Effects of Drinking Thyme Essence (Thymus vulgaris L.) on Growth Performance, Immune Response and Intestinal Selected Bacterial Population in Broiler Chickens

Document Type: Original Paper

Authors

Department of Animal Science, Bu-Ali Sina University, Hamedan, Iran.

Abstract

This study evaluated the effects of Drinking Thyme Essence (DTE) (Zero, 0.10, 0.15 and 0.20 mL/L) on growth performance, immune response and changing of intestinal bacterial population in broiler chickens. A total number of 500 day old male broiler chicks (Ross 308), were randomly assigned to 4 treatments with 5 replicates and 25 chickens per each, based on a completely randomized design (CRD). Growth performances were assessed during the range of 8-21, 22-42 and 8-42 d. At 21 and 42 d blood serum titers including: Newcastle Disease (ND), Avian Influenza (AI), Infectious Bronchitis Virus (IBV) and Infectious Bursal Disease (IBD) were sampled. Bacterial populations in intestinal digesta were determined at the age of 21 and 42 d. DTE levels significantly (P) improved total weight gain and total feed conversion ratio  as compared with the control group during 1-42 d of age. The titer of serum antibodies did not show significant differences between different treatments at the 21 or 42 d. Total count, E. coli, and Gram negative bacteria (GNB) at the age of 21 and 42 days showed a significantly (P) lower number compared with the control group. There was a significantly (P) higher number of Lactic Acid Bacteria (LAB) in DTE groups compared with control group at both ages of 21 and 42 d. In conclusion, different levels of DTE (especially at level of 0.20 mL/L) could improve the growth performance, immune response and intestinal lactic acid bacteria as a health index during different growth periods.

Keywords


Alcicek A, Bozkurt M & Cabuk M. 2003. The effect of an essential oil combination derived from selected herbs growing wild in Turkey on broiler performance. South African Journal Animal  Science, 33: 89-94.

Al-Jaff FK. 2011. Effect of coriander seeds as diet ingredient on blood parameters of broiler chicks raised under high ambient temperature. International Journal of Poultry Science, 10: 82-86.

Black JG. 1996. Microbiology: Principles and Applications. 3rd Ed. Prentice Hall. New Jersey. 790 Pages.

Bolukbasi SC & Erhan MK. 2007. Effect of dietary thyme (Thymus Vulgaris) on laying hens performance and Escherichia coli (E.coli) concentration in feces. International Journal of Natural and Engineering Sciences, 1 : 55-58.

Chevallier A. 1996. The Encyclopedia of Medicinal Plants. DK Publishing. Michigan. 336 Pages.

Czifra G, Nilsson M, Alexander DJ, Manvell R, Kechskemeti S & Engstrom BE. 1996. Detection of PMV-1 specific antibodies with a monoclonal antibody blocking enzyme-linked immunosorbent assay. Avian Pathology, 25: 691–703.

Cook NC & Samman S. 1996. Flavonoids chemistry, metabolism, cardio protective effects and dietary sources. Journal of Nutrition and Biotecnology, 7: 66-76.

Cross DE, Hillman K, Fenlon D, Deans SG, McDevitt RM & Acamovic T. 2004. Antibacterial properties of phytochemicals in aromatic plants in poultry diets. In: Acamovic T, Stewart C & Pennycott TW. (Eds). Poisonous Plants and Related Toxins. CAB International. Wallingford. Oxon. Pages, 175-180.

Cross DE, McDevitt RM, Hilman K & Acamovic T. 2007. The effect of herbs and their associated essential oils on performance, dietary digestibility and gut microflora in young chicks from 7 to 28 days of age. British Poultry Science, 48: 496-504.

Dorman HJD & Deans SG. 2000. Antimicrobial agents from plants: Antibacterial activity of plant volatile oils. Journal of Application Microscopy, 88: 308-316.

Griggs JP & Jacob JP. 2005. Alternatives to antibiotics for organic poultry production. Journal of Applied Poultry Research, 14: 750-756.

Ghazalah AA & Ali AM. 2008. Rosemary leaves as a dietary supplement for growth in broiler chickens. International Journal of Poultry Science, 7: 234-239.

