Effect of Scrophularia striata Extract on Performance, Intestinal Microbial and Histo-morphometry, and Blood Parameters in Broilers under Normal or Challenged Condition with E. Coli

Document Type : Original Paper

Authors

Department of Animal Science, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran

Abstract

The effect of different levels of Scrophularia striata extract in normal and challenged conditions with E. coli on performance, carcass characteristics, cellular immune response, blood antioxidant status, intestinal histo-morphometry, and microbial population (E.coli and Lactobacillus) of Cobb 500 broilers were evaluated. The experiment was performed in a complete block design with a 2 × 4 factorial arrangement with five replications of 10 birds each for 35 days in two separated halls [include challenge (C) and non-challenge (N)] with similar experimental conditions. Experimental treatments in two breeding halls were: 1) basal diet or control (CONT), 2) basal diet + 0.1 g/kg of herbal extract (EXTR 1), 3) basal diet +0.2 g/kg of herbal extract (EXTR 2), 4) Basal diet +0.1 g/kg an antibiotic (Oxytetracycline) (ANTB). On the 16th and 24th days of the experiment, one dose of 1×107 CFU K99 E. Coli was gavaged to chickens in a challenged conditioned hall. The results showed that average body weight and daily weight gain in the whole period (days 35 and 1-35) in EXTR 2 was better than the control treatment (P < 0.05). The percentage of breast weight in the ANTB was significantly different from the control treatment (P < 0.05). Glutathione peroxidase (GPX) in EXTR 2 was better than the control treatment (P < 0.05). Malondialdehyde (MDA) in EXTR 1 was significantly lower than the control group (P < 0.05). The values of E. coli in the treatment ANTB were less than control treatment (P < 0.05). Lactobacillus value in treatment EXTR 2 was higher than control treatment (P < 0.05). The value of total immunoglobulin in 28 days in ANTB, EXTR 1, and EXTR 2 were significantly higher than that of control treatment (P < 0.05). The length of villi was affected by treatments (P < 0.05). In conclusion, dietary inclusion of 0.2 g/kg of Scrophularia striata extract may improve the health status of the birds during E. coli challenge.

Keywords

References

  1. Al-Kassie GAM. 2010. The role of peppermint (Menthe piperita) on performance in broiler diets. Agriculture and Biology Journal of North America, 1: 1009-1013. DOI: 10.5251/abjna. 2010.1.5.1009. 1013

    Amirghofran Z, Azadbakht M & Karimi MH. 2000. Evaluation of the immunomodulatory effects of five herbal plants. Journal of Ethnopharmacology, 72: 167-172. DOI: 10.1016/s0378-8741(00)00234-8

    Asani S, Majidzadehheravi R, Kermanshahi H & Vakili A. 2015. The effect of two probiotic supplements on intestinal morphology and blood monocyte activity of broilers in challenge with E.coli k99. 1st International Congress on the Development of Agricultural Science and Natural Resources. Poland, 7 Des 2015.

    Azadegan Mehr M, Hassanabadi A, Nassiri Moghadam H & Kermanshahi H. 2014. Supplementation of clove essential oils and probiotic to the broiler’s diet on performance, carcass traits and blood components. Iranian Journal of Applied Animal Science, 1: 117-122.

    Babri S, Doosti MH, Fatehi L & Salari AA. 2012.  The effects of Scrophularia striata extract on anxiety and depression behaviors in adult male mice. Pharmaceutical Sciences, 18: 133-140.

    Bains BS. 1996. The role of vitamin C in stress management. Misset World Poultry, 12: 38-41.

    Barbour EK, Saade MF, Nour AMA, Kayali G, Kodess S, Ghannam R & Shaib H. 2011. Evaluation of essential oils in the treatment of broilers co-infected with multiple respiratory etiologic agents. The International Journal of Applied Research in Veterinary Medicine, 4: 317-333.

