Comparison of the Effects of Dietary Probiotic and Prebiotic with Sesame Meal Processed by Bio-fermentation Technique on Production Performance, Serum Biochemical Metabolites, and Gut Microbial Population of Laying Hens

Document Type : Original Paper


Department of Defense Science and Technology, Institute of Defense Technology and Passive Defense, University and Higher Institute for National Defense and Strategic Research, Tehran, Iran


This experiment was conducted to investigate and compare the effects of dietary probiotic and prebiotic with sesame meal processed by bio-fermentation technique (SMP) on production performance, blood metabolites, and gut microbiota population in laying hens. A total of 200 Hy-Line W-36 laying hens, 43-wk-old, were randomly allocated to four experimental treatments with five replicates and ten hens per replicate. Experimental treatments were as follows: 1) corn-soybean meal diet (as control diet; [CON]), 2) control diet + 0.1% probiotic (PRO), 3) control diet + 0.1% prebiotic (PRE), and 4) a diet without additives, in which SMP replaced part of the soybean meal in the control diet (SMP). The bio-fermentation process decreased the pH, phytic acid, and crude fiber and increased the crude protein content and the number of lactic acid bacteria (LAB) in sesame meal (P < 0.05). The feeding trial results showed that hens fed diets containing SMP and PRO had better egg production, egg mass, and feed conversion ratio compared to other treatments (P < 0.05). Lactic acid bacteria population and pH in the crop of birds that received PRO and SMP were higher and lower than in other birds, respectively (P < 0.05). Hens fed diets containing SMP, PRO, and PRE had greater LAB populations and lower coliform count in the ileum and cecal than those fed the CON diet (P < 0.05). Feeding diets containing tested feed additives and SMP decreased serum concentrations of cholesterol and triglycerides (P < 0.05). In summary, the results indicated that using SMP in laying hens' diet improved laying production, gut microbiota balance, and serum lipid profile. Therefore, it can be concluded that bio-fermentation, in addition to providing the possibility of replacing dietary sesame meal for soybean meal in laying hen diets, can be applied as a strategic tool for promoting gastrointestinal hygiene and health in birds.


AOAC. 2007. Official Methods of Analysis of AOAC International. 18th ed. Association of Official Analytical Chemists, Washington, DC.
Barbarestani SY, Jazi V, Mohebodini H, Ashayerizadeh A, Shabani A & Toghyani M. 2020. Effects of dietary lavender essential oil on growth performance, intestinal function, and antioxidant status of broiler chickens. Livestock Science, 233: 103958. DOI: 10.1016/j.livsci.2020.103958
Canibe N & Jensen BB. 2012. Fermented liquid feed—Microbial and nutritional aspects and impact on enteric diseases in pigs. Animal Feed Science and Technology, 173(1-2): 17-40. DOI: 10.1016/j.anifeedsci.2011.12.021
Chiang G, Lu WQ, Piao XS, Hu JK, Gong LM & Thacker PA. 2009. Effects of feeding solid-state fermented rapeseed meal on performance, nutrient digestibility, intestinal ecology and intestinal morphology of broiler chickens. Asian-Australasian Journal of Animal Sciences, 23(2): 263-271. DOI: 10.5713/ajas.2010.90145
Chen W, Zhu XZ, Wang JP, Wang ZX, & Huang YQ. 2013. Effects of Bacillus subtilis var. natto and Saccharomyces cerevisiae fermented liquid feed on growth performance, relative organ weight, intestinal microflora, and organ antioxidant status in Landes geese. Journal of Animal Science, 91(2): 978-985. DOI: 10.2527/jas.2012-5148
