Flock Uniformity, Blood Indices, and Nutrient Retention of Broiler Chickens Fed Low Energy and Protein Diets Supplemented with Multi-Enzyme

Document Type : Original Paper


Department of Animal Science and Technology, Federal University of Technology, Owerri – PMB 1526. Owerri, Imo State, Nigeria


A feeding trial was conducted using two-hundred-day-old male Ross-308 broiler chickens to evaluate the effect of multi-enzyme (Natuzyme®) on flock uniformity, some haematological and serum biochemical indices, and nutrient retention in broiler chickens fed low energy and protein (LEP) diets. The birds were randomly assigned to four experimental groups of 50 birds each in a completely randomized design. There were five replicates for each treatment group, with 10 chicks per replicate. The first group (positive control/PC) received a standard diet without multi-enzyme supplementation; whereas the LEP0, LEP0.25 and LEP0.50 groups received low-energy-protein diet (LEP) supplemented with multi-enzyme at 0 (negative control), 0.25, and 0.50 g/kg feed, respectively. The PC group received a standard diet having energy and protein of (3000 kcal/kg and 23%) and  (3200 kcal/kg and 20%) at starter and finisher phases respectively. Other groups received LEP diets having energy and protein of the standard diet decreased by 100 kcal/kg and 0.60% both at starter and finisher phases. The feeding trial lasted for 42 days. On days 21 and 42 of the experiment, birds under the LEP0  and LEP0.25 groups had lower (P < 0.05) flock uniformity compared to those of PC and LEP0.50 groups. There was no significant effect of the dietary treatments on haematological and serum biochemical indices of broiler chickens. Birds fed LEP0 and LEP0.25 diets had significantly (P < 0.05) low metabolizable energy (ME), crude fiber (CF) and crude protein (CP) retention compared with the PC group. On the other hand, Broiler chickens offered the LEP0.50 diet had improved (P < 0.05) apparent ME, CF and CP retention.  Results of the study suggest that multi-enzyme supplementation at 0.50 g/kg to low energy and protein broiler chicken diet improved flock uniformity as well as metabolizable energy, CF and protein retention without adverse effects on haematological indices and serum, metabolites.


