Effects of Dietary Supplementation of Barley Malt Extract and Malt Vinegar on Growth Performance, Jejunal Morphology and Meat Quality of Broiler Chickens

Document Type: Original Paper


1 Department of Animal Science, College of Agriculture, Isfahan University of Technology, Isfahan, Iran

2 Department of Animal Biosciences, University of Guelph, Guelph, ON, Canada


The objective of this study was to evaluate the effects of dietary supplementation of barley malt extract and barley malt vinegar on growth performance, jejunal morphology and meat quality of broilers. A total of 600 day-old chicks were allocated to four dietary treatments to evaluate the effect of dietary inclusion of 0.2% barley malt extract alone or along with 0.4 or 0.8% of malt vinegar as well as the control group. The results of the experiment have shown that dietary supplementation of 0.2% malt extract along with 0.4% malt vinegar resulted in a higher average daily gain (P = 0.007) and European production efficiency factor (P= 0.028) concomitantly lower feed conversion ratio (P = 0.047) compared to the birds were fed the control diet or supplemented by 0.2% malt extract along with 0.8% malt vinegar. Dietary supplementation of 0.2% malt extract increased the apparent absorption of surface area in the jejunum (P = 0.024). Moreover, dietary supplementation of malt extracts and malt vinegar alleviated malondialdehyde formation in the breast and thigh muscles (P< 0.05). Ceca microflora enumeration did not differ among the dietary treatments. In conclusion, dietary supplementation of 0.2% malt extract along with 0.4% malt vinegar may improve broiler growth performance and alleviate lipid oxidation.


