Effect of Rolled or Extruded Flaxseeds in Finisher Diet on Pellet Quality, Performance, and n-3 Fatty Acids in Breast and Thigh Muscles of Broiler Chickens

Document Type: Original Paper


Department of Animal Science, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran


An experiment was conducted to compare the effect of corn-soybean meal finisher (29-42d) diets containing flaxseeds (rolled or extruded) on pellet quality, performance, n-3 fatty acids (FA) and oxidative stability of meat in broiler chickens. Seven pelleted diets were provided in a 3×2 factorial arrangement with three flaxseed levels (5, 10, and 15%) and two processing methods (rolled or extruded) and a zero flaxseed diet (control). Birds fed diet containing 15% flaxseed had significantly lower weight gain and higher feed conversion ratio compared to those fed diets contained 0, 5 and/or 10% flaxseed. The type of flaxseed processing did not influence (P > 0.05) fatty acid profile and the MDA content in muscles. Chickens fed the control diet had relatively high levels of saturated and monounsaturated fatty acids and low levels of polyunsaturated fatty acids. Feeding diets contained flaxseed markedly reduced the levels of saturated fatty acids, monounsaturated fatty acids and increased polyunsaturated fatty acids, particularly the n-3 fatty acids in meat. It is concluded that the addition of 15% flaxseeds to finisher diet can increase n-3 fatty acids and lipid peroxidation in meat, while reducing growth performance of broiler chickens. However, feeding finisher diet containing 10% flaxseed compared to 5 or 15% flaxseed is of more practical to achieve an acceptable level of Omega-3 fatty acids in breast and thigh muscles without compromising the performance of broiler chickens.


Abdollahi MR, Ravindran V, Weater TJ, Ravindran G & Thomas DV. 2010. Influence of conditioning temperature on the performance, nutrient utilization and digestive tract development of broilers fed on maize- and wheat-based diets. British Poultry Science, 51: 648–657. DOI: 10.1080/00071668.2010.522557

Agarwal R & Chase S D. 2002. Rapid, fluorimetric-liquid chromatographic determination of malondialdehyde in biological samples. Journal of Chromatography, B: 775, 121-126. DOI: 10.1016/S1570-0232(02)00273-8

Ajuyah AO, Ahn DU, Hardin RT & Sim JS. 1993. Dietary anti-oxidants and storage affect chemical characteristics of w-3 fatty acid-enriched broiler chicken meats. Journal of Food Science, 61: 43-46.  DOI: 10.1111/j.1365-2621.1993.tb03206.x

Ajuyah AO, Lee KH & Sim JS. 1990. Effect of feeding fuIl-fat canola and flax seed on broiler white and dark meats. Journal of Poultry Science, 69:1851-1859. DOI: 10.1111/j.1365-2621.1993.tb03206.x

Ajuyah AO, Lee KH, Hardin RT & Sim JS. 1991. Changes in the yield and in the fatty acid com position of whole carcass and selected meat portions of broiler chickens fed full-fat oil seeds. Poultry Science, 70: 2304-2314. DOI: 10.3382/ps.0702304

Alzueta C, Rodriguez MI, Cutuli MT, Rebole A, Ortiz LT, Centeno C & Trevino J. 2003. Effect of

whole and demucilaged linseed in broiler chicken diets on digesta viscosity, nutrient utilization and intestinal microflora. British Poultry Science, 44: 67–74. DOI: 10.1080/0007166031000085337.

Anjum FM, Haider MF, Khan MI, Sohaib S & Arshad MS. 2013. Impact of extruded flaxseed meal supplemented diet on growth performance, oxidative stability and quality of broilermeat and meat products. Lipids in Health and Disease. 12: 13-25.DOI: 10.1186/1476-511X-12-13

AOAC. 2005. Official Methods of Analysis, 18th ed. AOAC International, Washington DC.

Aviagen. 2014. ROSS 308 broilers nutrition specifications.

Baeza E, Chartrin P, Lessire M, Mateau K, Chesneau G, Guillevic M &Mourot J. 2015. Is it possible to increase n-3 fatty acid content of meat without affecting its technological and/or sensory quality and the growing performance of chickens? British Poultry Science, 56: 543– 550. DOI: 10.1080/00071668.2015.1068428

Bedford MR. 1995. Mechanism of action and potential environmental benefits from the use of feed enzymes. Animal Feed Science and Technology, 53: 145–155.  DOI: 10.1016/0377-8401(95)02018-U

Betti M, Perez TI, Zuidhof MJ &Renema RA. 2009a. Omega-3-enriched broiler meat: 3. Fatty acid distribution between triacylglycerol and phospholipids classes. Poultry Science, 88: 1740-1754. DOI: 10.3382/ps.2008-00449

Betti M, Schneider BL, Wismer WV & Carney VL. 2009b. Omega-3enriched broiler meat: 2. Functional properties, oxidative stability, and consumer acceptance. Poultry Science, 88:1085–1095. DOI: 10.3382/ps.2008-00158.

Bhatty RS. 1993. Further compositional analyses of flax: Muvilage, trypsin inhibitors and hydrocyanic acid. Journal of American Oil Chemists Society, Society, 70: 899-904. DOI: 10.1007/BF02545351.

