Ileal Digestibility of Phosphorus in Plant Origin Feedstuffs Fed for Broiler Chickens: The Effect of Microbial Phytase

Document Type : Original Paper

Authors

1 Non-Ruminant Research Directorate, Tigray Agricultural Research Institute, Mekelle, Ethiopia.

2 Key Laboratory of Feed Biotechnology of Ministry of Agriculture, Feed Research Institute, Chinese Academy of Agricultural Sciences,12 Zhongguancun South Street, Haidian District, Beijing, China.

Abstract

The study was conducted to determine the apparent and standardized ileal digestibility (AID and SID) of phosphorus (P) in plant origin feedstuffs of corn distiller’s dried grains with solubles (corn DDGS), wheat bran (WB), wheat grain (WG), wheat middling (WM),soybean meal (SBM), canola meal (CNM), cottonseed meal (CSM), and peanut meal (PM), fed to total 612 male arbor acres broilers with or without microbial phytase. A 23 day-old birds with initial body weight (BW) of  939±8.89g were randomly allocated to a factorial 2 × 8 arrangement with a randomized complete block design of sixteen dietary treatments and one P-free diet, with six replicates (cage) and six birds/cage. A purified phosphorus-free (P-free) diet was also prepared to determined endogenous P loss (EPL). The ileal digesta were collected from euthanized birds and analyzed. The study results indicated that the SID of P in WB (38.46%), PM (37.98%), WG (36.90%), and WM (35.69%) was significantly lower (P < 0.001) than SBM (52.01%), corn DDGS (49.41%), CNM (47.02%), and CSM (46.09%) based diets. The Addition of microbial phytase to SBM and WM improved the SID of P by more than 40%, while the others improved by about 34.80%, which is from 31.55% in CNM to 37.77% in CSM. The least improvement was recorded in corn DDGS (5.12%). The feedstuffs ileal digestible P equivalent value of 1000 FTU/kg phytase was ranged from 0.2g to 1.6g. The ileal EPL of the current study was determined to be 46.28 mg/kg DMI. In conclusion, the application of microbial phytase and formulation of plant origin broiler diets based on SID of P can reasonably help to utilize phosphorus resources for reducing feed costs and minimize environmental pollution.

