Supplementation of Sodium Selenite and Methionine on Concentration of Selenium in Egg and Serum, Antioxidant Enzymes Activity and Immune Response of Iranian Native Broiler Breeders

Document Type : Original Paper


Department of Animal Science, Sari Agricultural Sciences and Natural Resources University, Sari, Iran


Effects of sodium selenite (SS) in offspring of broiler breeders has been reported, but the comparison between SS and different level of methionine on offspring has received limited, so this study was conducted to investigate sodium selenite and methionine effects on concentration of selenium in egg and serum of Iranian native broiler breeders. An experiment was conducted in 3 × 3 factorial experiment with three levels of sodium selenite (0.0, 0.3, and 0.5 mg/kg of diet) and three levels of methionine (0.23, 0.31 and 0.33 % of diet) to investigate reproductive performance and immune status of broiler breeders (64-74 wks). The higher methionine levels increased the egg weight until the dose of 0.31% of diet (P < 0.05). In this experiment, 0.5 mg/kg SS with 0.31 % methionine in the diet was found to increase egg weight (P < 0.05). The highest salable chicks were obtained by supplementing 0.3 mg/kg SS (P= 0.07). Increasing the level of methionine from 0.23 to 0.33% without SS and also, supplementation SS at 0.3 mg/kg with 0.33 % methionine exhibited higher serum selenium, although, no effect of methionine levels was observed on concentration of selenium in serum and transfer rate. Concentration of selenium in eggs and serum were significantly affected by interaction of SS and methionine levels in diet. An increment in glutathione peroxidase (GSH-Px) level tended to increase with SS (0.5 mg/kg) and methionine (0.33%) interaction (P=0.06). The results showed that increasing the level of methionine increases the immune response against influenza (AI) and Newcastle disease (ND) (P = 0.0001). These results suggest that higher level of methionine at 0.31% could improve egg weight and Influenza and ND titers, SS at dose of 0.5 could increase concentration of selenium in serum, whereas increasing of SS levels significantly raised embryonic mortality.