Bond JM, Julian RJ &Scuires EJ. 1997. Effect of dietary flaxseed on broiler growth, erythrocyte deformability, and fatty acid composition of erythrocyte membranes. Canadian Journalof Animal Science,77: 279–286. DOI: 10.41411/A96-068

Chartrin P, Berri, C, Lebihan-Duval E, Quentin M, &Bae´za E. 2005. Lipid and fatty acid composition of fresh and cured–cooked breast meat of standard, certified and label chickens. Archives Geflugelkunst, 69, 219–225.

Choct M, Hughes RJ, Wang J, Bedford MR, Morgan AJ &Annison G. 1996. Increased small intestinal fermentation is partly responsible for the anti-nutritive activity of non-starch polysaccharides in chickens. British Poultry Science, 37: 609–621. DOI: 10.1080/00071669608417891.

Corzo A &Loar RE. 2011. Effects of feed formulation on feed manufacturing and pellet quality characteristics of poultry diets. World's Poultry Science, 67: 19-28. DOI:  10.1017/S004393391100002X

Cunnane SC, Botsoglou NA, FletourisDJ, PapageorgiouGE, VassilopoulosVN, Mantis AJ &Trakatellis A G. 1994. Rapid, sensitive, and specific thiobarbituric acid method for measuring lipid peroxidation in animal tissue, food, and feedstuff samples. Journal of Agricultural and Food Chemistry, 42: 1931–1937. DOI: 10.1093/ajcn/61.1.62

Cunnane SC, Hamadeh MJ, Liede AC, Thompson LU, Wolever TM & Jenkins DJ. 1995. Nutritional attributes of traditional flaxseed in healthy young adults. American Journal of Clinical Nutrition, 61: 62– 68. DOI: 10.1093/ajcn/61.1.62

Edney MJ, Campbell GL & Classen HL. 1989. The effect of b -glucanase supplementation on nutrient digestibility and growth in broilers given diets containing barley, oat groats or wheat. Animal Feed Science and Technology, 25: 193–200. DOI: 10.1016/0377-8404(89)90119-3

Fletcher DL. 1999. Broiler breast meat color variation, pH, and texture.  Poultry Science, 79: 1323–1327. DOI: 10.1093/ps/78.9.1323

Folch J, Less M & Stanley G HS. 1957. A simple method for the isolation and purification of total lipids from animal tissues.  Journal of Biological Chemistry, 226: 497–509.

Gonzalez-Esquerra R & Leeson S. 2000. Effects of menhaden oil and flaxseed in broiler diets on sensory quality and lipid composition of poultry meat. British Poultry Science, 41:481–488. DOI: 10.1080/713654967

Grau A, Guardiola F, Grimpa S, Barroeta A C &Codony R. 2001. Oxidative stability of dark chicken meat through frozen storage: Influence of dietary α-tocopherol and ascorbic acid supplementation.  Poultry Science, 80: 1630–1642. DOI: 10.1093/ps/80.11.1630

Honikel KO. 1998. Reference methods for the assessment of physical characteristics of meat. Meat Science, 49: 447–457. DOI: 10.1016/S0309-1740(98)00034-5

Hulan HW, Proudfoot FG, Ackman RG & Ratnayake WMN. 1988. omega-3 fatty acid levels and performance of broiler chickens fed redfish meal or redfish oil. Canadian Journal of Animal Science, 68: 533-547. DOI: 10.4141/cjas88-059

Hulan HW, Proudfoot PG & Nash DM. 1984. The effects of different fat sources on general performance and carcass fatty acid composition. Poultry Science, 63: 324–332. DOI: 10.3382/ps0630324

Jia W, Slominski BA, Bruce HL, Blank G, Nyachoti
CM & Jones RO. 2009. Enzyme addition facilitates the post-disease compensatory growth of broiler chickens challenged with Clostridium perfringens. Canadian Journal Animal Science, 89: 369-381. DOI: 10.4141/CJAS09017

Kamran-Azad S, Rahimi Sh, & Karimi-Torshizi MA. 2009. Effect of dietary oil seeds on n-3 fatty acid enrichment, performance parameters and humoral immune response of broiler chickens. Iranian Journal of Veterinary Research, 10: 158-165.  DOI: 10.22099/IJVR.2009.1669

Lee KH, Olomu JM & Sim JS. 1991. Live performance, carcass yield, protein and energy retention of broiler chickens fed canola and flax full-fat seeds and the restored mixtures of meal and oil. Canadian Journal Animal Science, 71: 897-903. DOI: 10.4141/cjas91-105

Lee K, Qi GH & Sim JS. 1995. Metabolizable energy and amino acid availability of full-fat seeds, meals and oils of flax and canola. Poultry Science, 74: 1341–1348. DOI: 10.3382/ps.0741341

Longstaff M &McNab JM. 1991. The inhibitory effects of hull polysaccharides and tannins of field beans (Viciafaba L.) on the digestion of amino acids, starch and lipid and on digestive enzyme activities in young chicks. British Journal of Nutrition, 65: 199-216. DOI: 10.1079/BJN19910081