    Beier RC, Byrd II JA, Kubena LF, Hume ME, McReynolds JL, Anderson RC & Nisbet DJ. 2014. Evaluation of linalool, a natural antimicrobial and insecticidal essential oil from basil: Effects on poultry. Poultry Science, 93: 267–272. DOI: 10.3382/ps.2013-03254

    Brenes A & Roura E. 2010. Essential oils in poultry nutrition: Main effects and modes of action. Animal Feed Science and Technology, 158: 1-14. DOI: 10.1016/j.anifeedsci.2010.03.007

    Burkholder KM, Thompson KL, Einstein ME, Applegate TJ & Patterson JA. 2008. Influence of stressors on normal intestinal microbiota, intestinal morphology, and susceptibility to salmonella entritidis colonization in broilers. Poultry Science, 87: 1734-1741. DOI: 10.3382/ps.2008-00107

    Burt S. 2004. Essential oils: their antibacterial properties and potential applications in foods – a review. International Journal of Food Microbiology. 94: 223-253. DOI: 10.1016/j. ijfoodmicro.2004.03.022

    Cabuk M, Alcicek A, Bozkurt M & Imer N. 2003. Antimicrobial properties of the essential oils isolated from aromatic plants and using possibility as alternative feed additives. Proceedings of the 2nd National Animal Nutrition Congress; 2003 Sep 8-20; Konya. Turquia. p. 184-187.

    Calislar S, Gemci I & Kamalak A. 2009. The effect of feeding thyme sage and rosmary oil on laying hen performance, cholesterol and some proteins ratio of egg yolk and Escherichia coli count feces. Archiv Fur Geflugelkunde, 72: 231-237.

    Cheema MA, Qureshi MA & Havenstein GB. 2003. A comparison of the immune response of a 2001 commercial broiler with a 1957 randombred broiler strain when fed representative 1957 and 2001 broiler diets. Poultry Science, 82: 1519–1529. DOI: 10.1093/ps/82.10.1519

    Chorianopoulos N, Kalpoutzakis E, Aligiannis N, Mitaku S, Nychas GJ & Haroutounian SA. 2004. Essential oils of satureja, Origanum, and Thymus species: chemical composition and antibacterial activities against food borne pathogens. Journal of Agricultural and Food Chemistry, 52: 8261-8267. DOI: 10.1021/jf049113i

    Cross DE, Mcdevith RM, Hilman K & Acamovic T. 2007. The effect of herbs and their associated essential oils on performance, digesibilities and gut microflora in chickens 7 to 28 d of age. British Poultry Science, 48: 496-506. DOI: 10.1080/ 00071660701463221

    Debersac P, Vernevaut MF, Amito MJ, Suschetet M & Siess MH. 2001. Effects of a water-soluble extract of rosemary and its purified component rosmarinic acid on xenobiotic metabolizing enzymes in rat liver. Food and Chemical Toxicology, 29: 109-117. DOI: 10.1016/S0278-6915(00)00117-4

    Demir E, Kilinc K, Yildirim Y, Dincer F & Esecel H. 2008. Comparative effects of mint, sage, thyme and flavomycin in wheat-based broiler diets. Archivoc de Zootecnia, 11: 54-63.

    Erhan   MK,    Bolukbas    SC    &   Urusan    H.  2012.

          Biological activities of pennyroyal (Mentha pulegium L.) in broilers. Livestock Science, 146: 189-192. DOI: 10.1016/j.livsci.2012.01.014

    Faramarzi S, Bozorgmehrifard MH, Khaki A, Moomivand H, Ezati MS, Rasoulinezhad S, Bahnamiri AJ & Dizaji B. 2013. Study on the effect of Thymus vulgaris essential oil on humoral immunity and performance of broiler chickens after La Sota vaccination. Annals of Biological Research, 6: 290-294.

