Effect of Adding Watermelon Juice, Vitamin E-Selenium, and Vitamin C into the Drinking Water on Growth Performance, Internal Organs’ weight and Prececal Nutrient Digestibility in Broilers under Heat Stress

Document Type : Original Paper


Department of Animal Science, School of Agriculture, Shiraz University, Bajgah, Shiraz, Iran


This study investigated the effect of adding watermelon juice, vitamin E selenium and vitamin C to the drinking water on growth performance, internal organs’ weight, and prececal nutrient digestibility in broilers under heat stress. Two hundred and fifty Hubbard F15 day-old broilers were allocated to five treatments with five replicates of 10 broilers per each replicate, based on a completely randomized design. The birds in the control treatment did not receive any supplement in drinking water and reared in thermoneutral temperature. Birds in the other four treatments received watermelon juice at 60% or 30%, vitamin C at 50 mg/L, or vitamin E-Selenium at 1 mL/L of drinking water during the heat stress period. Heat stress applied for 9 hours from day 12 onward. Results indicated birds that received 60% watermelon juice had similar body weight and feed intake compared to control birds. The relative weight of proventriculus, intestine length, and prececal dry matter nutrient digestibility was significantly higher in 60% watermelon juice treatment than other treatments. It is concluded that watermelon juice supplementation at 60% concentration into the drinking water could improve the performance of birds reared under heat stress as a result of restoring water and feed intake and also better prececal dry matter digestibility.


Ait-Boulahsen A, Garlich J & Edens F. 1992. Relationship between blood ionized calcium and body temperature of chickens during acute heat stress. Proc 19th World’s Poult Cong, 20-24.
Ait-Boulahsen A, Garlich JD & Edens FW. 1995. Potassium chloride improves the thermotolerance of chickens exposed to acute heat stress. Poultry Science, 74: 75-87. DOI: 10.3382/ps.0740075
Allen PC. 1997. Production of free radical species during Eimeria maxima infections in chickens. Poultry Science, 76: 814-821. DOI: 10.1093/ps/ 76.6.814
AOAC International. 2006. AOAC International guidelines for laboratories performing microbiological and chemical analyses of food and pharmaceuticals: An aid to interpretation of ISO/IEC 17025: 2005 AOAC International.
Belay T & Teeter R. 1993. Broiler water balance and thermobalance during thermoneutral and high ambient temperature exposure. Poultry Science, 72: 116-124. DOI: 10.3382/ps.0720116
Bonnet S, Geraert P, Lessire M, Carre B & Guillaumin S. 1997. Effect of high ambient temperature on feed digestibility in broilers. Poultry Science, 76: 857-863. DOI: 10.1093/ps/ 76.6.857
Borges S, Fischer da Silva A, Majorka A, Hooge D & Cummings K. 2004. Physiological responses of broiler chickens to heat stress and dietary electrolyte balance (sodium plus potassium minus chloride, milliequivalents per kilogram). Poultry Science, 83: 1551-1558. DOI: 10.1093/ps/ 83.9.1551
Bottje W, Erf G, Bersi T, Wang S, Barnes D & Beers K. 1997. Effect of dietary dl-alpha-tocopherol on tissue alpha-and gamma-tocopherol and pulmonary hypertension syndrome (ascites) in broilers. Poultry Science, 76: 1506-1512. DOI: 10.1093/ps/76.11.1506
Bottje W & Harrison P. 1985. Effect of carbonated water on growth performance of cockerels subjected to constant and cyclic heat stress temperatures. Poultry Science, 64: 1285-1292. DOI: 10.3382/ps.0641285
Bottje WG & Wideman R. 1995. Potential role of free radicals in the pathogenesis of pulmonary hypertension syndrome. Poultry and Avian Biology Reviews, 6: 211-231.
