Department of Animal Science, Faculty of Agriculture, University of Zanjan, Zanjan, Iran.
This experiment was conducted to investigate the effect of phytase superdoses alone or in combination with citric acid (CA) in canola meal-based diets severely limited in available phosphorus (Pa) on growth performance, plasma phosphorus (P), and tibia ash (TA) in broilers from 22 to 42 d of age. Two hundreds and eighty 21-d-old male broilers were used in 28 pens of 10 birds per each. The experimental diets consisted of a positive control (PC) diet and six negative control (NC) diets which consisted of two levels of CA (0 and 20 g/Kg) and three levels of phytase (0, 1000 and 4000 U/Kg) in a 2 × 3 factorial arrangement. The PC diet contained 4.3 g/Kg Pa, but all NC diets contained 1.5 g/Kg Pa. Results indicated that the birds fed the PC diet had a significantly higher average daily gain (ADG), plasma P and TA, but a lower feed conversion ratio (FCR) than those fed the NC diet. The ADG, FCR and plasma P values in birds fed NC diets supplemented with 4000 U/Kg phytase enzyme (with or without CA) significantly reached those of birds fed the PC diet. But, addition of phytase enzyme at 1000 U/Kg only plus CA to the NC diet could significantly improve FCR and plasma P. A significant interaction was observed between phytase and CA for FCR and plasma P. Although TA values in NC + 1000 U/Kg phytase treatments (with or without CA) were similar to the PC treatment, TA values of NC + 4000 U/Kg phytase treatments (with or without CA) was greater than that of the PC treatment. Results of this study showed that, in severely limited Pa corn-canola meal-based diets, supplementing 4000 U/Kg phytase or also 1000 U/Kg phytase plus CA will be sufficient to obtain the comparable feed efficiency in broilers to those fed the adequate Pa diet.
Aviagen. 2009. Ross 308 Broiler Nutrition Specifications. http://en.aviagen.com/ross-308/.
Chung TK, Rutherfurd SM, Thomas DV & Moughan PJ. 2013. Effect of two microbial phytases on mineral availability and retention and bone mineral density in low-phosphorus diets for broilers. British Poultry Science, 54: 362-373.
Cowieson AJ, Wilcock P & Bedford MR. 2011. Super-dosing effects of phytase in poultry and other monogastrics. World's Poultry Science Journal, 67: 225-235.
Dilger RN, Onyango EM, Sands JS & Adeola O. 2004. Evaluation of microbial phytase in broiler diets. Poultry Science, 83: 962-970.
Gehring CK, Bedford MR & Dozier WA. 2013. Extra-phosphoric effects of phytase with and without xylanase in corn-soybean meal-based diets fed to broilers. Poultry Science, 92: 979-991.
Han JC, Yang XD, Qu HX, Xu M, Zhang T, Li WL, Yao JH, Liu YR, Shi BJ, Zhou ZF & Feng XY. 2009. Evaluation of equivalency values of microbial phytase to inorganic phosphorus in 22- to 42-day-old broilers. Journal of Applied Poultry Research, 18: 707-715.
Jendza JA, Dilger RN, Sands JS & Adeola O. 2006. Efficacy and equivalency of an Escherichia coli-derived phytase for replacing inorganic phosphorus in the diets of broiler chickens and young pigs. Journal of Animal Science, 84: 3364-3374.
Leske KL & Coon CN. 1999. A bioassay to determine the effect of phytase on phytate phosphorus hydrolysis and total phosphorus retention of feed ingredients as determined with broilers and laying hens. Poultry Science, 78: 1151–1157.
Liu N, Ru YJ, Cowieson AJ, Li FD & Cheng XCH. 2008. Effects of phytate and phytase on the performance and immune function of broilers fed nutritionally marginal diets. Poultry Science, 87: 1105-1111.
Liu N, Ru Y, Wang J & Xu T. 2010. Effect of dietary sodium phytate and microbial phytase on the lipase activity and lipid metabolism of broiler chickens. British Journal of Nutrition, 103: 862-868.
Maenz DD, Engele-Schaan CM, Newkirk RW & Classen HL. 1999. The effect of minerals and mineral chelators on the formation of phytase-resistant and phytase-susceptible forms of phytic acid in solution and in a slurry of canola meal. Animal Feed Science and Technology, 81: 177-192.
NRC (National Research Council). 1994. Nutrient Requirements of Poultry. 9th Rev. Ed. National Academy Press. Washington, DC. 176 Pages.
Olukosi OA, Cowieson AJ & Adeola O. 2007. Age-related influence of a cocktail of xylanase, amylase, and protease or phytase individually or in combination in broilers. Poultry Science, 86: 77-86.
Parmer TG, Carew LB, Alster FA & Scanes CG. 1987. Thyroid function, growth hormone, and organ growth in broilers deficient in phosphorus. Poultry Science, 66: 1995-2004.