    Garcia V, Catala-Gregori P, Hernandez F, Megias MD & Madrid J. 2007. Effect of formic acid and plant extracts on growth, nutrient digestibility, intestine mucosa morphology, and meat yield of broilers. Journal of Applied Poultry Research, 16: 555-562. DOI: 10.3382/japr.2006-00116

    Ghazanfari S, Mohammadi Z & Adib moradi M. 2015. Effects of coriander essential oil on the performance, blood characteristics, intestinal microbiota and histological of broilers. Brazilian Journal of Poultry Science, 17: 419-426. DOI: 10.1590/1516-635X1704419-426

    Gopi M, Karthik K, Manjunathachar HV, Tamilmahan P, Kesavan M, Dashparakash M, Balaraju BL & Purushothaman MR. 2014. Essential oils as a feed additive in poultry nutrition. Advances in Animal and Veterinary Sciences, 1: 1-7.

    Gray HG, Paradis TJ & Chang PW. 1989. Research note: Physiological effects of adrenocorticotropic hormone and hydrocortisone in laying hens. Poultry Science, 68: 1710-1713. DOI: 10.3382/ ps.0681710

    Gross WB & Siegel HS. 1983. Evaluation of the heterophil/lymphocyte ratio as a measure of stress in chickens. Avian diseases, 27: 972-979. DOI: 10.2307/1590198

    Habibi R, Sadeghi G & Karimi A. 2014. Effect of different concentrations of ginger root powder and its essential oil on growth performance, serum metabolites and antioxidant status in broiler chicken under heat stress. British Poultry Science, 55: 228-237. DOI: 10.1080/00071668.2014.887830

    Helander IM, Alakomi HL, Latva-Kala K, Mattila – Sandholm T, Pol M, Smid EJ, Gorris LG & Von Wright A. 1998. Charactrrization of the action of selected essential oil components on gram negative bacteria. Journal Agricultural food chemistry, 46: 3590-3595.

    Hevener W, Routh PA & Almond GW. 1999. Effects of immune challenge on concentrations of serum insulin-like growth factor-1 and growth performance in pigs. Canadian Veterinary Journal, 40: 782-786.

    Jamroz D, Wertelecki T, Houszka M & Kamel C. 2006. Influence of diet type on the inclusion of plant origin active substances on morphological and histochemical characteristics of the stomach and jejunum walls in chicken. Journal of Animal Physiology and Animal Nutrition, 90: 255–268. DOI: 10.1111/j.1439-0396.2005.00603.x

    Jamroz D, Wiliczkiewicz A, Wertelecki T, Orad J & Scorupinska J. 2005. Use of active substances of plant origin in chicken diets based on maize and domestic grains. British Poultry Science 46: 485-493. DOI: 10.1080/00071660500191056

    Janz JAM, Morel PCH, Wilkinson BHP & Purchas RW. 2007. Preliminary investigation of the effects of low-level dietary inclusion of fragrant essential oils and oleoresins on pig performance and pork quality. Meat Science, 75: 350-355. DOI: 10.1016/j.meatsci.2006.06.027

    Kamel C. 2001. Tracing modes of action and the roles of Plant extracts in non-ruminants. PP. 135-150. In: P.C. Garnsworthy and J. Wiseman (Eds.), Recent Advances in Animal Nutrition, Nottingham University Press, Nottingham.

    Kermanshahi H, Majidzadeh-Heravi R, Attar, A, Abbasi Pour, AR, Bayat E, Hosseinzadeh M & Salam Ibrahim A. 2017. Effects of acidified yeast and whey powder on performance, organ weights, intestinal microflora, and gut morphology of male broilers. Brazilian Journal of Poultry Science, 19: 309-316. DOI: 10.1590/1806-9061-2016-0351

    Khamisabadi H & Pourhesabi Q. 2018. The effect of the extract of two medicinal plants, Peppermint and Thyme in combination or separately on the performance of carcass characteristics and intestinal microflora of broilers. Iranian Journal of Animal Sciences, 117: 55-66 (In Persian).

