Influence of Dietary Supplementation of Kigelia pinnata and Plukenetia conophora Leaves on Cytokine Expression, Immunoglobulins, Blood Chemistry, Caecal Microbiota and Meat Quality in Broiler Chickens

Document Type : Original Paper

Authors

Department of Animal Production, Faculty of Agriculture, University of Ilorin, PMB 1515 Ilorin, Nigeria

Abstract

This study examined the effect of dietary supplementation of Kigelia pinnata (KPL) and Plukenetia conophora (PCL) leaves in comparison with oxytetracycline (OXY) and butylated hydroxyanisole (BHA) on growth performance, selected blood biochemical parameters, caecal microbiota, splenic interleukins (IL), serum immunoglobulins (Ig), carcass traits, meat quality, and oxidative stability in broiler chickens. One day old Arbor Acres chicks (n=420) were randomly assigned to either basal diet only (BD); basal diet + 0.5 g/kg oxytetracycline + 0.12 g/kg BHA (OXYBHA); basal diet + 1 g/kg KPL (KPL-1); basal diet + 2 g/kg KPL (KPL-2); basal diet + 1 g/kg PCL (PCL-1); or basal diet + 2 g/kg PCL (PCL-2) for 42 d. Each dietary treatment had seven replicates with 10 chicks per replicate. Supplemented birds gained (P < 0.05) more weight and had a better feed conversion ratio compared with the BD birds. Hematological indices, IL-1β, and IL-6 did not differ among the treatments. BD birds had lower (P < 0.05) carcass weight and IL-10, and higher (P < 0.05) IgG, IgM, Salmonella spp., and E. coli counts than the supplemented birds. The KPL-2 birds had the least (P < 0.05) E. coli and Salmonella spp. counts and IgM among the supplemented birds. Lactobacillus spp. count was lower (P < 0.05) in OXYBHA birds compared with KPL and PCL birds. Carbonyl and malondialdehyde contents in the Sartorius muscle, and drip loss and carbonyl content in the Pectoralis muscle of the BD birds were higher (P < 0.05) than those of the supplemented birds. These results illustrate that the 2 g/kg KPL and 2 g/kg PCL could be used as an antioxidant and an antimicrobial in the diets of broiler chickens.