Keywords

References

Adedokun SA, Sands JS & Adeola O. 2004. Determining the equivalent phosphorus released by an Escherichia coli -derived phytase in broiler chicks. Journal of Animal Science, 84: 437–444. DOI: 10.4141/a03-117
Adeola O & Walk CL. 2013. Linking ileal digestible phosphorus and bone mineralization in broiler chickens fed diets supplemented with phytase and highly soluble calcium. Poultry Science, 92: 2109–2117. DOI: 10.3382/ps.2013-03068
Akinmusire AS & Adeola O. 2009. True digestibility of phosphorus in canola and soybean meals for growing pigs : Influence of microbial phytase. Journal of Animal Science, 87: 977–983. DOI: 10.2527/jas.2007-0778
Almaguer BL, Sulabo RC, Liu Y & Stein HH. 2014. Standardized total tract digestibility of phosphorus in copra meal, palm kernel expellers, palm kernel meal, and soybean meal fed to growing pigs. Journal of Animal Science, 92(6): 2473–2480. DOI: 10.2527/jas.2013-6654
Almeida FN& Stein HH. 2010. Performance and phosphorus balance of pigs fed diets formulated on the basis of values for standardized total tract digestibility of phosphorus. American Society of Animal Science, 88: 2968–2977. DOI: 10.2527/jas.2009-2285
AOAC (Association of Official analytical Chemists) International. 2007. Official Methods of Analysis of AOAC international. 18th ed. Hortwitz W, Latimer Jr., GW, editors. Gaithersburg, MD, USA.
Cromwell GL, Herkelman KL & Stahly TS. 1993. Physical, chemical, and nutritional characteristics of distillers dried grains with solubles for chicks and pigs. Journal of Animal Science, 71: 679–686. DOI: 10.2527/1993.713679x
Dayyani N, Beyki M, Abadi B, Amir A & Farhani A. 2013. Phytate and phytase in poultry nutrition. International Journal of Advanced Biological and Biomedical Research, 1: 1403–1408.
Dilger RN & Adeola O. 2006a. Estimation of true phosphorus digestibility and endogenous phosphorus loss in growing chicks fed conventional and low-phytate soybean meals. Poultry Science, 85: 661–668. DOI: 10.1093/ps/85.4.661
Dilger RN & Adeola O. 2006b. Estimation of true phosphorus digestibility and endogenous phosphorus loss in growing pigs fed conventional and low-phytate soybean meals. Journal of Animal Science, 84: 627–634. DOI: 10.2527/2006.843627x
Eeckhout W & De Paepe M. 1994. Total phosphorus, phytate-phosphorus and phytase activity in plant feedstuffs. Animal Feed Science and Technology, 47: 19–29. DOI: 10.1016/0377-8401(94)90156-2
Englen AJ, Heeft van der FC, Randsdrop PH, Somers WA, Schaefer J & vat van der BJ. 2001. Determination of phytase activity in feed by a colorimetric enzymatic method: collaborative interlaboratory study. Journal of AOAC International, 84: 629–633. DOI: 10.1093/jaoac/84.3.629
Fan MZ, Archbold T, Sauer WC, Lackeyram D, Rideout T, Gao Y, de Lange CF& Hacker RR. 2001. Novel methodology allows simultaneous measurement of true phosphorus digestibility and the gastrointestinal endogenous phosphorus outputs in studies with pigs. The Journal of Nutrition, 131: 2388–2396. DOI: 10.1093/jn/131.9.2388
Iyayi EA & Adeola O. 2013. True phosphorus digestibility of black-eyed pea and peanut flour without or with phytase supplementation in broiler chickens. Poultry Science, 92: 1595–1603. DOI: 10.3382/ps.2012-02898
Keulen JVan & Young BA. 1977. Evaluation of acid-insoluble ash as a natural marker in ruminant digestibility studies. Journal of Animal Science, 44: 282–287. DOI: 10.2527/jas1977.442282x
Leytem AB, Willing BP & Thacker PA. 2008. Phytate utilization and phosphorus excretion by broiler chickens fed diets containing cereal grains varying in phytate and phytase content. Animal Feed Science and Technology, 146: 160–168. DOI: 10.1016/j.anifeedsci.2007.11.006
Liu JB, Chen DW & Adeola O. 2013. Phosphorus digestibility response of broiler chickens to dietary calcium-to-phosphorus ratios. Poultry Science, 92: 1572–1578. DOI: 10.3382/ps.2012-02758
Liu S, Li S, Lu L, Xie J, Zhang L, Jiang Y & Luo X. 2012. Development of a procedure to determine standardized mineral availabilities in soybean meal for broiler chicks. Biological Trace Element Research, 148: 32–37. DOI: 10.1007/s12011-012-9332-x