    Kirkpinar F, Unulu HB & Ozdemir G. 2011. Effects of oregano and garlic essential oils on performance, carcass, organ and blood characteristics and intestinal microflora of broilers. Livestock Science, 137: 219-225. DOI: 10.1016/j.livsci.2010.11.010

    Lambert RJW, Skandamis PN, Coote PJ & Nychas GJE. 2001. A study of the minimum inhibitory concentration and mode of action of oregano essential oil, thymol and carvacrol. Journal of Applied Microbiology, 91: 453-462. DOI: 10.1046/j.1365-2672.2001.01428.x

    Lee KW, Everst H & Beynen AC. 2004. Essential oils in broiler nutrition. International Journal of Poultry Science, 12: 738-752.

    Lee KW, Kappert HJ, Frehner M, Losa R & Beynen AC. 2003. Effects of dietary essential oil components on growth performance, digestive enzymes and lipid metabolism in female broiler chickens. British Poultry Science, 44: 450-457. DOI: 10.1080/0007166031000085508

    Lin H, Sui J, Jiao HC, Buyes J & Decuypere E. 2006. Impaired development of broiler chickens by stress mimicked by corticosterone exposure. Comparative Biochemistry and Physiology-Part A: Molecular and Integrative Physiology. 143: 400-405. DOI: 10.1016/j.cbpa.2005.12.030

    Marcinčák S, Cabadaj  R,  Popelka  P &  Soltysova  L.

    1. Antioxidative effect of oregano supplemented to broiler on oxidative stability of poultry meat. Slovenian Veterinary Research, 45: 61-66.

    Michalina AS & Danuta S. 2017. Use of essential oils in broiler chicken production. A Review; Annals of Animal Science, 17: 317-335. DOI: 10.1515/aoas-2016-0046

    Mozafarian VA. 1999. Khuzastan flora: Agriculture natural resources research. Publication Center of Khuzestan.

    Ocak N, Erener G, Ak FB, Sungu M, Altop A & Ozmen A. 2008. Performance of broilers fed diets supplemented with dry peppermint (Mentha piperita L.) or thyme (thymus vulgaris L.) leaves as growth promoter source. Czech Journal of Animal Science, 53: 169-175.

    Paglia DE & Valentine WN. 1967. Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. The Journal of Laboratory and Clinical Medicine, 70: 158-169.

    Papageorgiou G, Botsoglou N, Govaris A, Giannenas I, Iliadis S & Botsoglou E. 2003. Effect of dietary oregano oil and α-tocopheryl acetate supplementation on iron-induced lipid oxidation of turkey breast, thigh, liver and heart tissues. Journal of Animal Physiology and Animal Nutrition, 87: 324-335. DOI: 10.1046/j.1439-0396.2003.00441.x

    Penalver P, Huerta B, Borge C, Astorga R, Romero R & Perea R. 2005. Antimicrobial activity of five essential oil against animal origin strains of the Enterobacteriaceae family. Acta Pathologica Microbiologica et Immunologica Scandinavica, 113:1-6. DOI: 10.1111/j.1600-0463.2005.apm 1130101.x

    Platel K & Srinivasan K. 2000. Influence of dietary spices and their active principles on pancreatic digestive enzymes in albino rats. Nahrung, 44: 42-46. DOI: 10.1002/(SICI)1521-3803(20000101) 44:1<42::AID-FOOD42>3.0.CO;2-D

    Roofchaee A, Mehrdad I, Ebrahimzadeh MA & Akbari MR. 2011. Effect of dietary Boregano (Origanum Vulgare L.) essential oil on growth performance, cecal microflora and serum antioxidant activity of broiler chickens. African Journal of Biotechnology, 32: 6177-6183.

