In ovo Inoculation of cLF36 on Post-hatch Performance, Intestinal Histo-morphometry and Microflora of Broiler Chickens Challenged with Clostridium perfringens

Document Type : Original Paper

Authors

Department of Animal Science, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran

Abstract

In ovo injection of camel lactoferrin (cLF36) as an antimicrobial peptide was applied in Ross 308 fertile eggs and tested in 320 post-hatched chickens challenged with Clostridium perfringens (Cp). In 8 treatments and five replicates of 8 birds each, performance, jejunum morphometry and ileal microbial counts of chickens were assayed. Feed intake and feed conversion ratio of the chickens affected by treatments. Together with the positive control group under the Cp (10 8 cfu/g) challenge and the negative control group under the antibiotic (AB) challenge, the highest villi length was observed. The highest crypt depth was related to the treatment with the Cp challenge and the lowest value was related to the in ovo injection of cLF36 group and combined Cp and AB challenges. The number of Clostridium spp. in the ileal contents increased in the chickens challenged with Cp (P < 0.05). The greatest change was observed in the treatment with injection of cLF36 during the embryonic period and challenge with Cp and the lowest value was related to negative control treatment. In addition, the difference between treatments with cLF36 in ovo injection during the embryonic period and challenge with or without Cp challenge was significantly increased. In the groups under the Cp challenge, the population of E. coli was numerically increased. Based on the obtained results, cLF36, derived from camel milk, could change some of the indices in performance. It caused morphological changes in the villi of ilium and caused a decrease the microbial counts of Clostridium spp., similar to the AB group in the chickens challenged with Cp. Our research attempts to create a new window for in ovo administration of cLF36, according to its beneficial effects in the present study, can be introduced as a candidate for growth-promoting antibiotics.