Keywords

References

Abu Bakar MF, Mohamed M, Rahmat A & Fry J. 2009. Phytochemicals and antioxidant activity of different parts of bambangan (Mangifera pajang) and tarap (Artocarpus odoratissimus). Food Chemistry, 113: 479-483. DOI: 10.1016/ j. food chem.2008.07.081
Adeyemi KD. 2021. Comparative effect of dietary Morinda lucida leaf and Butylated hydroxyanisole (BHA) on carcass traits, meat quality, and oxidative stability of broiler chickens. Journal of Food Science and Technology, DOI: 10.1007/ s13197-020-04916-2
Adeyemi KD, Abdulrahman A, Ibrahim SO, Zubair MF, Atolani O & Badmos AA. 2020. Dietary Supplementation of Tetracarpidium conophorum (African Walnut) seed enhances muscle n‐3 fatty acids in broiler chickens. European Journal of Lipid Science and Technology, 122: 1900418. DOI: 10.1002/ejlt.201900418
Adeyemi KD, Shittu RM, Sabow AB, Ebrahimi M, Sazili AQ. 2016. Influence of diet and postmortem ageing on oxidative stability of lipids, myoglobin and myofibrillar proteins and quality attributes of gluteus medius muscle in goats. PloS one, 11: e0154603. DOI: 10.1371/journal. pone. 0154603
Ajaiyeoba EO & Fadare DA. 2006. Antimicrobial potential of extracts and fractions of the African walnut–Tetracarpidium conophorum. African Journal of Biotechnology, 5: 2322-2325.
Ansari J, Khan SH, Haq AU, Ahmad T & Abbass MI. 2013. Effect of Supplementation of Withania somnifera (Linn.) Dunal Roots on Growth Performance, Serum Biochemistry, Blood Hematology, and Immunity of Broiler Chicks. Journal of herbs, Spices and Medicinal Plants, 19: 144-158. DOI: 10.1080/10496475.2012.759169
Association of Official Analytical Chemists (AOAC) (2000) ‘Official methods of analysis. Vol I.’ 17th edn. (AOAC International Gaithersburg, MD).
Baurhoo B, Phillip L & Ruiz-Feria CA. 2007. Effects of purified lignin and mannan oligosaccharides on intestinal integrity and microbial populations in the ceca and litter of broiler chickens. Poultry Science, 86: 1070-1078. DOI: 10.1093/ps/ 86.6.1070
Bors W, Heller W, Michael C & Stettmaier K. 1996. Flavonoids and polyphenols: Chemistry and biology in: Handbook of Antioxidants, Cadenas E and Packer L (eds), New York, Marcel Dekker 409–66.
Buege JA & Aust SD. 1978. Microsomal lipid peroxidation. In Methods in enzymology. 52: 302-310. Academic Press, New York.
Commission International De I’ Eclairage (CIE) (1976) ‘Colorimetry.’ 2nd edn. (CIE: Vienna, Switzerland).
Crowell PL. 1999. Prevention and therapy of cancer by dietary monoterpenes. Journal of Nutrition, 129: 775S-778S. DOI: 10.1093/jn/129.3.775S
da Rosa JS, Nascimento MVPDS, Parisotto EB, Lima TC, Santin JR, Biavatti MW, Zamoner A, Dalmarco EM & Fröde TS. 2019. Phenolic Compounds Isolated from Calea uniflora Less. Promote Anti-Inflammatory and Antioxidant Effects in Mice Neutrophils (Ex Vivo) and in Mice Pleurisy Model (In Vivo). Mediators of Inflammation, 1468502. DOI: 10.1155/2019/ 1468502
Dalal R, Ahlawat PK, Sonu V, Panwar VS, Tewatia BS & Sheoran N. 2018. Evaluation of Antimicrobial Effect of Emblica officinalis Fruit Powder on Intestinal Micro-biota in Broilers Chicken. International Journal of Current Microbiology and Applied Science, 7: 1432-1438. DOI: 10.20546/ijcmas.2018.704.162
Du B, Zhang Y, Lam JC, Pan S, Huang Y, Chen B, & Zeng L. 2019. Prevalence, biotransformation, and maternal transfer of synthetic phenolic antioxidants in pregnant women from South China. Environmental Science and Technology, 53:13959-13969. DOI: 10.1021/acs.est.9b04709
Edwards EA & Hilderbrand RL. 1976. Method for identifying Salmonella and Shigella directly from the primary isolation plate by coagglutination of protein A-containing staphylococci sensitized with specific antibody. Journal of Clinical Microbiology, 3: 339–343.
EFSA. 2016. The European Union summary report on antimicrobial resistance in zoonotic and indicator bacteria from humans, animals and food in 2014. European Food Safety Authority Journal, 4380.
FDA. 2013. Phasing out certain antibiotic use in farm animals. http://www.fda.gov/ For Consumers/ Consumer Updates /ucm378100.htm. 2013. Accessed June 2020.
Giansanti F, Giardi MF & Botti D. 2006. Avian cytokines-An overview. Current Pharmaceutical Design, 12: 3083-3099. DOI: 10.2174/ 138161206777947542
Gouda YG, Abdel-Baky AM, Mohamed KM, Darwish FM, Kasai R & Yamasaki K. 2006. Phenylpropanoid and phenylethanoid derivatives from Kigelia pinnata DC fruits. National Production Research, 20: 935-939. DOI: 10.1080/ 14786410500462702
Hashemi SR & Davoodi H. 2011. Herbal plants and their derivatives as growth and health promoters in animal nutrition. Veterinary Research Communications, 35: 169-180. DOI: 10.1007/ s11259-010-9458-2
