Humoral Immune Response Induced by PLGA Micro Particle Coupled Newcastle Disease Virus Vaccine in Chickens

Document Type: Original Paper

Authors

1 Institute of Animal Health and Veterinary Biologicals, Hebbal, Bangalore. Karnataka State. India.

2 Veterinary Dispensary, Dabbegatta Kaimara, Madhugiri Tq, Tumkur Dist. Karnataka. India.

3 Translational Research Platform for Veterinary Biologicals, TANUVAS, Chennai. Tamilnadu, India.

Abstract

This experiment was conducted for evaluating the humoral immune responses induced by Poly Lactide-co-Glycolide Acid (PLGA) microspheres coupled inactivated Newcastle Disease Virus (NDV) vaccine in comparison to an ‘in-house’ prepared inactivated and a live commercial vaccine. PLG microparticles containing inactivated NDV were prepared by a double emulsion technique based on solvent evaporation method. The size of the NDV coupled PLG microparticles was determined by Electron Microscopy. NDV coupled PLG microparticles were spherical having smooth surface, hollow core inside with no pores on the surface. The experiment was conducted in four groups of chickens (n=15). The encapsulation efficiency of NDV coupled PLG microparticles was determined by protein estimation and HA activity in elute. The mean (± SE) size of PLG microspheres was found to be 2.409 ± 0.65 µm. The mean percent of encapsulation efficiency of PLG microspheres coupled to NDV was assessed based on the total protein content and HA activity in elute was found to be 8.03 ± 0.50 and 12.5 ± 0.00, respectively. In conclusion, the results of the experiment showed that PLGA coupled NDV vaccine elicited stronger and prolonged humoral immune response in chickens, in comparison to the other tested vaccines, as assessed by haemagglutination inhibition and enzyme linked immuno sorbent asaay titers.

Keywords


Allan WH, Lancaster JE, & Toth B. 1978. Newcastle disease vaccine – Food and Agricultural Organization of the United Nations, Rome.

Coombes AGA, Lavellea EC & Davis SS. 1999. Biodegradable lamellar particles of poly (lactide) induce sustained immune responses to a single dose of adsorbed protein. Vaccine, 17: 2410-2422.

Dandapat S, Banerjee DR, Dash BB, Kataria JM & Yadav MP. 2003a. Immunogenicity of single intranasal dose of IBD vaccine based on poly-lactide-co-glycolide (PLG) microparticles in broiler chickens. Indian Journal of Comparative Microbiology Immunology and Infectious Diseases, 24: 173-179.

Dandapat S, Banerjee DR, Kataria JM, Ram GC & Yadav MP. 2003b. Induction of mucosal immunity in poultry after oral immunization with single dose of microencapsulated EDS-76 Virus.  Indian Journal of Comparative Microbiology Immunology and Infectious Diseases, 24: 181-185.

Delgado A, Lavelle EC, Hartshorne M & Davis SS. 1999. PLG microparticles stabilized using enteric coating polymers as oral vaccine delivery systems. Vaccine, 17: 2927-2938.

Eldridge JH, Staas JK, Meulbroek JA, Tice TR & Gilly RM. 1991.  Biodegradable and biocompatible poly (D,L-lactic-co-glycolic acid) micro spheres ad an adjuvant for Staphylococcal Enterotoxin B toxoid which enhances the level of toxin neutralizing antibodies.  Infection and Immunity, 59: 2978-2986.

Evans JT. Ward JR. Kern J & Johnson ME. 2004. A single vaccination with protein-microspheres elicits a strong CD8 T-cell-mediated immune response against Mycobacterium tuberculosis antigen Mtb8.4. Vaccine, 22: 1964–1972.

Feng L, Rong X, Zhou XJ, Maitani Y, Wang SC, Jiang Y & Nagai T. 2006. Pharmaceutical and immunological evaluation of a single-dose hepatitis B vaccine using PLGA micro spheres. Journal of Controlled Release, 112: 35–42.

Greenway TE, Eldridge JH, Ludwig G, Stass JE, Smith JF, Gilly RM & Michalek SM. 1998. Induction of protective immune responses against Venezuelan equine encephalitis (VEE) virus aerosol challenge with microencapsulated VEE virus vaccine. Vaccine, 15: 1314-1323.

Hans ML & Lowman AM. 2002. Biodegradable nanoparticles for drug delivery and targeting. Current Opinion in Solid State and Materials Science, 6: 319–327.

He Q, Mitchell AR, Johnson SL, Wagner BC, Morcol T & Bell SJD. 2000. Calcium phosphate nano particle adjuvant. Clinical Diagnostic Laboratory Immunology, 7: 899–903.

He Q, Mitchell AR, Johnson SL, Wagner BC, Morcol T & Bell SJD. 2002. Calcium phosphate nano particles induce mucosal immunity and protection against Herpes Simplex Virus type 2. Clinical Diagnostic Laboratory Immunology,
5: 1021-1024.

Hilbert AK, Fritzsche U & Kissel T. 1999. Biodegradable microsphere containing influenza A vaccine: immune response in mice. Vaccine, 17: 1065-1073.

Jayawardane GWL & Spradbrow PB. 1995. Mucosal immunity in chickens vaccinated with the V4 strain of Newcastle disease virus. Veterinary Microbiology, 46: 69 –76.

Kato Y, Onishi H & Machida Y. 2003. Application of chitin and chitosan derivatives in the pharmaceutical field. Current Pharmaceutical Biotechnology, 4: 303-309.

Kirubaharan JJ, Priyadarshini MLM, Suresh Babu S, Singh RK & Palaniswami KS. 2008. Single serum dilution ELISA for quantification of antibodies in chickens exposed to Newcastle disease virus. Indian Veterinary Journal, 85: 1143–1146.

Leong KW, Mao HQ, Truong-Le VL, Roy K, Walsh SM & August JT. 1998. DNA-polycation nano spheres as non-viral gene delivery vehicles. Journal of Controlled Release, 53: 183–193.

Nixon DF, Hioe C, Chen PD, Bian Z, Kuebler P & Li ML. 1996. Synthetic peptides entrapped in micro particles can elicit cytotoxic T cell activity. Vaccine,
14: 1523–1530.

OIE (Office International des Epizooties). 2004. Newcastle disease. Manual of standards for diagnostic test and vaccines. 4th Ed. Paris. OIE Chapter 2.1.15 

Praveen E, Diwan M, Cao M & Samuel J. 2004. Characterization of poly (D,L-lactic-co-glycolic acid) based nanoparticulate system for enhanced delivery of antigen to dendritic cells. Vaccine, 22: 2406-2412.

Reed LJ, & Muench H, 1938. A simple method for estimating 50 percent end points. American Journal of Hygiene, 27: 493-497.

Santander-Ortega MJ, Csaba N, Alonso MJ, Ortega-Vinuesa JL & Bastos-Gonzalez D. 2007. Stability and physiochemical characteristics of PLGA: poloxamine blend nano particles a comparative study. Colloids and surfaces A, 296: 132-140.

SPSS (Statistical Packages for the Social Sciences). 1996. SPSS for Windows Release 10.01. SPSS Inc. Chicago.

Please cite this article as: Sanganagouda K, Naveen AN, Shivaraj M & Dhinakarraj G . 2014. Humoral immune response induced by PLGA micro particle coupled Newcastle disease virus vaccine in chickens. Poult. Sci. J. 2 (1): 15-24.