Gulfraz M, Mehmood S, Minhas N, Jabeen N, Kausar R, Jabeen K & Arshad G. 2008. Composition and antimicrobial properties of essential oil of Foeniculum Vulgare. African  Journal of  Biology, 7 : 4364-4368.

Grashorn MA. 2010. Use of phytobiotics in broiler nutrition – an alternative to infeed antibiotics. Jounal of Animal and  Feed Science, 19: 338-347.

Hertrampf JW. 2001. Alternative antibacterial performance promoters. Poultry International, 40: 50-52.

Hoffman PD & Wu C. 2010. The effect of thymol and thyme oil feed supplementation on growth performance, serum antioxidant levels and cecal  Salmonella population in broilers. Journal of Applied Poultry Research, 19: 432-443.

Hashemi SR & Davoodi H. 2010. Phytogenic as new class of feed additive in poultry industry. Journal of Animal and Veterinary Advances, 9: 2295-2304.

Herawati T. 2010. The effect feeding red ginger as phytogenic on body weight gain, feed conversion and internal organs condition of broiler. International Journal of Poultry Science, 9: 963-967.

Jamroz D, Orda J, Kamel C, Wiliczkiewicz A, Wertelecki T & Skorupinska J. 2003. The influence of phytogenic extracts on performance, nutrient digestibility, carcass characteristics, and maicrobial status in broiler chickens. Journal of Animal and Feed  Sciences, 12: 583-596.

Jamroz D, Williczkiewicz A, Werteleck T, Orda J & Skorupinska J. 2005. Use of active substances of plant origin in chicken diet based on maiz and locally grown cereals. British Poultry Science, 46: 458-493.

Langhout P. 2000. New additives for broiler chickens. World Poultry, 16: 22-27.

Manach F. Regerat F & Texier O. 1996. Bioavailability, metabolism and physiological impact of 4-oxo-flavonoids. Nutrition Resserch, 16: 517-544.

Mitsch P, Kohler B, Gabler C, Losa R & Zitterl-Eglseer K. 2002. CRINA Poultry reduces the colonisation and proliferation of Clostridium perfrigens in the intestine and feces of broiler chickens. Proceedings of the 11th European Poultry Conference. Bremen. Page, 113

Najafi P & Torki M. 2010. Performance, blood metabolites and immunocompetaence of broiler chicks fed diets included essential oils of medicinal herbs. Journal of Animal and Veterinary Advances, 9: 1164-1168.

Rehman J, Dillow JM, Carter SM, Chou J, Le B & Maisel AS. 1999. Increased production of antigren-specific immunoglobulins G and M following in vivo treatment with medicinal plants Echinacea angustifolia and Hydratis canadensis. Immunity Letter, 68: 391-395.

Safa SEG & AL-Beitawi NA. 2009. The effect of feeding of crushed thyme (Thymus valgaris) on growth, blood constituents, gastrointestinal tract and carcass characteristics of broiler chickens. Journal of  Poultry Science, 4: 100-104.

SAS (Statistical Analysis System). 2004. SAS/STAT® 9.2. User's Guide. SAS Institute Inc. Cary, North Carolina.

Savage TF, Cotter PF & Zakrzewska EI. 1996. The effect of feeding mannan oligosaccharide on immunoglobulins, plasma IgG and bile IgA of Wrolstad MW male turkeys. Poultry Science, 75: 143-149.

Toghyani M, Tohidi M, Gheisari AB & Tabeidian SA. 2010. Performance, immunity, serum biochemical and hematological parameters in broiler chicks fed dietary thyme as alternative for an antibiotic growth promoter. African Journal of Biology, 9: 6819-6825.

Tortora GJ, Funke BR & Case CL. 1995. Microbiology. An Introduction. 5th Ed. Benjamin-Cummings Publishing Company. Redwood. 801 Pages.

Williams P & Losa R. 2001. The use of essential oils and their compounds in poultry nutrition. World Poultry, 17: 14-15.

Please cite this article as: Saki AA, Kalantar M & Khoramabadi V. 2014. Effects of drinking Thyme essence (Thymus vulgaris L.) on growth performance, immune response and intestinal selected bacterial population in broiler chickens. Poult. Sci. J. 2 (2): 113-123.