Mutucumarana RK. 2014. Measurment of true ileal phosphorus digestibility in feed ingredients for poultry. PhD dissertation. Massey Universtity, Palmerston North, New Zealand. 208 Pages.
Mutucumarana RK, Ravindran V, Ravindran G & Cowieson AJ. 2014. Measurement of true ileal digestibility of phosphorus in some feed ingredients for broiler chickens. American Society of Animal Science, 92: 5520–5529. DOI: 10.1016/j.anifeedsci.2018.01.010
Nernberg LWJ. 1998. Improved phosphorus availability in poultry fed wheat/canola meal-based diets supplemented with phytase enzyme. Master’s thesis. The University of Manitoba, Canada. 117 pages.
NRC (National Research Council). 1994. Nutrient requirements of poultry. Ninth Revised Edition, National Academy Press, Washington, D.C.USA. 176 Pages.
Phillippy BQ. 1999. Susceptibility of Wheat and Aspergillus niger Phytases to Inactivation by Gastrointestinal Enzymes. Journal of Agricultural and Food Chemistry, 47: 1385–1388. DOI: 10.1021/jf981107x
Ravindran V, Ravindran G & Sivalogan S. 1994. Total and phytate phosphorus contents of various foods and feedstuffs of plant origin. Food Chemistry, 50: 133–136. DOI: 10.1016/0308-8146(94)90109-0
Rodehutscord M, Dieckmann A, Witzig M& Shastak Y. 2012. A note on sampling digesta from the ileum of broilers in phosphorus digestibility studies. Poultry Science, 91, 965–971. DOI: 10.3382/ps.2011-01943
Rojas OJ & Stein HH. 2012. Digestibility of phosphorus by growing pigs of fermented and conventional soybean meal without and with microbial phytase. Journal of Animal Science, 90: 1506–1512. DOI: 10.2527/jas.2011-4103
Rutherfurd SM, Chung TK, Morel PCH & Moughan PJ. 2004. Effect of microbial phytase on ileal digestibility of phytate phosphorus , total phosphorus , and amino acids in a low-phosphorus diet for broilers. Poultry Science, 83: 61–68. DOI: 10.1093/ps/83.1.61
Rutherfurd SM, Chung TK & Moughan PJ. 2002. The effect of microbial phytase on ileal phosphorus and amino acid digestibility in the broiler chicken. British Poultry Science, 44: 598–606. DOI: 10.1080/0007166022000004516
Salim HM, Kruk ZA & Lee BD. 2010. Nutritive value of corn distillers dried grains with solubles as an ingredient of poultry diets: A review. World’s Poultry Science Journal, 66: 411–432. DOI: 10.1017/S0043933910000504
Selle PH& Ravindran V. 2007. Microbial phytase in poultry nutrition. Animal Feed Science and Technology, 135: 1–41. DOI: 10.1016/j.anifeedsci.2006.06.010
Selle PH, Walker AR & Bryden WL. 2003. Total and phytate-phosphorus contents and phytase activity of Australian-sourced feed ingredients for pigs and poultry. Australian Journal of Experimental Agriculture, 43: 475–479. DOI: 10.1071/EA02155
Shastak Y & Rodehutscord M. 2013. Determination and estimation of phosphorus availability in growing poultry and their historical development. World’s Poultry Science Journal, 69: 569–586. DOI: 10.1017/S0043933913000585
She Y, Su Y, Liu L, Huang C, Li J, Li P, Li D & Piao X. 2015. Effects of microbial phytase on coefficient of standardized total tract digestibility of phosphorus in growing pigs fed corn and corn co-products, wheat and wheat co-products and oilseed meals. Animal Feed Science and Technology, 208: 132–144. DOI: 10.1016/j.anifeedsci.2015.07.011
Tran GD & Sauvant D. 2004. Chemical data and nutritional value. In Sauvant D, Perez JM, &Tran G. (Eds). Tables of composition and nutritional value of feed materials (2nd ed.). Wageningen Academic Publishers, Wageningen, The Netherlands.pp.17-24. DOI: 10.3920/978-90-8686-668-7
Viveros A, Centeno C, Brenes A, Canales R & Lozano A. 2000. Phytase and acid phosphatase activities in plant feedstuffs. Journal of Agricultural and Food Chemistry, 48: 4009–4013. DOI: 10.1021/jf991126m
Wu YB, Ravindran V & Hendriks WH. 2004. Influence of exogenous enzyme supplementation on energy utilisation and nutrient digestibility of cereals for broilers. Journal of the Science of Food and Agriculture, 84: 1817–1822. DOI: 10.1002/jsfa.1892
Poultry Science Journal
Volume 8, Issue 2
November 2020
Pages 201-210

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