Lopez-Ferrer S, Baucellas MD, Barroeta AC, Galobart J &Grashorn MA. 2001. n-3 enrichment of chicken meat. 2. Use of precursors of long-chain polyunsaturated fatty acids: Linseed oil.  Poultry Science, 80: 753–761. DOI: 10.1093/ps/80.6.753

Madhusudhan KT, Ramesh JP, Ogawua T, Sasaka K & Singh N. 1986. Detoxification of commercial linseed meal for use in broiler rations. Poultry Science, 65: 164–171. DOI: 10.3382/ps.0650164

Metcalf LD, Schmitz AA & Pelka JR. 1966. Rapid Preparation of Fatty Acid Esters from Lipids for Gas Chromatographic Analysis. Analytical Chemistry, 38: 514–515. DOI: 10.1021/ac60235a044

Morrissey PA, Sheehy PJA, Galvin K, Kerry JP & Buckley DJ. 1998. Lipid stability in meat and meat products. Meat Science, 49: S73-S86. DOI: 10.1016/S0309-1740(98)90039-0

Mridula D, Kaur D, Nagra SS, Barnwal P, Gurumayum S & Singh KK. 2011. Growth performance, carcass traits and meat quality in broilers, fed flaxseed meal. Asian-Australasian Journal of Animal Sciences, 24: 1729-1735. DOI: 10.5713/ajas.2011.11141

Nam K, Lee H, Joo Y, Kim K & Kang CH. 1998. Influence of builder’s sand on the TME of linseed for poultry. Animal Feed Science and Technology, 72: 199–201. DOI: 10.1016/S0377-8401(97)00176-4

Olomu JM &Baracos VE. 1991. Influence of dietary flaxseed oil on the performance, muscle protein deposition, and fatty acid composition of broiler chicks. Poultry Science, 70: 1403–1411. DOI: 10.3382/ps.0701403

Pohja NS &Niinivaara FP. 1957. Die bestimmung der wasserbindung des fleischesmittels der konstantdruckmethode. Fleischwirtschaft, 9: 193-195.

Rodriguez ML, Alzueta C, Rebole A, Ortiz LT, Centeno C & Trevin J. 2001. Effect of Inclusion Level of Linseed on the Nutrient Utilization of Diets for Growing Broiler Chickens. British Poultry Science, 42: 368–375. DOI: 10.1080/00071660120055359

Rodriguez-Leyva D, Dupasquier CM, McCullough R & Pierce GN. 2010. The cardiovascular effects of flaxseed and its omega_3 fatty acid, alpha_linolenic acid. Canadian Journal of Cardiology, 26: 489-496. DOI: 10.1016/S0828-282X(10)70455-4

Roth-Maier DA, Eder K &Kirchgessner M. 1998. Live performance and fatty acid composition of meat in broiler chickens fed diets with various amount of ground or whole flaxseed. Journal of Animal Physiology Animal Nutrition, 79: 260–268. DOI: 10.1111/j.1439-0396.1998.tb00649.x

Rymer C & Givens DI. 2005. N-3 fatty acid enrichment of edible tissue of poultry. Journal of Lipids, 40: 121-30. DOI: 10.1007/s11745-005-1366-4

Salmon RE &O’neil JB. 1973. The Effect of the Level and Source and of a Change of Source of Dietary Fat on the Fatty Acid Composition of the Depot Fat and the Thigh and Breast Meat of Turkeys as Related to Age. Poultry Science, 52: 302–314. DOI:  10.3382/ps.0520302

SAS (Statistical Analysis System). 2004. SAS/STAT. User’s guide: statistics SAS institute Inc, Cary, NC, USA.

Shen Y, Feng D, Fan Ming Z & Chavez E R. 2005. Performance, carcass cut-up and fatty acids deposition in broilers fed different levels of pellet-processed flaxseed. Journal of the Science of Food and Agriculture, 85: 2005-2014. DOI: 10.1002/jsfa.2155

Smits CHM, Veldman A, Verstegen MWA &Beynen AC. 1997. Dietary carboxymethylcellulose with high instead of low viscosity reduces macronutrient digestion in broiler chickens.  Journal of Nutrition, 127: 483–487. DOI: 10.1093/jn/127.3.483

Thacker PA, Campbell GL & Xu Y. 1994. Composition and nutritive value of acidulated fatty acids, degummed canola oils and tallow as energy sources for starting broiler chicks. Animal Feed Science, Technology, 46: 251–260. DOI: 10.1016/0377-8401(94)90143-0

Wu M, Li D, Wang L, Zhou Y, Brooks MS & Chen XD. 2008. Extrusion detoxification technique on flaxseed by uniform design optimization. Technology. 61:51–59. DOI: 10.1016/j.seppur.2007.09.016

Zmijewski JW, Landar A, Watanabe N, Dickinson DA, Noguchi N & Darley-Usmar VM. 2005. Cellsignaling by oxidized lipids and the role of reactive oxygen species in the endothelium. Biochemical Society Transactions, 33: 1385-1389. DOI: 10.1042/BST20051385.