Ding XM, Li DD, Bai SP, Wang JP, Zeng QF, Su ZW & Zhang KY. 2018. Effect of dietary xylooligosaccharides on intestinal characteristics, gut microbiota, cecal short-chain fatty acids, and plasma immune parameters of laying hens. Poultry Science, 97(3): 874-881. DOI: 10.3382/ps/pex372
Engberg RM, Hammersh M, Johansen NF, Abousekken MS, Steenfeldt S & Jensen BB. 2009. Fermented feed for laying hens: effects on egg production, egg quality, plumage condition and composition and activity of the intestinal microflora. British Poultry Science, 50(2), 228-239. DOI: 10.1080/00071660902736722
El Jeni R, Dittoe DK, Olson EG, Lourenco J, Corcionivoschi N, Ricke SC & Callaway TR. 2021. Probiotics and potential applications for alternative poultry production systems. Poultry Science, 100(7): 101156. DOI: 10.1016/j.psj.2021.101156
Forte C, Acuti G, Manuali E, Proietti PC, Pavone S, Trabalza-Marinucci M & Franciosini MP. 2016. Effects of two different probiotics on microflora, morphology, and morphometry of gut in organic laying hens. Poultry Science, 95(11): 2528-2535. DOI: 10.3382/ps/pew164
Hassan MS, Soltan MA & Abdel-Moez AM. 2015. Nutritive value of soybean meal after solid state fermentation with Saccharomyces cerevisiae for Nile tilapia, Oreochromis niloticus. Animal Feed Science and Technology, 201: 89-98. DOI: 10.1016/j.anifeedsci.2015.01.007
Heres L, Engel B, Van Knapen F, De Jong MC, Wagenaar JA & Urlings HA. 2003. Fermented liquid feed reduces susceptibility of broilers for Salmonella enteritidis. Poultry Science, 82(4): 603-611. DOI: 10.1093/ps/82.4.603
Jahanian R & Ashnagar M. 2015. Effect of dietary supplementation of mannan-oligosaccharides on performance, blood metabolites, ileal nutrient digestibility, and gut microflora in Escherichia coli-challenged laying hens. Poultry Science, 94(9): 2165-2172. DOI: 10.3382/ps/pev180
Jazi V, Foroozandeh AD, Toghyani M, Dastar B & Koochaksaraie RR. 2018. Effects of Pediococcus acidilactici, mannan-oligosaccharide, butyric acid and their combination on growth performance and intestinal health in young broiler chickens challenged with Salmonella Typhimurium. Poultry Science, 97(6): 2034-2043. DOI: 10.3382/ps/pey035
Jazi V, Farahi M, Khajali F, Abousaad S, Ferket P & Assadi Soumeh E. 2020. Effect of dietary supplementation of whey powder and Bacillus subtilis on growth performance, gut and hepatic function, and muscle antioxidant capacity of Japanese quail. Journal of Animal Physiology and Animal Nutrition, 104(3): 886-897. DOI: 10.1111/jpn.13323
Jha R, Das R, Oak S & Mishra P. 2020. Probiotics (direct-fed microbials) in poultry nutrition and their effects on nutrient utilization, growth and laying performance, and gut health: a systematic review. Animals, 10(10): 1863. DOI: 10.3390/ani10101863
Missotten JA, Michiels J, Dierick N, Ovyn A, Akbarian A & De Smet S. 2013. Effect of fermented moist feed on performance, gut bacteria and gut histo-morphology in broilers. British Poultry Science, 54(5): 627-634. DOI: 10.1080/00071668.2013.811718
Missotten JA, Michiels J, Degroote J & De Smet S. 2015. Fermented liquid feed for pigs: an ancient technique for the future. Journal of Animal Science and Biotechnology, 6(1): 1-9. DOI: 10.1186/2049-1891-6-4
Mohebodini H, Jazi V, Ashayerizadeh A, Toghyani M & Tellez-Isaias G. 2021. Productive parameters, cecal microflora, nutrient digestibility, antioxidant status, and thigh muscle fatty acid profile in broiler chickens fed with Eucalyptus globulus essential oil. Poultry Science, 100 (3): 100922. DOI: 10.1016/j.psj.2020.12.020
Mukhopadhyay NA. 1999. Effect of fermentation on the nutritive value of sesame seed meal in the diets for rohu, Labeo rohita (Hamilton), fingerlings. Aquaculture Nutrition. 5: 229-236.