Hussain T, Fatima I, Rafay M, Shabir S, Akram M & Bano S. 2016. Evaluation of antibacterial and antioxidant activity of leaves, fruit and bark of Kigelia africana. Pakistan Journal of Botany, 48: 277-83.
Iqbal Z, Kamran Z, Sultan JI, Ali A, Ahmad S, Shahzad MI, Ahsan U, Ashraf S & Sohail MU. 2015. Replacement effect of vitamin E with grape polyphenols on antioxidant status, immune, and organs histopathological responses in broilers from 1-to 35-d age. Journal of Applied Poultry Research, 24: 127-134. DOI: 10.3382/japr/pfv009
Kaiser P & Stäheli P. 2014. Avian cytokines and chemokines. In Avian immunology. Academic Press. Pages, 189-204.
Kogut MH. 2019. The effect of microbiome modulation on the intestinal health of poultry. Animal Feed Science and Technology, 250: 32-40.
Kogut MH, Lee A & Santin E. 2020. Microbiome and pathogen interaction with the immune system. Poultry Science, 99: 1906-1913. DOI: 10.1016/ j.psj.2019.12.011
Kohl KD. 2012. Diversity and function of the avian gut microbiota. Journal of Comparative Physiology B, 182: 591-602. DOI: 10.1007/ s00360-012-0645-z
Levine RL, Garland D & Oliver CN. 1990. Determination of carbonyl content in oxidatively modified proteins. Method in Enzymology, 186: 464-478.
Lye HS, Rusul G & Liong MT. 2010. Removal of cholesterol by lactobacilli via incorporation and conversion tocoprostanol. Journal of Dairy Science, 93: 1383–1392. DOI: 10.3168/jds.2009-2574
Maduka HCC, Ugwu CE, Okpogba AN, Ogueche PN, Dike CC, Okonkwo CO & Nwanyanwu AC. 2018. Phytochemical analysis and antioxidant properties of the ethanolic extract from Tetracarpidium conophorum (African Walnut) and Pterocarpus soyauxii (oha) Leaf. Journal of Applied Life Science International, 18: 1-7. DOI: 10.9734/JALSI/2018/42439
Makkar HPS, Norsambuu T, Lkhavatsere S & Becker K. 2009. Plant Secondary metabolites in some medical plants of Mongolia used for enhancing animal health and production. Tropicultura 27: 159-167.
Manzetti S & Ghisi R. 2014. The environmental release and fate of antibiotics. Marine Pollution Bulletion 79: 7-15. DOI: 10.1016/j.marpolbul. 2014.01.005
Mast J, Buyse J & Goddeeris BM. 2000. Dietary L-carnitine supplementation increases antigen-specific immunoglobulin G production in broiler chickens. British Journal of Nutrition, 83: 161-166. DOI: 10.1017/S0007114500000209
Mitruka BM & Rawsley HM. 1977. Clinical Biochemistry and Haematological Reference Value in Normal Experimental Animal. Mason Publishing Company New York. pp. 35-50.
NAFDAC. 2017. Antimicrobial use and resistance in Nigeria. Situation analysis and recommendations. Federal Ministries of Agriculture and Rural Development, Environment and Health Report.
Nakyinsige K, Abdul Rahman NS, Salwani MS, Abd Hamid A, Adeyemi KD, Sakimin SZ & Sazili A. 2016. Effect of Belimbing buluh (Averrhoa bilimbi) juice extract on oxidative stability and microbiological quality of spent chicken meat. International Food Research Journal, 23: 2657-2680.
Nodu MB, Okpeku M, Akpoveta ZA & Iroegbu DO. 2016. Evaluation of azadirachta indica leave extract on hematology and biochemical profiles, organs weight and growth parameters of broiler chickens. Journal of New Science, 32: 5
NRC (National Research Council). 1994. Nutrient Requirements of Poultry. 9th ed. Washington: National Academy Press.
Odebiyi OO & Sofowora EA. 1978. Phytochemical screening of Nigerian medicinal plants II. Lloydia, 41: 234-246.
Odhaib, K.J, Adeyemi KD, Ahmed MA, Jahromi MF, Jusoh S, Samsudin AA, Alimon AR, Yakoob H & Sazili, AQ. 2018a. Influence of Nigella sativa seeds, Rosmarinus officinalis leaves and their combination on growth performance, immune response and rumen metabolism in Dorper lambs. Tropical Animal Health and Production, 50: 1011-1023. DOI: 10.1007/s11250-018-1525-7
Odhaib KJ, Adeyemi KD & Sazili AQ. 2018b. Carcass traits, fatty acid composition, gene expression, oxidative stability and quality attributes of different muscles in Dorper lambs fed Nigella sativa seeds, Rosmarinus officinalis leaves and their combination. Asian-Australasian Journal of Animal Science, 31:1345-1357. DOI: 10.5713/ajas.17.0468
Olorunsanya AO, Adeyemi KD & Babatunde IA. 2012. Effect of Bamboo (Bambusa valgaris) and Elephant grass (Pennisetum purpureum) leaf extracts on oxidative stability of cooked and raw broiler meat. Journal of Agricultural Research and Development,10: 1-10.
Oloruntola OD, Ayodele SO, Adeyeye SA, Jimoh AO, Oloruntola DA & Omoniyi IS. 2019. Pawpaw leaf and seed meals composite mix dietary supplementation: effects on broiler chicken’s performance, caecum microflora and blood analysis. Agroforestry Systems, 94: 555-564. DOI: 10.1007/s10457-019-00424-1
Oviedo-Rondón EO. 2019. Holistic view of intestinal health in poultry. Animal Feed Science and Technology, 250: 1-8. DOI: 10.1016/j.anifeedsci. 2019.01.009
