The Effect of Fermented Sesame Meal or its Combination with Probiotics on the Performance, Carcass Triats, Blood Parameters, and Humoral Immunity in Growing Japanese Quails

Document Type : Original Paper


Department of Animal Science, College of Agriculture, University of Zabol, Zabol, Iran


In this experiment, the effect of fermented sesame meal or its combination with probiotics was evaluated on performance, carcass traits, blood parameters, and humoral immunity of Japanese quails. In a completely randomized design, 480 seven-day-old Japanese quails were assigned to eight treatments, four replicates, and fifteen chicks per replicate. Treatments were corn-soybean meal diet as a basal or control group (1), and basal diet containing  35% of sesame meal (2), 35% of fermented sesame meal with Saccharomyces cerevisiae (3), 35% of sesame meal + Saccharomyces cerevisiae probiotic (4), 35% of fermented sesame meal with Bacillus subtilis (5), 35% of sesame meal + Bacillus subtilis probiotic (6) 35% of sesame fermented meal with Lactobacillus sakei (7), and 35%  of sesame meal + Lactobacillus sakei probiotic (8). The study results indicated that treatments with sesame meal, compared to control treatment, had no significant effects on the performance and blood parameters of Japanese quails. Birds fed with a diet containing 35% of fermented sesame meal with Saccharomyces cerevisiae had a lower relative liver weight than the control treatment, whereas birds fed with a diet containing 35% sesame meal plus Lactobacillus sakei probiotic had a greater relative liver weight (P < 0.05). Treatment with fermented sesame meal with Saccharomyces cerevisiae had a higher level of antibody produced against sheep red blood cells and Newcastle virus than those of the control group (P < 0.05). Findings revealed that up to 35% of sesame meal not only does not impair the performance of Japanese quails, and fermenting of this protein supply but can also improves the birds’ health.