Niba AT, Beal JD, Kudi AC & Brooks PH. 2009. Potential of bacterial fermentation as a biosafe method of improving feeds for pigs and poultry. African Journal of Biotechnology, 8(9).
Olude O, George F & Alegbeleye W. 2016. Utilization of autoclaved and fermented sesame (Sesamum indicum L.) seed meal in diets for Til-aqua natural male tilapia. Animal Nutrition, 2(4): 339-344. DOI: 10.1016/j.aninu.2016.09.001
Ranjitkar S, Karlsson AH, Petersen MA, Bredie WLP, Petersen JS & Engberg RM. 2016. The influence of feeding crimped kernel maize silage on broiler production, nutrient digestibility and meat quality. British Poultry Science, 57(1): 93-104. DOI: 10.1080/00071668.2015.1115468
Shabani A, Boldaji F, Dastar B, Ghoorchi T, Zerehdaran S & Ashayerizadeh A. 2021. Evaluation of increasing concentrations of fish waste silage in diets on growth performance, gastrointestinal microbial population, and intestinal morphology of broiler chickens. Animal Feed Science and Technology, 275: 114874. DOI: 10.1016/j.anifeedsci.2021.114874
Shirani V, Jazi V, Toghyani M, Ashayerizadeh A, Sharifi F & Barekatain R. 2019. Pulicaria gnaphalodes powder in broiler diets: consequences for performance, gut health, antioxidant enzyme activity, and fatty acid profile. Poultry Science, 98(6): 2577-2587. DOI: 10.3382/ps/pez010
Sjofjan O, Adli DN, Sholikin MM, Jayanegara A & Irawan A. 2021. The effects of probiotics on the performance, egg quality and blood parameters of laying hens: A meta-analysis. Journal of Animal and Feed Sciences. 30 (1): 11-18. DOI: 10.22358/jafs/133432/2021
Sun H, Tang JW, Yao XH, Wu YF, Wang X & Feng J. 2013. Effects of dietary inclusion of fermented cottonseed meal on growth, cecal microbial population, small intestinal morphology, and digestive enzyme activity of broilers. Tropical Animal Health and Production, 45(4): 987-993. DOI: 10.1007/s11250-012-0322-y
Sumarsih S., Yudiarti T, Utama CS, Rahayu ES & Harmayani E. 2010. The influence of using fish fermented by lactic acid bacteria as feed substitution on serum lipid profile of broilers. Journal of the Indonesian Tropical Animal Agriculture, 35(2): 124-128. DOI: 10.14710/jitaa.35.2.124-128
Tang SGH, Sieo CC, Ramasamy K, Saad WZ, Wong HK & Ho YW. 2017. Performance, biochemical and haematological responses, and relative organ weights of laying hens fed diets supplemented with prebiotic, probiotic and synbiotic. BMC Veterinary Research, 13(1): 1-12. DOI: 10.1186/s12917-017-1160-y
Wang Y, Deng Q, Song D, Wang W, Zhou H, Wang L & Li A. 2017. Effects of fermented cottonseed meal on growth performance, serum biochemical parameters, immune functions, antioxidative abilities, and cecal microflora in broilers. Food and Agricultural Immunology, 28(4): 725-738. DOI: 10.1080/09540105.2017.1311308
Zaghari M, Sarani P & Hajati H. 2020. Comparison of two probiotic preparations on growth performance, intestinal microbiota,    nutrient digestibility and cytokine gene expression in broiler chickens. Journal of Applied Animal Research, 48(1): 166-175. DOI: 10.1080/09712119.2020.1754218
Zhang G, Wang H, Zhang J, Tang X, Raheem A, Wang M & Qin T. 2021. Modulatory effects of Bacillus subtilis on the performance, morphology, cecal microbiota and gut barrier function of laying hens. Animals, 11(6): 1523. DOI: 10.3390/ani11061523