Effects of Pellet and Mash Diets of Mesquite Fruit (Prosopis juliflora) on Performance, Energy and Protein Efficiency Ratio and Intestinal Morphology of Broiler Chickens

Document Type : Original Paper


1 Department of Animal Science, Islamic Azad University, Arak Branch, Arak, Iran.

2 Animal Science Research Institute, Agricultural Research, Education, and Extension Organization, Karaj, Iran.


The primary aim of this study was to assay the influence of pellet and mash diets supplemented with different levels of mesquite fruit on performance, energy and protein efficiency ratio, carcass characteristics, and intestinal morphology of broiler chickens. In this study, a total of 560 one-day-old (mixed sex) Ross 308 were distributed in a completely randomized design with 7 treatments, 4 replicates, and 20 chicks per replicate. Seven iso-caloric and iso-nitrogenous diets including a control diet (mash form without mesquite fruit), and pelleted and mash diets, consisting of three levels of mesquite fruit (3, 6, and 9%) were adjusted. The results exhibited that mesquite fruit contained a high percentage of dietary fiber. The results also showed that pelleted and mash diets containing different levels of mesquite caused a significant difference in feed intake, body weight gain, and feed conversion ratio in starter and grower periods. At the finisher period, there was a significant difference between the experimental treatments in terms of feed intake and body weight gain, but there was no significant difference in feed conversion ratio. Experimental treatments also caused a significant difference in energy and protein efficiency ratio in the starter and grower periods. Dietary treatments also exhibited a significant difference in the relative weight of breast and intestine, but no effect was found on carcass yield and relative weight of other internal organs. The findings revealed that pelleted and mash diets containing different levels of mesquite fruit did not show significant differences in morphological traits of the intestine. Taken together, our results suggested that, the use of mesquite fruit in pelleted diets can have positive effects on the performance of broilers.


