Teratogenic effect of Disodium 5’ Ribonucleotide (E635) on 15th day chick embryo Gallus gallus (Research note)

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Document Type : Research Note

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Department of Zoology, Shivaji University, Kolhapur-416004, Maharashtra, India

Abstract

Disodium 5' ribonucleotide (E635), commonly used as a food additive, may pose teratogenic risks. The study aimed to evaluate the teratogenicity of disodium 5’ ribonucleotide using chick embryos as an in vivo model. Fertilized chicken eggs were inoculated with LD50 dose of 0.05 µg/egg of disodium 5’ ribonucleotide on the 0th, 5th, and 10th days of embryonic development. The control group received 0.05 mL of distilled water on the 0th, 5th, and 10th days. After 15 days of incubation, embryos were examined for morphological abnormalities. The disodium 5’ ribonucleotide inoculated embryos exhibited significant developmental anomalies, including acrania, monophthalmia, bent toes, curved beak, scissor beak, omphalocele, and hematoma, compared to the control group. These abnormalities were categorized and analyzed through morphometric assessments to evaluate their severity. Data were subjected to statistical analysis using t-test to find out significance of developmental anomalies in dose induced group and control group animals. Anatomical changes indicated that disodium 5’ ribonucleotide exposure led to severe teratogenic effects in chick embryos, which has highlighted potential risks associated with its use as a food additive.

Keywords

Poultry Science Journal

Articles in Press, Corrected Proof
Available Online from 01 June 2025

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