Branton S, Reece F & Deaton J. 1986. Use of ammonium chloride and sodium bicarbonate in acute heat exposure of broilers. Poultry Science, 65: 1659-1663. DOI: 10.3382/ps.0651659
Cooper M & Washburn K. 1998. The relationships of body temperature to weight gain, feed consumption, and feed utilization in broilers under heat stress. Poultry Science, 77: 237-242. DOI: 10.1093/ps/77.2.237
De Basilio V, Vilarino M, Yahav S & Picard M. 2001. Early age thermal conditioning and a dual feeding program for male broilers challenged by heat stress. Poultry Science, 80: 29-36. DOI: 10.1093/ps/80.1.29
De Coca-Sinova A, Mateos GG, González-Alvarado J, Centeno C, Lázaro R & Jiménez-Moreno E. 2011. Comparative study of two analytical procedures for the determination of acid insoluble ash for evaluation of nutrient retention in broilers. Spanish Journal of Agricultural Research, 9: 761-768.
Deyhim F & Teeter R. 1991. Research note: sodium and potassium chloride drinking water supplementation effects on acid-base balance and plasma corticosterone in broilers reared in thermoneutral and heat-distressed environments. Poultry Science, 70: 2551-2553. DOI: 10.3382/ps. 0702551
Erf G & Bottje W. 1996. Nutrition and immune function in chickens: Benefits of dietary vitamin E supplementation. Proceedings of the Arkansas Nutrition Conference, Fayetteville, AR.
Figueroa A, Sanchez-Gonzalez MA, Perkins-Veazie PM & Arjmandi BH. 2011. Effects of watermelon supplementation on aortic blood pressure and wave reflection in individuals with prehypertension: a pilot study. American Journal of Hypertension, 24: 40-44. DOI: 10.1038/ajh. 2010.142
Habibian M, Ghazi S, Moeini MM & Abdolmohammadi A. 2014. Effects of dietary selenium and vitamin E on immune response and biological blood parameters of broilers reared under thermoneutral or heat stress conditions. International Journal of Biometeorology, 58: 741–752. DOI: 10.1007/s00484-013-0654-y
Holden JM, Eldridge AL, Beecher GR, Buzzard IM, Bhagwat S, Davis CS, Schakel, S. 1999. Carotenoid content of US foods: an update of the database Journal of Food Composition and Analysis, 12: 169-196. DOI: 10.1006/jfca. 1999.0827
Hurwitz S, Weiselberg M, Eisner U, Bartov I, Riesenfeld G, Sharvit M, Bornstein S. 1980. The energy requirements and performance of growing chickens and turkeys as affected by environmental temperature Poultry Science, 59: 2290-2299. DOI: 10.3382/ps.0592290
Hubbard F15 Manual 2015 Broiler performance and nutrition supplement page 41, www.hubbardbreeders.com
Huston TM. 1978. The effect of environmental temperature on potassium concentrations in the blood of the domestic fowl. Poultry Science, 57: 54-56. DOI: 10.3382/ps.0570054
Jiang W, Zhang L & Shan A. 2013. The effect of vitamin E on laying performance and egg quality in laying hens fed corn dried distillers grains with solubles. Poultry Science, 92: 2956–2964. DOI: 10.3382/ps.2013-03228
Ko SH, Choi SW, Ye SK, Cho BL, Kim HS & Chung MH. 2005. Comparison of the antioxidant activities of nine different fruits in human plasma. Journal of medicinal food, 8: 41-46. DOI: 10.1089/jmf.2005.8.41
Kutlu H & Forbes J. 1993. Changes in growth and blood parameters in heat-stressed broiler chicks in response to dietary ascorbic acid. Livestock Production Science, 36: 335-350. DOI: 10.1016/ 0301-6226(93)90050-R
Leeson S & Summers JD. 2001. Scotts Nutrition of the Chicken (4nd ed) University Books, Canada NA.