Abdollahi MR, Ravindran V & Svihus B. 2013. Pelleting of broiler diets an overview with emphasis on pellet quality and nutritional value. Animal Feed Science and Technology, 179: 1-23. DOI: 10.1016/j.anifeedsci.2012.10.011
Abdollahi MR, Ravindran V, Wester TJ, Ravindran G & Thomas DV. 2011. Influence of feed form and conditioning temperature on performance, apparent metabolisable energy and ileal digestibility of starch and nitrogen in broiler starters fed wheat-based diet. Animal Feed Science and Technology, 168: 88-99. DOI: 10.1016/j.anifeedsci.2011.03.014
Alemzadeh B, Taheri B, Kardooni A & Hosseini SM. 2015. Determining the digestibility of Mesquite fruit (Prosopis Juliflora) by in vivo method and degradability of seed and mesquite fruit by in situ method. Applied Animal Science Research Journal, 15: 65- 72.
Amerah AM, Ravindran V, Lentle RG & Thomas DG. 2007. Influence of feed particle size and feed form on the performance, energy utilisation, digestive tract development, and digesta parameters of broiler starters. Poultry Science, 86: 2615-2623. DOI: 10.3382/ps.2007-00212
Aminzadeh M, Beheshti M, Foroughi AR & Farzadmehr j. 2012. Determination of chemical composition and biodegradability components of Kahorak plant using nylon bag method. Master thesis. Birjand University.
AOAC. 2005. Association Official Methods of Analysis. 18th ed. AOAC International, Gaithersburg, MD.
Askari F. 2005. The nutritive value of twigs and fruits (Pods with seeds) Prosopis cineraria and Acacia tortilis. Journal of Animal Science, 68: 48-55.
Aviagen W. 2014. Ross 308: broiler nutrition specification. Aviagen Huntsville, Alabama, USA.
Azarbaijani A. 1995. Different methods of barley processing in feeding broiler chickens and laying hens. Master thesis. Isfahan University.
Bennett CD, Classen HL & Riddell C. 2002. Feeding broiler chickens wheat and barley diets containing whole, ground and pelleted grain. Poultry Science, 81: 995-1003. DOI: 10.1093/ps/81.7.995
Chaudhary RS, Vaishnave JK & Nehra R. 2005. Effect of replacing maize by mesquite pods (Prosopis juliflora) on the performance of broilers. Indian Journal of Poultry Science, 40(2): 124-127.
Chewning CG, Stark CR & Brake J. 2012. Effects of particle size and feed form on broiler performance. The Journal of Applied Poultry Research, 21: 830-837. DOI: 10.3382/japr.2012-00553
Choi JH, So BS, Ryu KS & Kang SL. 1986. Effects of pelleted or crumbled diets on the performance and the development of the digestive organs of broilers. Poultry Science, 65: 594-597. DOI: 10.3382/ps.0650594
Dashtban H, Afzali N, Hosseini Vashan SJ & Sarir H. 2016. The effect of Prosopis farcta on the performance, some blood parameters, immune and antioxidant system of broiler chickens under heat stress conditions. Journal of Animal Science, 114: 231-246. DOI: 10.22092/ASJ.2017.111325
De Oliveira Moraes GS, De Souza EJ & Véras AS. 2016. Total replacement of corn by mesquite pod meal considering nutritional value, performance, feeding behavior, nitrogen balance, and microbial protein synthesis of Holstein-Zebu crossbred dairy steers. Tropical Animal Health and Production, 48: 1415–1420. DOI: 10.1007/s11250-016-1108-4
Ebrahimi R, BojarPour M & Mokhtrazadeh S. 2010. Effects of feed particle size on the performance and carcass characteristics of broilers. Journal of Animal and Veterinary Advance, 9: 1482-1484. DOI: 10.1016/j.aninu.2015.06.001
Hetland H, Svihus B & Olaisen V. 2002. Effect of feeding whole cereals on performance, starch digestibility and duodenal particle size distribution in broiler chickens. British Poultry Science, 43(3): 416-423. DOI: 10.1080/ 00071660120103693
Hooshmandi AM, Bojarpour M, Yaghobfar A, Salari S & Rokni h. 2017. Influence of feed form, barley varieties and enzyme supplementation on performance, carcass characteristics and immune response of broilers. Animal Science Journal, 114: 257-272.
Hooshmandi AM, Yaghobfar A, Bojarpour M & Salari S. 2018. The effect of processing barley cultivars on intestinal morphology, enzyme activity and volatile fatty acids of the small intestine and serum lipid levels of broiler chickens. Iranian Journal of Veterinary Research, 73: 403-418. DOI: 10.22059/JVR.2018. 142263. 2429
Hussar N & Robblee AR. 1962. Effects of pelleting on the utilization of feed by the growing chicken. Poultry Science, 41: 1489-1493. DOI: 10.3382/ps.0411489