Abdel-Daim ASA, Tawfeek SS, El-Nahass ES, Hassan AHA & Youssef IMI. 2020. Effect of Feeding Potato Peels and Sugar Beet Pulp With or Without Enzyme on Nutrient Digestibility, Intestinal Morphology, and Meat Quality of Broiler Chickens. Poultry Science Journal. 8: 189-199. DOI: 10.22069/psj.2020.17876.1560
Abudabos AM.  2012. Effect of enzyme supplementation to normal and low-density broiler diets based on corn-soybean meal. Asian Journal of Animal Veterinary Advances, 7: 139-148. DOI: 10.3923/ajava.2012.139.148
Ac-Chukwuocha NB, Ngah S & Chukwuocha A. 2017. Vulnerability studies of sensitive watershed areas of Owerri South East Nigeria using digital elevation models. Journal of Geoscience and Environment Protection, 5: 1-10. DOI: 10.4236/gep.2017.510001
Ahiwe EU, Omede AA, Abdullah ME & IJI PA. 2018. Managing dietary energy intake by broiler chickens to reduce production cost and improve product quality. Animal Husbandry and Nutrition. Banu Yiicel and Turgay Taskin. Intech Open. DOI: 10.5772/Intechopen.76972
Ahiwe EU, Chang’a EP, Abdallh M, Al-Qahtani ME, Kheraviia SK, Wua S, Grahamc H & Iji PA. 2019. Dietary hydrolysed yeast cell wall extract is comparable to antibiotics in the control of subclinical necrotic enteritis in broiler chickens. British Poultry Science, 60: 757-765. DOI: 10.1080/00071668.2019.1664727
Alabi OO, Shoyombo AJ, Akpor OB, Olumba OM & Adeyonu AG. 2019. Exogenous enzymes and the digestibility of nutrients by broilers: A mini review. International Journal of Poultry Science, 18: 404-409. DOI: 10.3923/ijps.2019.404.409
Anjum MS & Chaudhry AS.  2010. Using enzymes and organic acids in broiler diets, The Journal of Poultry Science, 47: 97-105. DOI: 10.2141/jpsa.009082
Ao Z & Choct M. 2013. Oligosaccharides affect performance and gut development of broiler chickens. Asian-Australasian Journal of Animal Science, 26: 116-121. DOI: 105713/ajas.2012.12414
AOAC (Association of Official Analytical Chemists). 2007. Official methods of analysis (18th ed.). Washington, DC, USA.
Aviagen. 2018. Ross 308 Management Handbook. https://en.aviagen.com/assets/Tech_Center/Ross Broiler/Ross-BroilerHandbook2018-EN.pdf.
Aviagen. 2019. Ross 308 Nutrient specifications. 0429-AVNR-035. https://en.aviagen.com/assets/Tech_Center/Ross_Broiler/RossBroilerNutritionSpecs2019-EN.pdf.
Bounous D & Stedman N. 2000. Normal avian hematology: chicken and turkey. In: Feldman BF, Zinkl JG, Jain NC, editors. Schalm's veterinary hematology. New York: Wiley; 2000. Pages, 1147-1154. DOI: 10.1590/1519/6984.03414
Cowieson AJ & Kluenter AM. 2019. Contribution of exogenous enzymes to potentiate the removal of antibiotic growth promoters in poultry production. Animal Feed Science and Technology, 250: 81-92. DOI: 10.1016/j.anifeedsci.2018.04.026
Dosković V, Bogosavljević-bosković S, Pavlovski Z, Milosević B, Skrbić Z, Rakonjac S & Petricević V. 2013. Enzymes in broiler diets with special reference to protease. World’s Poultry Science Journal, 69: 343-360. DOI: 10.1017/S0043933913000342
Esonu BO. 2015. Animal Nutrition and Feeding- A Functional Approach, Publishers R & R Associates, Owerri. 3rd Edition.
Iwuji TC, Nwapi DO, Ogbuewu IP, Kadurumba EO, Egenuka FC & Okere PC, 2017. Management, blood and reproductive parameters of rabbits reared in Imo State. Nigerian  Journal of  Animal Production, 44: 230 – 247. DOI:10.51791/njap.v44i2.1017
Iyayi EA & Davies BI. 2005.  Effect of Enzyme Supplementation of Palm Kernel Meal and Brewer’s Dried Grain on the Performance of Broilers. International Journal of Poultry Science, 4: 76-80. DOI: 10.3923/ijps.2005.76.80
Jackson CJ, Gillam EM, Ollis DL. 2010. Evolution of enzyme. In: Comprehensive natural products II, edited by Hung-Wen (Ben) Liu, Lew Mander, Elsevier Ltd. DOI: 10.1016/B978-008045382-8.00675-4
Jackson ME, Geronian K, Knox A, McNab J & McCartney E. 2004. A dose-response study with the feed enzyme beta-mannanase in broilers provided with corn-soybean meal based diets in the absence of antibiotic growth promoters. Poultry Science, 83: 1992-1996. DOI: 10.1093/ps/83.12.1992
Low AG. 1990. Protein evaluation in pigs and poultry: In:  feed evaluation. Wiseman, J and Cole, D.J.A. (Editors), Bullerworths, London. Pages, 91-114.
Meluzzi A, Primiceri G, Giordani R & Fabris G. 1992. Determination of blood constituents reference values in broilers. Poultry Science, 71: 337-345. DOI: 10.3382/ps.0710337
Minitab 17 Statistical Software. 2013. [Computer Software] State College, PA, USA: Minitab, Inc.
Moftakharzadeh SA, Janmohammadi H, Taghizadeh A, Kianfar R & Olyayee ME. 2019. Effect of enzyme addition on energy utilization and performance of broiler chickens fed wheat-based diet with different metabolizable energy levels, Acta Scientiarum. Animal Sciences, 41: e44585. DOI: 10.4025/actascianimsci.v41i1.44585
Mohammadigheisar M & Kim IH. 2018. Addition of a protease to low crude protein density diets of broiler chickens. Journal of Applied Animal Research, 46: 1377-1381. DOI: 10.1080/09712119.2018.1512862
NRC. 1994. Nutrient requirements of poultry9ed. Washington: National Academy of Sciences. 155p.
Ochei J & Kolhatkar A. 2007. Medical Laboratory Science, Theory and Practice, Tata McGraw-Hill Publishing Company Limited, New Delhi, India. Pages, 90-200.
Palic D, Okanovic D, Psodorov D, Dzinic N, Slobodan lilac S, Zekic V & Milic D. 2012. Prediction of metabolizable energy of poultry feeds by estimating in vitro organic matter digestibility. African Journal of Biotechnology, 11: 7313-7317.
Ravindran V.  2013. Feed enzymes: The science, practice, and metabolic realities. Journal of Applied Poultry Research, 22:628-636. DOI: 10.3382/japr.2013-00739
Salami RI & Odunsi AA. 2019. Performance of broiler chickens fed 8% crude fiber diets at three energy levels with or without enzyme during the starter and finisher phases. International Journal of Poultry Science, 18: 423-430. DOI: 10.3923/ijps.2019.423.430
Sarica S, Yurtseven S & Pola I. 2020. Supplementing diets for broilers that are low in crude protein and amino acids with protease. South African Journal of Animal Science, 50: 521-528.
Schader C, Muller A, Scialabba NE, Hecht J, Isensee A, Erb K, Smith P, Makkar HPS, Klocke P, Leiber F, Schwegler P, Stolze M & Niggli U. 2015. Impacts of feeding less food-competing feedstuffs to livestock on global food system sustainability. Journal of the Royal Society Interface, 12: 1-12. DOI: 10.1098/rsif.2015.0891
Souza KMR, Araujo RB, Sakamoto MI, Santos TT, Kikuchi CG, Nakashima DT & Caelano VC. 2014. Performance and morphometry of the intestinal mucosa of laying hens fed diets containing xylanase. Brazilian Journal of Poultry Science, 16: 241-248. DOI: 10.1590.1516-635x1603241-248
Thanapal P, Hong IK & Kim H. 2021. Influence of low and high-density diets with yeast supplements on feed intake, nutrient digestibility, egg production and eff quality in Hy-line brown laying hens. Brazilian Journal of Poultry Science, 23:1-8. DOI: 10.1590/1806-9061-2020-1370
Thirumalaisamy G, Muralidharan J, Senthilkumar S, Sayee RH & Priyadharsini M. 2019. Cost-effective feeding of poultry. International Journal of Environmental Science and Technology, 5: 3997-4005.
Vasdal G, Dranquist EG, Skjerve E, deJomg IC, Berg C, Michel V & Moe RO. 2019. Associations between carcass weight uniformity and production measures on farm and at slaughter in commercial broiler flocks. Poultry Science, 98: 4261-4268. DOI: 10: 3382/ps/pez252
Zhao Y, Tian G, Chen D, Zheng P, Yu J, He J, Mao X, Huang Z, Luo Y, Luo J & Yu B. 2019. Effect of different dietary protein levels and amino acids supplementation patterns on growth performance, carcass characteristics and nitrogen excretion in growing-finishing pigs. Journal of Animal Science and Biotechnology, 10:75-81. DOI: 10.1186/s40104-019-0381-2