Keywords

References

Apajalahti J, Kettunen A & Graham H. 2004 Characteristics of the gastrointestinal microbial communities, with special reference to the chicken. World’s Poultry Science, 60: 223–232. DOI: 10.1079/WPS200415
Bao H, She R, Liu T, Zhang Y, Peng KS, Luo D, Yue Z, Ding Y, Hu Y, Liu W& Zhai L. 2009. Effects of pig antibacterial peptides on growth performance and intestine mucosal immune of broiler chickens. Poultry Science, 88: 291–297. DOI:10.3382/ps.2008-00330
Caly DL, D'Inca Romain, Auclair E & Drider D. 2015. Alternatives to antibiotics to prevent necrotic enteritis in broiler chickens: a microbiologist’s perspective. Frontiers in Microbiology, 6: 1-12. DOI: 10.3389/fmicb.2015.01336
Castanys-Muñoz E, Martin MJ & Vazquez E. 2016. Building a beneficial microbiome from birth. Advances in Nutrition, 7: 323–30.  DOI:10.3945/an.115.010694
 Caspary WF. 1992. Physiology and pathophysiology of intestinal absorption. American Journal of Clinical Nutrition, 55: 299–308.  DOI:10.1093/ajcn/55.1.299s
Choi SC, Ingale SL, Kim JS, Park YK, Kwon IK & Chae BJ. 2013. Effects of dietary supplementation with an antimicrobial peptide-P5 on growth performance, nutrient retention, excreta and intestinal microflora and intestinal morphology of broilers. Animal Feed Science and Technology, 185(1-2): 78–84. DOI: 10.1016/j.anifeedsci.2013.07.005
Daneshmand A, Kermanshahi H, Sekhavati MH, Javadmanesh A & Ahmadian M. 2019. Antimicrobial peptide, cLF36, affects performance and intestinal morphology, microflora, junctional proteins, and immune cells in broilers challenged with E. coli. Scientific Reports, 9 (1) DOI: 10.1038/s41598-019-50511-7
Daneshmand A, Kermanshahi H, Sekhavati MH, Javadmanesh A, Ahmadian M, Alizadeh M & Aldavoodi A. 2020. Effects of cLFchimera, a recombinant antimicrobial peptide, on intestinal morphology, microbiota, and gene expression of immune cells and tight junctions in broiler chickens challenged with C. perfringens. Scientific Reports, 10: 17704. DOI: 10.1038/s41598-020-74754-x
Hu F, Gao X, She R, Chen J, Mao J, Xiao P & Shi R. 2017. Effects of antimicrobial peptides on growth performance and small intestinal function in broilers under chronic heat stress. Poultry Science, 96: 798–806. DOI: 10.3382/ps/pew379
Javadmanesh A,  Mohammadi E,  Mousavi Z, Azghandi M & Tanhaeian A .2021. Antibacterial effects assessment on some livestock pathogens, thermal stability and proposing a probable reason for different levels of activity of thanatin. Scientific Reports, 11(1): 10890. DOI: 10.1038/s41598-021-90313-4.
Kermanshahi H, Heravi RM, Attar A, Abbasi Pour AR, Bayat E, Hossein Zadeh M, Daneshmand A & Ibrahim SA. 2017. Effects of acidified yeast and whey powder on performance, organ weights, intestinal microflora, and gut morphology of male broilers. Brazilian Journal of Poultry Science, 19: 309 –316. DOI: 10.1590/1806-9061-2016-0351
Khodambashi Emami N, Daneshmand A, Naeini SZ, Graystone EN & Broom LJ. 2017. Effects of commercial organic acid blends on male broilers challenged with E. coli K88: performance, microbiology, intestinal morphology, and immune response. Poultry Science, 96, 3254–3263.  DOI: 10.3382/ps/pex106
Kiela. Powel R and Ghishan. Fayez K.2016. Physiology of intestinal absorption and secretion. Best practice & research Clinical gastroenterology, 30(2): 145–159. DOI:10.1016/j.bpg.2016.02.007
Lai Y & Gallo LR. 2009. AMPed up immunity: How antimicrobial peptides have multiple roles in immune defense. Trends in Immunology, 30(3): 131–141.  DOI:10.1016/j.it.2008.12.003
Liu T, She R, Wang K, Bao H, Zhang Y, Luo D, Hu Y, Ding Y, Wang D & Peng K. 2008. Effects of rabbit Sacculus rotundus antimicrobial peptides on the intestinal mucosal immunity in chickens. Poultry Science, 87: 250–254. DOI:  10.3382/ps.2007-00353
Matsuda K, Chaudhari A & Lee HJ. 2010. Avian colibacillosis caused by an intestinal pathogenic Escherichia coli isolate from calf diarrhea. Research in Veterinary Science, 89: 150–152. DOI:  10.1016/j.rvsc.2010.02.008
Ocana VS, De Ruiz Holgado AAP & Nader-Macias ME. 1999. Growth inhibition of Staphylococcus aureus by H2O2-producing Lactobacillus paracasei subsp. paracasei isolated from the human vagina. FEMS Immunology and Medical Microbiology, 23: 87–92. DOI: 10.1007/pl00006802