Kalantar M & Yaghobfar A. 2016. Animal model show physiological characteristics can alter by feeding of different cereal type and exogenous multi-enzyme. International Journal of Pharmacology, Phytochemistry and Ethnomedicine, 2: 13-19. DOI: 10.18052/ www.scipress.com/IJPPE.2.13
Kamran Z, Sarwar M, Nisa M, Nadeem MA, Ahmad S, Mushtaq T, Ahmad T & Shahzad MA. 2008. Effect of lowering dietary protein with constant energy to protein ratio on growth, body composition and nutrient utilization of broiler chicks. Asian-Australasian Journal of Animal Science, 21: 1629-1634. DOI: 10.5713/ajas. 2008.70586
Latshaw JD & Moritz JS. 2009. The partitioning of metabolisable energy by broiler chickens. Poultry Science, 88: 98-105. DOI: 10.3382/ps.2008-00161
Leenstra FR & Cahaner A. 1991. Genotype by environment interactions using fast-growing lean or fat broiler chickens, originating from the Netherland and Israel, raised at normal or low temperature. Poultry Science, 70: 2028-2039. DOI: 10.3382/ps.0702028
Mabray CJ & Waldroup W. 1981. The influence of dietary energy and amino acid level on abdominal fat pad development of broiler chickens. Poultry Science, 60: 151-159. DOI: 10.3382/ps.0600151
Makkar HPS. 2003. Quantification of tannins in tree and shrub folisge. In: Makkar, HPS.(Ed), A Laboratory Manual. Kluwer Academic Publishers, 102.
Mc-Cann MEE, Newell E, Perston C & Forbes K. 2006. The use of mannan-oligosaccharides and /or tannin in broiler diets. Journal of Poultry Science, 5(9): 873-879. DOI: 10.3923/ijps.2006.873.879
Moritz JS, Cramer KR, Wilson KJ & Beyer RS. 2003. Feed manufacture and feeding of rations with graded levels of added moisture formulated to different energy densities. Journal of Applied Poultry Research, 12: 371-381. DOI: 10.1093/ japr/12.3.371
Nir I, Twina Y, Grossman E & Nitsan Z. 1994. Quantitative effects of pelleting on performance, gastrointestinal tract and behavior of meat-type chickens. British Poultry Science, 35: 589-602. DOI: 10.1080/00071669408417724
Norouzi S, Yaghobfar A, Shekarpour M & Safamehr AR. 2013. Determination of nutritional value of mesquite pod and its different levels on performance of laying hens. Animal Science Journal, 4: 62-77.
Ozdal T, Capanoglu E & Altay F. 2013. A review on protein- phenolic interactions and associated changes. Food Research International, 51: 954-970. DOI: 10.1016/j.foodres.2013.02.009
Parsons AS, Buchanan NP, Blemings KP, Wilson ME & Moritz JS. 2006. Effect of corn particle size and pellet texture on broiler performance in the growing phase. Journal of Applied Poultry Research, 15: 245-255. DOI: 10.1093/japr/ 15.2.245
Rezaian M, Yaghobfar A & Barin J. 2007. Effects of pellet and mesh diets on the activity of the microflora, and morphology of the small intestine of broiler chicks. 16th European Symposium on Poultry Nutrition. Strasbourg, France.
Ruiz-Nieto JE, Herna´ndez-Ruiz J, Herna´ndez-Marı´n J, Mendoza-Carrillo J, Abraham-Jua´rez M, Isiordia-Lachica PM & Mireles-Arriaga AI. 2020. Mesquite (Prosopis spp.) tree as a feed resource for animal growth. Agroforestry Systems. DOI: 10.1007/s10457-020-00481-x
SAS Institute. 2004. SAS User’s Guide. Statistical Analytical System. Carry, NC.
Sharma R & Dakshini, KMM. 1998. Integration of plant and soil characteristics and the ecological success of two Prosopis species. Plant Ecology, 139: 63-69. DOI: 10.2307/20050695
Silva JHV, Silva EL, Filho JJ, Toledo RS, Albino LFT, Riberto MLG & Couto HP. 2002. Energy value and effect of integral mesquite pods meal inclusion in commercial laying hen diets. Revista Brasileira de Zootecnia, 31: 2255-2264. DOI: 10.1590/S1516-35982002000900014
Silva S. 1986. Prosopis juliflora (SW) DC in Brazil. The current state of knowledge on Prosopis juliflora. II International Conference on Prosopis recife, Brazil, pp: 29-51.
Toky OP, Arya S & Bisht RP. 1992. Ecological perspective of Prosopis cineraria (L). Duce in Arid and SEMi-Arid India. pp: 301-309.
Yaghobfar A & Boldaji F. 2002. Influence of level of feed input and procedure on metabolisable energy and endogenous energy loss (EEL) with adult cockerels. British Poultry Science, 43: 696-704. DOI: 10.1080/0007166021000025091
Yaghobfar A, Rezaian M, Ashrafi-Helan M, Barin J, Fazaeli H & Sharifi SD. 2006. The effect of hull-less barley dietary on the activity of gut microflora and morphology small intestinal of layer hens. Pakistan Journal of Biological Sciences, 9: 659-666. DOI: 10.3923/pjbs. 2006.659.666