AOAC (Association of Official Analytical Chemists). 2000. Official methods of analysis. Association of Official Analytical Chemists, Arlington, VA.
Akbari Moghaddam Kakhki R, Bakhshalinejad R, Hassanabadi A& Ferket PR. 2017. Effects of dietary organic zinc and α-tocopheryl acetate supplements on growth performance, meat quality, tissues minerals, and α-tocopherol deposition in broiler chickens. PoultryScience,96: 1257-1267.DOI: 10.3382/ps/pew386
Biagi G, Fusaro I, Pezzi P & Formigoni A. 2007. Effect of dietarysupplementation with malt extracts on milk production. Italian Journal of Animal Science, 6:260-262. DOI: 10.4081/ijas.2007.1s.260
Carías D& Millán N. 1996. Brewery waste as a substitute for soy protein in soy-brewer's yeast mixtures to feed broiler chickens. Archivos Latinoamericanos De Nutricion,46: 67-70.
De Verdal H, Mignon-Grasteau S, Jeulin C, Le Bihan-Duval E, Leconte M, Mallet S, Martin C & Narcy A. 2010. Digestive tract measurements and histological adaptation in broiler lines divergently selected for digestive efficiency. Poultry Science, 89: 1955-1961. DOI: 10.3382/ps.2010-813
Dibner, J., Buttin, P., 2002. Use of organic acids as a model to study the impact of gut microflora on nutrition and metabolism. The Journal of Applied Poultry Research, 11: 453-463. DOI:10.1093/japr/11.4.453
Ekinci R& Kadakal C. 2005. Determination of seven water-soluble vitamins in tarhana, a traditional Turkish cereal food, by high-performance liquid chromatography. Acta Chromatographica,15: 289-297
Gálvez MC, Barroso CG& Pérez-Bustamante JA. 1994. Analysis of polyphenolic compounds of different vinegar samples. Zeitschrift für Lebensmittel-Untersuchung und Forschung, 199:29-31. DOI:10.1007/BF01192948
Hernández F, GarcíaV, Madrid J, Orengo J, Catalá P& Megias M. 2006. Effect of formic acid on performance, digestibility, intestinal histomorphology and plasma metabolite levels of broiler chickens. British Poultry Science,47: 50-56. DOI:10.1080/00071660500475574
Hosseini SJ, Nassiri Moghaddam H& Kermanshahi H. 2010. The influence of different levels of brewers spent grain and enzyme on performance and digesta viscosity of broiler chicks. Journal of Animal and Veterinary Advances, 9: 2608-2612.DOI: 10.3923/javaa.2010.2608.2612
Jones D& Greenshields R. 1969. Volatile constituents of vinegar. I. A survey of some commercially available malt vinegars. Journal of the Institute of Brewing,75: 457-463. DOI:10.1002/j.2050-0416.1969.tb06382.x
Leeson S, Namkung H, Antongiovanni M& Lee E. 2005. Effect of butyric acid on the performance and carcass yield of broiler chickens. Poultry Science, 84: 1418-1422. DOI:10.1093/ps/84.9.1418
Maillard MN, Soum MH, Boivin P& Berset C. 1996. Antioxidant activity of barley and malt: relationship with phenolic content. LWT-Food Scienceand Technology, 29: 238-244. DOI:10.1006/fstl.1996.0035
Maneewan B& Yamauchi K. 2004. Intestinal villus recovery in chickens refed semi-purified protein, fat, or fibre-free pellet diets. British Poultry Science,45: 163-170.DOI: 10.1080/00071660410001715759
Medina E, Romero C, Brenes M, de Castro A. 2007. Antimicrobial activity of olive oil, vinegar, and various beverages against foodborne pathogens. Journal of Food Protection, 70:1194-1199. DOI: 10.4315/0362-028X-70.5.1194
Menconi A, Kuttappan V, Hernandez-Velasco X, Urbano T, Matté F, Layton S, Kallapura G, Latorre J, Morales B& Prado O. 2014. Evaluation of a commercially available organic acid product on body weight loss, carcass yield, and meat quality during preslaughter feed withdrawal in broiler chickens: A poultry welfare and economic perspective. PoultryScience,93: 448-455.DOI: 10.3382/ps.2013-03444
Myers RL. 2007. The 100 most important chemical compounds: a reference guide. Journal of Chemical Education, 86:1182.DOI: 10.1021/ed086p1182
Qingming Y, Xianhui P, Weibao K, Hong Y, Yidan S, Li Z, Yanan Z, Yuling Y, Lan D& Guoan L. 2010. Antioxidant activities of malt extract from barley (Hordeum vulgare L.) toward various oxidative stress in vitro and in vivo. Food Chemistry, 118: 84-89.DOI:10.1016/j.foodchem.2009.04.094
Ricke S. 2003. Perspectives on the use of organic acids and short chain fatty acids as antimicrobials. PoultryScience,82: 632-639.DOI: 10.1093/ps/82.4.632
Samanya M& Yamauchi Ke. 2001. Morphological Changes of the Intestinal Villi in Chickens Fed the Dietary Charcoal Powder Including Wood Vinegar Compounds. The Journal of Poultry Science, 38: 289-301. DOI: 10.2141/jpsa.38.289
Smulikowska S, CzerwiƄski J, Mieczkowska A. 2010. Effect of an organic acid blend and phytase added to a rapeseed cake containing diet on performance, intestinal morphology, caecal microflora activity and thyroid status of broiler chickens. Journal of AnimalPhysiology and Animal Nutrition,94: 15-23.DOI: 10.1111/j.1439-0396.2008.00876.x
Yoshimura H& Hayakawa T. 1993. Promoting effect of wood vinegar compounds on the mycelial growth of two basidiomycete. Mycoscience, 34: 141-151. DOI:10.1007/BF02268554
Zhang L, Cao G, Zeng X, Zhou L, Ferket PR, Xiao Y, Chen A& Yang C. 2014. Effects of Clostridium butyricum on growth performance, immune function, and cecal microflora in broiler chickens challenged with Escherichia coli K88. PoultryScience, 93: 46-53.DOI: 10.3382/ps.2013-03412
Zhao X, He X, Yang X& Zhong X. 2013. Effect of Portulaca oleracea extracts on growth performance and microbial populations in ceca of broilers. Poultry Science,92: 1343-1347.DOI: 10.3382/ps.2012-02434
Zheng W& Wang SY. 2001. Antioxidant activity and phenolic compounds in selected herbs.Journal of Agricultural and Food Chemistry,49: 5165-5170.DOI: 10.1021/jf010697n