Allelic Polymorphism of GH, GHR and IGF-1 Genes and Their Association with Growth and Carcass Traits in Mazandaran Native Fowl

Document Type: Original Paper

Authors

1 Department of Animal Science, College of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran

2 1Department of Animal Science, College of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran

3 Department of Genetic and Animal Breeding, Faculty of Animal Science, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran

4 Golestan Agricultural and Natural Resources Research and Education Center, Gorgan, Iran

Abstract

This investigation aimed to study polymorphism of growth hormone (GH), growth hormone receptor (GHR), and insulin like growth factor 1 (IGF-1) genes and their associations with growth and carcass traits in Mazandaran native chicken. 200 male chicks were reared and slaughtered at 12 weeks of age. Traits including live weight at 4, 8 and 12 weeks of age, body weight and weights of heart, liver, gizzard, spleen, and abdominal fat, pH of meat, water holding capacity of the meat and intramuscular fat were recorded. Before dissecting birds, blood samples were collected and DNA was extracted. The frequency of alleles (+ and -) were determined to be 0.627 and 0.373 for GH locus, 0.715 and 0.285 for GHR locus, and 0.407 and 0.593 for IGF-1 gene locus, respectively. Hardy-Weinberg equilibrium study using Chi-square test showed that the studied population was not in Hardy-Weinberg equilibrium. Analysis of phenotypic and genotypic data showed a significant association between genotypes of GH gene and live weights at 8 and 12 weeks of age and carcass weight. Significant associations were found between the GHR gene with live weight at 12 weeks of age and carcass weight and also between IGF-1 gene with abdominal fat and intramuscular fat (P < 0.05). Generally, it can be concluded that GH, GHR and IGF-1 genes can be used as candidate genes for improving growth and carcass traits in Mazandaran native fowls.

Keywords


Asghari Ghelghachi A, Seyedabadi HR & Lak A. 2013. Association of growth hormone gene polymorphism with growth and fatness traits in Arian broilers. International Journal of Biosciences, 12: 216-220.

Bai JY, Zhang Q & Jia XP. 2006. Comparison of different foreground and background selection methods in marker-assisted introgression. Acta Genetica Sinica, 33: 1073-1080. DOI: 10.1016/S0379-4172(06)60144-3

Dehghanzadeh H, Mirhosseini SZ & Shadparvar A. 2004. Study on DNA polymorphism of Iranian native chickens population using RAPD markers. Journal of Pajouhesh-Va-Sazandegi, 17: 2-9. [In Persian with English abstract]

Emara MG & Kim H. 2003. Genetic markers and their application in poultry breeding. Poultry Science, 82: 952-957. DOI:  10.1093/ps/82.6.952

Enayati B & Rahimi Mianji G. 2011. Effect of polymorphism of some candidate genes from growth hormone axis on egg production traits in Mazandaran native fowls. Iranian Journal of Animal Science Research, 3: 304-310. [In Persian with English abstract]

Garrett AJ, Rincon G, Medrano JF, Elzo MA, Silver GA & Thomas MG. 2008. Promoter region of the bovine growth hormone receptor gene: Single nucleotide polymorphism discovery in cattle and association with performance in Brangus bulls. Journal of Animal Science, 86: 3315-3323. DOI: 10.2527/jas.2008-0990

Ghazikhani Shad A, Nejati Javaremi A & Mehrabani Yeganeh H. 2007. Animal model estimation of genetic parameters for most important economic traits in Iranian native fowls. Journal of Biological Sciences, 10: 2787-2789.

Havenstein GB, Ferket PR & Qureshi MA. 2003. Growth, livability, and feed conversion of 1957 versus 2001 broilers when fed representative 1957 and 2001 broiler diets. Poultry Science, 82: 1500-1508. DOI: 10.1093/ps/82.10.1500

Hoffmann I, Siewerdt F & Manzella D. 2004. Research and investment-challenges and options for sustainable use of poultry genetic resources. 22th World Poultry Congress. CD Proceedings. Pages, 8-13.

Kadlec J, Hosnedlova B, Rehout V, Citek J & Hanusova L. 2011. Insulin-like growth factor-I gene polymorphism and its association with growth and slaughter characteristics in broiler chickens. Journal of Agrobiology, 28: 157-163.

Lei MM, Nie QH, Peng X, Zhang DX & Zhang XQ. 2005. Single nucleotide polymorphisms of the chicken insulin-like factor binding protein 2 gene associated with chicken growth and carcass traits. Poultry Science, 84: 1191-1198. DOI: 10.1093/ps/84.8.1191

Naghavi MR, Ghariazi B & Hosseini Salekdeh G. 2013. Molecular Markers. 4nd Ed. Tehran University Press. Tehran. 340 Pages. [In Persian]

Nie Q, Sun B, Zhang D, Luo C, Ishag NA, Lei M, Yang G & Zhang X. 2005. High diversity of chicken growth hormone gene and effects on growth and carcass traits. Journal of Heredity, 96: 698-703. DOI: 10.1093/jhered/esi114

Ouyang JH, Xie L, Nie Q, Luo C, Liang Y, Zeng H & Zhang X. 2008. Single nucleotide polymorphism (SNP) at the GHR gene and its associations with chicken growth and fat deposition traits. British Poultry Science, 49: 87-95. DOI: 10.1080/00071660801938817

Piryonesi A, Mardani K, Khakpour K, Ghaderzadeh M & Modaresi R. 2012. Study on the polymorphism of insulin-like growth factor 1 gene (IGF1) in West Azerbaijan native chickens. Journal of Novin Genetic, 7: 417-419. [In Persian]

Wang Q. 2010. Study on relationships between GH, GHR gene polymorphism and performance in quail. Master's thesis. Northeast Agricultural University, China.

Zhou H, Mitchell AD, McMurtry JP, Ashwell CM & Lamont SJ. 2005. Insulin-like growth factor-I gene polymorphism associations with growth, body composition, skeleton integrity, and metabolic traits in chickens. Poultry Science, 84: 212–219. DOI: 10.1093/ps/84.2.212