Lepkovsky S, Chari-Bitron A, Lyman R & Dimick M. 1960 Food intake, water intake and body water regulation. Poultry Science, 39: 390-394. DOI: 10.3382/ps.0390390
May J & Lott B. 1992. Feed and water consumption patterns of broilers at high environmental temperatures. Poultry Science, 71: 331-336. DOI: 10.3382/ps.0710331
McKee J & Harrison P. 1995. Effects of supplemental ascorbic acid on the performance of broiler chickens exposed to multiple concurrent stressors. Poultry Science, 74: 1772-1785. DOI: 10.3382/ps. 0741772
Mohammed AA, Jiang S, Jacobs JA & Cheng HW. 2019. Effect of a synbiotic supplement on cecal microbial ecology, antioxidant status, and immune response of broiler chickens reared under heat stress. Poultry Science, 98: 4408-4415. DOI: 10.3382/ps/pez246
Nockels CF. 1973. The influence of feeding ascorbic acid and sulfate on egg production and on cholesterol content of certain tissues of the hen. Poultry Science, 52: 373-378. DOI: 10.3382/ps. 0520373
Pardue S & Thaxton JP. 1986. Ascorbic acid in poultry: a review. World's Poultry Science Journal, 42: 107-123. DOI: 10.1079/ WPS 19860009
Ross Breeder Limited. 1999. Broiler management manual Technical Services Department, Newbridge, Midlothian, Scotland, UK
SAS (Statistical Packages for the Social Sciences). 2013. SAS/STAT® 9.4. User’s Guide. SAS Institute Inc. Cary, North Carolina.
Scott, TA & Hall, JW. 1998. Using acid insoluble ash marker ratios (diet:digesta) to predict digestibility of  wheat  and  barley  metabolizable   energy  and nitrogen retention in broiler chicks, Poultry Science, 77: 674-679, DOI: 10.1093/ps/77.5.674
Shakeri M, Oskoueian E, Le HH & Shakeri M. 2020. Strategies to combat heat stress in broiler chickens: unveiling the roles of selenium, vitamin E and vitamin C Veterinary Sciences, 7: 1-9. DOI: 10.3390/vetsci7020071
Smith M & Teeter R. 1987. Potassium balance of the 5 to 8-week-old broiler exposed to constant heat or cycling high temperature stress and the effects of supplemental potassium chloride on body weight gain and feed efficiency. Poultry Science, 66: 487-492. DOI: 10.3382/ps.0660487
Sogi D, Oberoi D & Malik S. 2010. Effect of particle size, temperature, and total soluble solids on the rheological properties of watermelon juice: A response surface approach. International Journal of Food Properties, 13: 1207-1214. DOI: 10.1080/10942910903013373
Tarazona‐Díaz MP, Viegas J, Moldao‐Martins M & Aguayo E. 2011. Bioactive compounds from flesh and by‐product of fresh‐cut watermelon cultivars. Journal of the Science of Food and Agriculture, 91: 805-812. DOI: 10.1002/jsfa.4250
Teeter R & Smith M. 1986. High chronic ambient temperature stress effects on broiler acid-base balance and their response to supplemental ammonium chloride, potassium chloride, and potassium carbonate. Poultry Science, 65: 1777-1781. DOI: 10.3382/ps.0651777
United States Department of Agriculture. 2018. USDA Food Composition Databases. Retrieved from http://ndb.nal.usda.gov/ndb/foods/show/ 301290?manu=&fgcd=&ds=SR&q=Watermelon,%20 raw
van Niekerk T, Garber T, Dunnington E, Gross W & Siegel P. 1989. Response of white leghorn chicks fed ascorbic acid and challenged with Escherichia coli or with corticosterone. Poultry Science, 68: 1631-1636. DOI:  10.3382/ps.0681631
Zhang ZY, Jia GQ, Zuo JJ, Zhang Y, Lei J, Ren L & Feng D. 2012. Effects of constant and cyclic heat stress on muscle metabolism and meat quality of broiler breast fillet and thigh meat. Poultry Science, 91: 2931-2937. DOI: 10.3382/ps.2012-02255.