Pirgozliev V, Acamovic T & Bedford MR. 2009. Previous exposure to dietary phytase reduces the endogenous energy losses from precision-fed chickens. British Poultry Science, 50: 598-605.
Pirgozliev V & Bedford MR. 2013. Energy utilisation and growth performance of chicken fed diets containing graded levels of supplementary bacterial phytase. British Journal of Nutrition, 109: 248-253.
Pirgozliev V, Karadas F, Pappas A, Acamovic T & Bedford MR. 2010. The effect on performance, energy metabolism and hepatic carotenoid content when phytase supplemented diets were fed to broiler chickens. Research in Veterinary Science, 89: 203-205.
Pirgozliev V, Oduguwa O, Acamovic T & Bedford MR. 2007. Diets containing Escherichia coli-derived phytase on young chickens and turkeys: Effects on performance, metabolizable energy, endogenous secretions, and intestinal morphology. Poultry Science, 86: 705-713.
Pirgozliev V, Oduguwa O, Acamovic T & Bedford MR. 2008. Effects of dietary phytase on performance and nutrient metabolism in chickens. British Poultry Science, 49: 144-154.
Radcliffe JS, Zhang Z & Kornegay ET. 1998. The effects of microbial phytase, citric acid, and their interaction in a corn-soybean meal-based diet for weanling pigs. Journal of Animal Science, 76: 1880-1886.
Rutherfurd SM, Chung TK, Thomas DV, Zou ML & Moughan PJ. 2012. Effect of a novel phytase on growth performance, apparent metabolizable energy, and the availability of minerals and amino acids in a low-phosphorus corn-soybean meal diet for broilers. Poultry Science, 91: 1118-1127.
SAS (Statistical Analysis System). 2003. SAS/STAT® 9.1. User’s Guide. SAS Institute Inc. Cary, North Carolina.
Selle PH & Ravindran V. 2007. Microbial phytase in poultry nutrition. Animal Feed Science and Technology, 35: 1-41.
Shaw AL, Hess JB, Blake JP & Ward NE. 2011. Assessment of an experimental phytase enzyme product on live performance, bone mineralization, and phosphorus excretion in broiler chickens. Journal of Applied Poultry Research, 20: 561-566.
Shirley RB & Edwards HM. 2003. Graded levels of phytase past industry standards improves broiler performance. Poultry Science, 82: 671-680.
Simon O & Igbasan F. 2002. In vitro properties of phytases from various microbial origins. International Journal of Food Science and Technology, 37: 813-822.
Snow JL, Baker DH & Parsons CM. 2004. Phytase, citric acid, and 1α-hydroxycholecalciferol improve phytate phosphorus utilization in chicks fed a corn-soybean meal diet. Poultry Science, 83: 1187-1192.
Taheri HR, Heidari A & Shahir MH. 2015. Effect of high-dose phytase supplementation in broilers from 22 to 42 days post-hatch given diets severely limited in available phosphorus. British Poultry Science, 56: 330-336.
Walk CL, Bedford MR & McElroy AP. 2012. Influence of limestone and phytase on broiler performance, gastrointestinal pH, and apparent ileal nutrient digestibility. Poultry Science, 91: 1371-1378.
Walk CL, Bedford MR, Santos TS, Paiva D, Bradley JR, Wladecki H, Honaker C & McElroy AP. 2013. Extra-phosphoric effects of superdoses of a novel microbial phytase. Poultry Science, 92: 719-725.
Walk CL, Santos TT & Bedford MR. 2014. Influence of superdoses of a novel microbial phytase on growth performance, tibia ash, and gizzard phytate and inositol in young broilers. Poultry Science, 93: 1172-1177.
Woyengo TA, Guenter W, Sands JS, Nyachoti CM & Mirza MA. 2008. Nutrient utilisation and performance responses of broilers fed a wheat-based diet supplemented with phytase and xylanase alone or in combination. Animal Feed Science and Technology, 146: 113-123.
Woyengo TA, Slominski BA & Jones RO. 2010. Growth performance and nutrient utilization of broiler chickens fed diets supplemented with phytase alone or in combination with citric acid and multicarbohydrase. Poultry Science, 89: 2221-2229.
Zhang ZB, Kornegay ET, Radcliffe JS, Denbow DM, Veit HP & Larsen CT. 2000. Comparison of genetically engineered microbial and plant phytase for young broilers. Poultry Science, 79: 709-717.
Please cite this article as: Taheri HR & Taherkhani S. 2015. Effect of phytase superdoses and citric acid on growth performance, plasma phosphorus and tibia ash in broilers fed canola meal-based diets severely limited in available phosphorus. Poult. Sci. J. 3 (1): 27-36.