Oyekale KO, Odutayo OI, Esan EB, Ogunwemimo KO, Denton OA & Bolaji DT. 2015. Comparative studies on phytochemical and proximate composition of four morphologically distinct segments of the conophor seedling (Tetracarpidium conophorum Hutch. & Dalziel). Brazilian Journal of Biological Science, 2: 91-100.
Pokorný J. 2007. Are natural antioxidants better–and safer–than synthetic antioxidants? European Journal of Lipid Science and Technology, 109: 629-642.
Ramasamy K, Abdullah N, Wong MC, Karuthan C & Ho YW. 2010. Bile salt deconjugation and cholesterol removal from media by Lactobacillus strains used as probiotics in chickens. Journal of the Science of Food and Agriculture, 90: 65-69. DOI: 10.1002/jsfa.3780
Salwani MS, Adeyemi KD, Sarah SA, Vejayan J, Zulkifli I & Sazili AQ. 2016. Skeletal muscle proteome and meat quality of broiler chickens subjected to gas stunning prior slaughter or slaughtered without stunning. CyTA Journal of Food, 14: 375-381. DOI: 10.1080/19476337. 2015.1112838
Serpen A, Gökmen V & Fogliano V. 2012. Total antioxidant capacities of raw and cooked meats. Meat Science, 90: 60-65. DOI: 10.1016/ j.meatsci. 2011.05.027
Shang Y, Kumar S, Oakley B & Kim WK. 2018. Chicken gut microbiota: importance and detection technology. Frontiers in Veterinary Science, 5: 254. DOI: 10.3389%2Ffvets.2018.00254
Sivagami K, Vignesh VJ, Srinivasan R, Divyapriya G & Nambi IM. 2020. Antibiotic usage, residues and resistance genes from food animals to human and environment: An Indian scenario. Journal of Environmental and Chemical Engineering, 8: 102221. DOI: 10.1016/j.jece.2018.02.029
Su JL, Shi BL, Zhang PF, Sun DS, Li TY & Yan SM. 2016. Effects of yucca extract on feed efficiency, immune and antioxidative functions in broilers. Brazilian Archive of Biology and Technology 59: e16150035. DOI: 10.1590/1678-4324-20161 50035
Swain T. 1979. Tannins and Lignins. In G.A Rosenthal and D.H. Janzen (eds.). Herbivores: Their interactions with plant secondary metabolites. Pp 657-682. Academic Press, New York.  
Talari S, Rudroju S, Penchala S & Nanna Rama S. 2012 Quantification of total phenolics and Total Flavonoid contents in extracts of Oroxylum Indicum I. Kurz. Asian Journal of Pharmaceutical and Clinical Research, 5: 177-179
Tao L. 2015. Oxidation of polyunsaturated fatty acids and its impact on food quality and human health. Advances in Food Technology and Nutrition Science Open Journal, 1: 135-42. DOI: 10.17140/ AFTNSOJ-1-123
Tietz NW. 1995. Fundamentals of Clinical Chemistry. 3rd edn, WB Saunders Philladelphia, Pages, 878.
Tolkachev ON, Shemeryakin BV & Prolina NV. 1983. Isolation and purification of alkaloids. Chemistry of Natural Compounds, 19: 387-400.
Van Boeckel TP, Brower C, Gilbert M, Grenfell BT, Levin SA, Robinson TP, Teilant A & Laxminarayan R. 2015. Global trends in antimicrobial use in food animals. Proceedings of National Academy of Science, 112: 5649-5654. DOI: 10.1073/pnas.1503141112
Van TTH, Yidana Z, Smooker PM & Coloe PJ. 2020. Antibiotic use in food animals worldwide, with a focus on Africa: Pluses and minuses. Journal of Global Antimicrobial Research, 20: 170-177. DOI: 10.1016/j.jgar.2019.07.031
Vanessa MS, Raphaël KJ & Kissel NN. 2019. Blood and Gut Micriobiota Profiles of Broiler Chickens Fed on Diet Supplemented with Graded Levels of Neem (Azadirachta indica) Oil. Animal Veterinary Science 7: 78-82. DOI: 10.11648/ j.avs.20190703.12
Vidanarachchi JK, Mikkelsen LL, Sims I, Iji PA & Choct M. 2005. Phytobiotics: alternatives to antibiotic growth promoters in monogastric animal feeds. Recent Advances in Animal Nutrition in Australia, 15: 131-44.
Wigley P, & Kaiser P. 2003. Avian cytokines in health and diseases. Brazilian Journal of Poultry Science, 5: 1-14.
Yang X, Sun Z, Wang W, Zhou Q, Shi G, Wei F & Jiang G. 2018. Developmental toxicity of synthetic phenolic antioxidants to the early life stage of zebrafish. Science of Total Environment, 643: 559-568. DOI: 10.1016/j.scitotenv. 2018. 06.213
Yusuf AL, Adeyemi KD, Roselina K, Alimon AR, Goh YM, Samsudin AA & Sazili AQ. 2018. Dietary supplementation of different parts of Andrographis paniculata affects the fatty acids, lipid oxidation, microbiota, and quality attributes of longissimus muscle in goats. Food Research International, 111: 699-707. DOI: 10.1016/ j. foodres.2018.06.015
Yuying M, Shihong Z, Minru, Jia, Guihua J, Shengwu T, Zhengyou H, Rong L. 2005. Determination of total coumarin in Angelica dahurica by UV spectrophotometry. West China Journal of Pharmaceutical Sciences, 20: 159-160.
Poultry Science Journal
Volume 9, Issue 1
June 2021
Pages 27-39

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