Ahmadi MR, Farrokhi E, Agharokh B, Khiavi M, Arab G & Mohammadi E. 2000. Registration of Sesame (Sesamum indicum) cultivar, Yekta. Seed and Plant Journal, 16: 390-41.
Al Harthi M & El Deek A. 2009. Evaluation of sesame meal replacement in broiler diets with phytase and probiotic supplementation. Egypt Poultry Science Journal, 29: 99-125.
Alshelmani MI, Loh TC, Foo HL, Sazili AQ & Lau WH. 2016. Effect of feeding different levels of palm kernel cake fermented by Paenibacillus polymyxa ATCC 842 on nutrient digestibility, intestinal morphology, and gut microflora in broiler chickens. Animal Feed Science and Technology, 216: 216-224. DOI: 10.1016/ j.anifeedsci.2016.03.019
Bell D, Ibrahim A, Denton G, Long G & Bradley G. 1990. An evaluation of sesame seed meal as a possible substitute for soyabean oil meal for feeding broilers. Poultry Science, 69: 672-684. DOI: 10.3382/ps.0740672
Borchani C, Besbes S, Blecker C & Attia H. 2010. Chemical characteristics and oxidative stability of sesame seed, sesame paste, and olive oils. Journal of Agricultural Science and Technology, 12: 585-596.
Cheema M, Qureshi M & Havenstein G. 2003. A comparison of the immune response of a 2001 commercial broiler with a 1957 randombred broiler strain when fed representative 1957 and 2001 broiler diets. Poultry Science, 82: 1519-1529. DOI: 10.1093/ps/82.10.1519
Christensen HR, Frøkiær H & Pestka JJ. 2002. Lactobacilli differentially modulate expression of cytokines and maturation surface markers in murine dendritic cells. Journal of Immunology, 168: 171-178. DOI: 10.4049/jimmunol.168.1.171
Farran, MTG, Uwayjan AM & Ashkarian VM. 2000. Performance of broilers and layers fed graded level of sesame hull, Journal Applied Poultry Research, 9: 453-459. DOI: 10.1093/japr/9.4.453
FAO (Food and Agriculture Organization of the United Nations). 2019.  http://www.
Feng J, Liu X, Xu Z, Lu Y & Liu Y. 2007. The effect of Aspergillus oryzae fermented soybean meal on growth performance, digestibility of dietary components and activities of intestinal enzymes in weaned piglets. Animal Feed Science and Technology, 134: 295-303. DOI: 10.1016/ j.anifeedsci.2006.10.004
Ghazvinian K, Pour HA & Alanghi AR. 2017. Effect of sesame meal supplementation to the feed on performance, blood parameters and physiology characteristics in Japanese quail. Entomology and Applied Science Letters, 3: 71-75.
Hou Y, Wu Z, Dai Z, Wang G & Wu G. 2017. Protein hydrolysates in animal nutrition: Industrial production, bioactive peptides, and functional significance. Journal of Animal Science and Biotechnology, 8: 24. DOI: 10.1186/s40104-017-0153-9
Iwao M, Gotoh K, Arakawa M, Endo M, Honda K, Seike M, Murakami K & Shibata H. 2020. Supplementation of branched-chain amino acids decreases fat accumulation in the liver through intestinal microbiota-mediated production of acetic acid. Scientific Reports, 30: 10:1-11. DOI: 10.1038/s41598-020-75542-3
Kapila S & Sinha VP. 2006. Antioxidative and hypocholesterolemic effect of Lactobacillus casei ssp casei (biodefensive properties of lactobacilli). Indian Journal of Medical Sciences, 60: 361-370.  DOI: 10.4103/0019-5359.27220
Khempaka S, Pudpila U & Molee W. 2013. Effect of dried peppermint (Mentha cordifolia) on growth performance, nutrient digestibility, carcass traits, antioxidant properties, and ammonia production in broilers. Journal of Applied Poultry Research, 22: 904-912. DOI: 10.3382/japr.2013-00813
Kindeya Y, Firew M & Eyasu A. 2019. Evaluation and registration of white seeded sesame variety (Sesamum indicum L.): Setit-3 (Hurc-4) in western Tigray, Ethiopia" Describes the performance of a new sesame variety in western Tigray in Ethiopia. Journal of Research in Agriculture and Forestry, 6: 23-28.
Mamputu M & Buhr R. 1995. Effect of substituting sesame meal for soybean meal on layer and broiler performance. Poultry Science, 74: 672-684. DOI: 10.3382/ps.0740672
Ngele G, Oyawoye E & Doma U. 2011. Performance of broiler chickens fed raw and toasted sesame seed (sesanum indicum, l) as a source of methionine Continental. Journal of Agricultural Science, 5: 33-38. DOI: 10.5281/zenodo.839959
Niba A, Beal J, Kudi A & Brooks P. 2009. Potential of bacterial fermentation as a biosafe method of improving feeds for pigs and poultry. African Journal of Biotechnology, 8: 1758-1768.
NRC. 1994. Nutrient Requirements for Poultry. 9th rev. ed. National Academy Press, Washington, DC.
Ooi LG & Liong MT. 2010. Cholesterol-lowering effects of probiotics and prebiotics: a review of in vivo and in vitro findings. International Journal of Molecular Sciences, 11: 2499-2522. DOI: 10.3390/ijms11062499
 Panda A, Reddy M, Ramarao S & Praharaj N. 2000. Effect of dietary supplementation of probiotic on performance and immune response of layers in the decline phase of production. Indian Journal of Poultry Science, 35: 102-104.
Park SH, Ryu SN, Bu Y, Kim H, Simon JE & Kim KS. 2010. Antioxidant components as potential neuroprotective agents in sesame (Sesamum indicum L.). Food reviews international, 26: 103-121. DOI: 10.1080/87559120903564464
Parvez S, Malik KA, Ah Kang S & Kim HY. 2006. Probiotics and their fermented food products are beneficial for health. Journal of Applied Microbiology, 100: 1171-1185. DOI: 10.1111/ j.1365-2672.2006.02963.x
Santoso U, Tanaka K & Ohtani S. 1995. Effect of dried Bacillus subtilis culture on growth, body composition and hepatic lipogenic enzyme activity in female broiler chicks. British Journal of Nutrition, 74: 523-529. DOI: 10.1079/ BJN19950155
SAS. 2002. SAS/STAT 9.1 User's Guide SAS Institute Inc, Cary, NC.
Shi C, Zhang Y, Lu Z & Wang Y. 2017. Solid-state fermentation of corn-soybean meal mixed feed with Bacillus subtilis and Enterococcus faecium for degrading antinutritional factors and enhancing nutritional value. Journal of Animal science and Biotechnology, 8: 1-9. DOI: 10.1186/s40104-017-0184-2
Song YS, Frías J, Martinez-Villaluenga C, Vidal-Valdeverde C & de Mejia EG. 2008. Immunoreactivity reduction of soybean meal by fermentation, effect on amino acid composition and antigenicity of commercial soy. Food Chemistry, 108: 571-581. DOI: 10.1016/ j.foodchem.2007.11.013
Sugiharto S & Ranjitkar S. 2019. Recent advances in fermented feeds towards improved broiler chicken performance, gastrointestinal tract microecology and immune responses: A review. Animal Nutrition,5: 1-10.  DOI: 10.1016/j.aninu.2018. 11.001
Vioque J, Sánchez-Vioque R, Clemente A, Pedroche J & Millán F. 2000. Partially hydrolyzed rapeseed protein isolates with improved functional properties. Journal of the American Oil Chemists' Society, 77: 447-50. DOI: 10.1007/s11746-000-0072-y.
Xie PJ, Huang LX, Zhang CH & Zhang YL. 2016. Nutrient assessment of olive leaf residues processed by solid‐state fermentation as an innovative feedstuff additive. Journal of Applied Microbiology, 121: 28-40. DOI: 10.1111/ jam.13131
Yakubu B & Alfred B. 2014. Nutritional Evaluation of Toasted White Sesame Seed Meal Sesamum Indicum as A Source of Methionine on Growth Performance, Carcass Characteristics, Haematological and Biochemical Indices of Finisher Broiler Chickens. Journal of Agriculture and Veterinary Science, 7: 46-52. DOI: 10.9790/ 2380-07124652
Yalçın S, Eser H, Yalçın S, Cengiz S & Eltan Ö. 2013. Effects of dietary yeast autolysate (Saccharomyces cerevisiae) on performance, carcass and gut characteristics, blood profile, and antibody production to sheep red blood cells in broilers. The Journal of Applied Poultry Research, 22: 55-61. DOI: 10.3382/japr.2012-00577
Yusrizal Y & Chen T. 2003. Effect of adding chicory fructans in feed on fecal and intestinal microflora and excreta volatile ammonia. International Journal of Poultry Science, 2: 188-194. DOI: 10.3923/igps.2003.188.194.