- and medium-chain fatty acids on performance, carcass traits, jejunum morphology, and serum parameters of broiler chickens.Journal of Applied Animal Research, 46:492-498. DOI: 10.1080/09712119.2017.1345741

Lim SJ, Wan Aida WM, Schiehser S, Rosenau T& Böhmdorfer S. 2019. Structural elucidation of fucoidan from Cladosiphon okamuranus (Okinawa mozuku). Food Chemistry, 272:222-226. DOI: 10.1016/j.foodchem.2018.08.034

Liong MT, Dunshea FR & Shah NP. 2007. Effects of a synbiotic containing Lactobacillus acidophilus ATCC 4962 on plasma lipid profiles and morphology of erythrocytes in hypercholesterolaemic pigs on high- and low-fat diets. British Journal of Nutrition, 98: 736–744. DOI: 10.1017/S0007114507747803

Manafi M, Khalaji S, Hedayati M & Pirany N. 2017. Efficacy of Bacillus subtilis and bacitracin methylene disalicylate on growth performance, digestibility, blood metabolites, immunity, and intestinal microbiota after intramuscular inoculation with Escherichia coli in broilers. Poultry Science, 96:1174-1183. DOI: 10.3382/ps/pew347

Mehdi Y,Létourneau-Montminy M-P, Gaucher M-L, Chorfi Y, Suresh G, Rouissi T, Brar SK, Côté C, Ramirez AA & Godbout S. 2018. Use of antibiotics in broiler production: Global impacts and alternatives. Animal Nutrition, 4:170-178. DOI: 10.1016/j.aninu.2018.03.002

O'Sullivan L, Murphy B, McLoughlin P, Duggan P, Lawlor PG, Hughes H&Gardiner GE. 2010. Prebiotics from marine macroalgae for human and animal health applications. Marine Drugs, 8:2038-2064. DOI: 10.3390/md8072038

Palanisamy S, Vinosha M, Marudhupandi T, Rajasekar P & Prabhu NM. 2017.In vitro antioxidant and antibacterial activity of sulfated polysaccharides isolated from Spatoglossum asperum.Carbohydrate Polymers, 170:296-304. DOI: 10.1016/j.carbpol.2017.04.085

Park J, Yeom M & Hahm DH. 2016. Fucoidan improves serum lipid levels and atherosclerosis through hepatic SREBP-2-mediated regulation. Journal of Pharmacological Sciences, 131:84-92. DOI: 10.1016/j.jphs.2016.03.007

Pereira DIA & Gibson GR. 2002. Cholesterol assimilation by lactic acid bacteria and bifidobacteria isolated from the human gut. Applied and Environmental Microbiology, 68:4689-4693. DOI: 10.1128/AEM.68.9.4689-4693.2002

Potten CS. 1998. Stem cells in gastrointestinal epithelium: numbers, characteristics and death.Philosophical Transactions of the Royal Society of London. Series B: Biological Sciences, , 353:821-830. DOI: 10.1098/rstb.1998.0246

Ribeiro A, Vogt L, Canal C, Cardoso M, Labres R, Streck A, & Bessa M. 2007. Effects of prebiotics and probiotics on the colonization and immune response of broiler chickens challenged with Salmonella enteritidis.Brazilian Journal of Poultry Science, 9:193-200. DOI: 10.1590/S1516-635X2007000300009

SAS (2009) User’s Guide: Statistics, Version 9.2. SAS Inst, Inc., Cary, NC, US

Sohail MU,Ijaz A, Younus M, Shabbir MZ, Kamran Z, Ahmad S, Anwar H, Yousaf MS, Ashraf K, Shahzad AH & Rehman H. 2013. Effect of supplementation of mannan oligosaccharide and probiotic on growth performance, relative weights of viscera, and population of selected intestinal bacteria in cyclic heat-stressed broilers.The Journal of Applied Poultry Research, 22:485-491. DOI: 10.3382/japr.2012-00682

Sweeney T, Meredith H, Vigors S, McDonnell MJ, Ryan M, Thornton K & O’Doherty JV. 2017. Extracts of laminarin and laminarin/fucoidan from the marine macroalgal species Laminariadigitata improved growth rate and intestinal structure in young chicks, but does not influence Campylobacter jejuni colonisation.Animal Feed Science and Technology, 232:71-79. DOI: 10.1016/j.anifeedsci.2017.08.001

Tavaniello S, Maiorano G, Stadnicka K, Mucci R, Bogucka J & Bednarczyk M. 2018. Prebiotics offered to broiler chicken exert positive effect on meat quality traits irrespective of delivery route. PoultryScience, 97:2979-2987. DOI: 10.3382/ps/pey149

Walsh AM, Sweeney T, O'Shea CJ, Doyle DN  & Doherty JVO. 2013. Effect of supplementing varying inclusion levels of laminarin and fucoidan on growth performance, digestibility of diet components, selected faecal microbial populations and volatile fatty acid concentrations in weaned pigs.Animal Feed Science and Technology, 183:151-159. DOI: 10.1016/j.anifeedsci.2013.04.013

Wang CY, Wu TC, Hsieh SL, Tsai YH, Yeh CW & Huang CY. 2015. Antioxidant activity and growth inhibition of human colon cancer cells by crude and purified fucoidan preparations extracted from Sargassum cristaefolium.Journal of Food and Drug Analysis, 23:766-777. DOI: 10.1016/j.jfda.2015.07.002

Wang S, Peng Q, Jia HM, Zeng XF, Zhu JL, Hou CL, Liu XT, Yang FJ, Qiao SY. 2017. Prevention of Escherichia coli infection in broiler chickens with Lactobacillus plantarum B1. PoultryScience, 96:2576-2586. DOI: 10.3382/ps/pex061

Wang X, Kiess AS, Peebles ED, Wamsley KGS & Zhai W. 2018. Effects of Bacillus subtilis and zinc on the growth performance, internal organ development, and intestinal morphology of male broilers with or without subclinical coccidia challenge. PoultryScience, 97:3947-3956. DOI: 10.3382/ps/pey262

Yang Q,Ren S, Niu T, Guo Y, Qi S, Han X, Liu D & Pan F. 2014. Distribution of antibiotic-resistant bacteria in chicken manure and manure-fertilized vegetables.Environmental Science and Pollution Research International, 21:1231-1241. DOI: 10.1007/s11356-013-1994-1

Zaghari M, Zahroojian N, Riahi M & Parhizkar S. 2015. Effect of bacillus subtilis spore (Gallipro®) nutrients equivalency value on broiler chicken performance. Italian Journal of Animal Science, 14:3555. DOI: 10.4081/ijas.2015.3555

Zentek Jr,Hall EJ, German A, Haverson K, Bailey M, Rolfe V, Butterwick R& Day MJ. 2002. Morphology and immunopathology of the small and large intestine in dogs with nonspecific dietary sensitivity. The Journal of Nutrition, 132:1652S-1654S. DOI: 10.1093/jn/132.6.1652S.