    Rostami F, Ghasemi HA & Taherpour K. 2015. Effect of Scrophularia striata and Ferulago angulata, as alternatives to virginiamycin, on growth performance, intestinal microbial population, immune response, and blood constituents of broiler chickens. Poultry Science, 94: 2202-2209. DOI: 10.3382/ps/pev198

    Sahin K, Onderci M, Sahin N, Gursu MF & Kucuk O. 2003. Dietary vitamin C and folic acid supplementation ameliorates the detrimental effects of heat stress in Japanese quail. The Journal of Nutrition,  133:   1882  -  1886.   DOI:  10.1093/jn/

    133.6.1882

    Saleh N, Allam T, El-latif AA & Ghazy E. 2014. The effects of dietary supplementation of different levels of thyme (Thymus vulgaris) and ginger (Zingiber offcinale) essential oils on performance, hematological, biochemical and immunological parameters of broiler chickens. Global veterinaria, 6: 736-744. DOI: 10.5829/idosi.gv. 2014.12. 06.83189

    SAS, 2001. SAS User’s Guide: Statistics, Version 9.2, SAS Institute, North Carolina, USA.

    Si W, Gong J, Tsao R, Zhou T, Yu H, Poppe C, Johanson R & Du Z. 2006. Antimicrobial activity of essential oils and structurally related synthetic food additives towards selected pathogenic and beneficial gut bacteria. Journal of Applied Microbiology, 100: 269-305. DOI: 10.1111/j.1365-2672.2005.02789.x

    Smith-Palmer A, Stewart J & Fyfe L. 2004. Influence of sub-inhibitory concentrations of plant essential oils on the produc-tion of enterotoxins A and B and alpha toxin by Staphylococ-cus aureus. Journal of Medical Microbiology, 53: 1023-1027. DOI: 10.1099/jmm.0.45567-0

    Takahashi K, Mashiko T & Akiba Y. 2000. Effects of dietary concentration of xylitol on growth in male broiler chicks during immunological stress. Poultry Science, 79: 743-747. DOI: 10.1093/ps/79.5.743

    Ultee A, Kets EPW & Smid EJ. 1999. Mechanisms of action of carvacrol on the food borne pathogen Bacillus cereus. Applied and Environmental Microbiology, 65: 4606-4610. DOI:
    10.1128/AEM.65.10.4606-4610.1999

    Vidanarachchi JK, Mikkelsen LL, Sims I, Iji PA & Choct M. 2005. Phytobiotics: alternatives to antibiotic growth promoters in monogastric animal feeds. Recent Advances in Animal Nutrition in Australia, 15: 131-144.

    Wallace RJ, Oleszek W, Franz C, Hahn I, Baser KHC, Mathe A & Teichmann K. 2010. Dietary plant bioactive for poultry health and productivity. British Poultry Science, 51: 461-487. DOI: 10.1080/00071668.2010.506908

    Wawrzyniak A, Kapica M, Stępień-Pyśniak D, Łuszczewska-Sierakowska I, Szewerniak R & Jarosz Ł. 2017. The effect of dietary supplementation of transcarpathian zeolite on intestinal morphology in female broiler chickens. Journal of Applied Poultry Research, 26: 421-430. DOI: 10.3382/japr/pfx011

    Xu, ZR, Hu CH, Xia MS, Zhan XA & Wang MQ. 2003. Effects of dietary fructo-oligosaccharide on digestive enzyme activities, intestinal microflora and morphology of male broilers. Poultry Science, 82: 648–654. DOI: 10.1093/ps/82.6.1030

    Zamanian Azodi M, Ardashiri Lajimi A, Rezaei Tavirani M & Khodarahmi R. 2012. Antibiotic effect of aqueous extract of Scrophularia striata on E.coli in comparison with tetracycline. Scientific Journal of Ilam University 8(4): 127-133 (Persian).

    Zeng Z, Zhang S, Wang H & Paio X. 2015. Essential oil and aromatic plants as feed additives in non-ruminant nutrition: a review. Journal of Animal Science and Biotechnology, 6: 7-15. DOI: 10.1186/s40104-020-00467-w

Poultry Science Journal
Volume 9, Issue 2
December 2021
Pages 207-219

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