Ohh SH, Shinde PL, Choi JY, Jin  Z , Hahn TW , Lim HT , Kim GY, Park YK , Hahm KS  & Chae BJ. 2010. Effects of potato (Solanum tuberosum L. cv. golden valley) protein on performance, nutrient metabolizability, and cecal microflora in broilers. European Poultry Science (EPS), 74: 30–35. DOI:10.3382/ps.2008-00491
Olkowski AA, Wojnarowicz C, Chirino-Trejo M & Drew MD. 2005. Responses of broiler chickens orally challenged with Clostridium perfringens isolated from field cases of necrotic enteritis. Research in Veterinary Science, 81: 99–108. DOI: 10.1016/j.rvsc.2005.10.006
Popoff MR. 2013. Clostridium, in Sécurité Sanitaire des Aliments: Epidémiologie et lutte Contre les Contaminants Zoonotiques, eds D. Drider and G. Salvat (Paris: Economica), 165–189.
Proctor A & Phillips GJ. 2019. Differential effects of bacitracin methylene disalicylate (BMD) on the distal colon and cecal microbiota of young broiler chickens. Frontiers in Veterinary Science, 6: 114. DOI: 10.3389/fvets.2019.00114.
Reicher N, Melkman-Zehavi T, Dayan JA. Wong E & Uni Z. 2022. Nutritional Stimulation by In-ovo Feeding Modulates Cellular Proliferation and Differentiation in the Small Intestinal Epithelium of Chicks. Animal Nutrition, 8: 91-101.  DOI: 10.1016/j.aninu.2021.06.010
Songer JG. 1996. Clostridial enteric diseases of domestic animals. Clinical Microbiology Reviews, 9: 216–234. DOI: 10.1128/cmr.9.2.216
Tahmoorespur M, Azghandi M, Javadmanesh A, Meshkat Z & Sekhavati MH. 2019. A Novel chimeric anti‑HCV peptide derived from camel lactoferrin and molecular level insight on its interaction with E2. International Journal of Peptide Research and Therapeutics, 3: 1593-1605. DOI: 10.1007/s10989-019-09972-7
Tang J, Ghazali FM, AbdulAziz S, Nishibuchi M & Radu S. 2009. Comparison of thermophilic Campylobacter spp. occurrence in two types of retail chicken samples. International Food Research Journal, 16: 277–288.
Tanhaeian A, Shahriari Ahmadi F, Sekhavati MH & Mamarabadi M. 2018a. Expression and purification of the main component contained in camel milk and its antimicrobial activities against bacterial plant pathogens. Probiotics and Antimicrobial Proteins, 10(4): 787-793. DOI: 10.1007/s12602-018-9416-9
Tanhaeian A, Azghandi M, Razmyar J, Mohammadi E & Sekhavati MH. 2018b. Recombinant production of a chimeric antimicrobial peptide in E. coli and assessment of its activity against some avian clinically isolated pathogens. Microbial Pathogenesis, 122: 73–78. DOI:10.1016/j.micpath.2018.06.012
Tanhaeian A, Jaafari MR, Shahriari Ahmadi F, Vakili‐Ghartavol R & Sekhavati MH. 2018c. Secretory expression of a chimeric peptide in Lactococcus lactis: assessment of its cytotoxic activity and a deep view on its interaction with cell-surface glycosaminoglycans by molecular modeling. Probiotics and Antimicrobial Proteins. 11(3): 1034-1041. DOI: 10.1007/s12602-018-9496-6
Timbermont L, Haesebrouck F, Ducatelle R & Van Immerseel F. 2011. Necrotic enteritis in broilers: an updated review on the pathogenesis. Avian Pathology. 40: 341–347. DOI: 10.1080/03079457.2011.590967
Van Immerseel F, De Buck J, Pasmans F, Huyghebaert G, Haesebrouck F & Ducatelle R. 2004. Clostridium perfringens in poultry: an emerging threat for animal and public health. Avian Pathology, 33: 537–549. DOI: 10.1080/03079450400013162
 Wang S, Xiangfang Z, Qing Y & Shiyan Q. 2016. Antimicrobial peptides as potential alternatives to antibiotics in food animal industry. International Journal of Molecular Sciences, 17: 603. DOI: 10.3390/ijms17050603
Wu Y, Shao Y, Song B, Zhen W, Wang Z, Guo Y, Shahid MS & Nie W. 2018. Effects of Bacillus coagulans supplementation on the growth performance and gut health of broiler chickens with Clostridium perfringens induced necrotic enteritis. Poultry Science, 97: 2654–2666. DOI: 10.3382/ps/pey119
Yoon JH, Ingale SL , Kim JS , Kim KH, Lohakare J, Kyung Park Y, Cheol Park J, Kwon IK & Chae BJ. 2013. Effects of dietary supplementation with antimicrobial peptide-P5 on growth performance, apparent total tract digestibility, faecal and intestinal microflora and intestinal morphology of weanling pigs. Journal of the Science of Food Agriculture, 93: 587–592. DOI: 10.1002/jsfa.5840
Poultry Science Journal
Volume 12, Issue 2
September 2024
Pages 237-245

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