Abdalla A, Yakout HM & Khalifah MM. 2005. Determination of lysine and methionine requirements of gimmizah strain during egg production stage. Proc. 3rd Inter. Poultry Conference Egypt.
Abd-EL-Samee MO, EL-Sheikh TM & Ali AM. 2007. An attempt to alleviate heat stress and improve laying hens performance and egg quality during summer season by using some amino acids or vitamins. Egypt. Poultry Science, 27: 875- 891.
Arthur JR. The glutathione peroxidases. 2000. Cellular and Molecular Life Sciences, 57: 1825–1835.
Boruta A, Oewierczewska E & Roszkowski T. 2007. Effect of organic and inorganic forms of selenium on the morphological composition of eggs and selenium content in egg mass.  Medycyna Weterynaryjna (In Polish), 63: 238-241.
Brigelius-Flohe R.1999.  Tissue-specific functions of individual glutathione peroxidases. Free Radical Biology and Medicine, 27: 951–965. DOI: 10.1016/S0891-5849(99)00173-2
Broome CS, McArdle F, Kyle JA, Andrews F, Lowe NM & Hart CA. 2004. An increase in selenium intake improves immune function and poliovirus handing in adults with marginal selenium status. The American Journal of Clinical Nutrition, 80: 154-62. DOI: 10.1093/ajcn/80.1.154
Bunchasak C & Silapasorn T. 2005. Effects of adding methionine in low-protein diet on production performance, reproductive organs and chemical liver composition of laying hens under tropical conditions. International Journal of Poultry Science, 4: 301-308. DOI:10.3923/ ijps.2005. 301.308
Carter TC. 1975.  The hen’s egg: Estimation of shell superficial area and egg volume, using measurements of fresh egg weight and shell length and breadth alone or in combination. British Poultry Science, 16: 541-543. DOI: 10.1080/00071667508416224
Cousins FB & Cairney M. 1961. Some aspects of selenium metabolism in sheep. Australian Journal of Agricultural Research, 12: 927–943. DOI: 10.4141/cjas68-045
Delezie E, Rovers M, Van der Aa A, Ruttens A, Wittocx S & Segers L. 2014. Comparing responses to different selenium sources and dosages in laying hens. Poultry Science, 93: 3083–3090. DOI: 10.3382/ps.2014-04301
Dziaczkowska L. 1980. The pattern for the assessment of turkey chicks for fattening. In polish.; Centre for Poultry Research and Development, Center for Water Fowl. Poznan.
Fairweather-Tait S & Hurrell RF. 1996. Bioavailability of minerals and trace elements. Nutation Research Review, 9: 295–324. DOI: 10.1079/NRR19960016
Hoffmann PR & Marla JB. 2008.  The influence of selenium on immune responses.  Molecular Nutrition and Food Research, 52: 1273–1280. DOI: 10.1002/mnfr.200700330
Hatfield DL & Gladyshev VN. 2002. How selenium has altered our understanding of the genetic code. Molecular and Cell Biology, 22: 3565–3576. DOI: 10.1002/mnfr.200700330
Hempe JM & Laukxen RC. 1988. Savage JE. Rapid determination of egg weight and specific gravity using a computerized data collection system. Poultry Science, 67: 902-907.  DOI: 10.3382/ ps.0670902
Jackson ME, Hellwig HM & Waldroup WP. 1987. Shell quality: Potential for improvement by dietary means and relationship with egg size. Poultry Science, 66: 1702–1713. DOI: 10.3382/ ps.0661702
Jing CL, Dong XF, Wang ZM, Liu S & Tong JM. 2015. Comparative study of DL-[Se] Met vs SS and seleno-yeast on antioxidant activity and selenium status in laying hens. Poultry Science, 94:965–975. DOI: 10.3382/ps/pev045
Kazemi‐Fard M, Kermanshahi H, Rezaei M & Golian A. 2013. Effect of different levels of fennel extract and vitamin D3 on performance, hatchability and immunity in post molted broiler breeders. Iranian Journal of Animal Science, 3: 735-745.
Kenyon S & Spring P. 2003. Egg selenium concentrations in breeder’s hens fed Na – selenite or Sel Plex® supplemented diets. XthEuropean Symp. On the quality of eggs and egg poducts, September 23-26, Saint – Brieuc – Ploufragan, Cotes d’ Armor France, pp. 182.
Knight SAB & Sunde RA. 1988. Effect of selenium repletion on glutathione peroxidase protein in rat liver. Journal of Nutrition, 118: 853-58
Kurz B, Jost B & Sch¨unke M. 2002. Dietary vitamins and selenium diminish the development of mechanically induced osteoarthritis and increase the expression of antioxidative enzymes in the knee joint of STR/1N mice. Osteoarthritis and cartilage, 10: 119–126. DOI: 10.1053/joca. 2001.0489
Latshaw JD, Morishita TY, Sarver CF & Thilsted J. 2004. Selenium toxicity in breeding Ring-Necked pheasants (Phasianus colchicus). Avian Diseases, 48: 935-939. DOI: 10.1637/7177-030904R
Leeson S, Namkung H, Caston L, Durosoy S & Schlegel P. 2008. Comparison of selenium levels and sources and dietary fat quality in diets for broiler breeders and layer hens. Poultry Science, 87: 2605–2612. DOI: 10.3382/ps.2008-00174
Lescure A, Gautheret D & Krol A. 2002. Novel selenoproteins identified from genomic sequence data. In: Methods in Enzymology 347 (Sies, H. and Packer, L., eds.), pp.; 57–70. Academic Press, San Diego, CA.
McConnell KP & Cho GJ. 1965.  Transmucosal movement of selenium. American Journal of Physiology, 208: 1191–1195. DOI: 10.1152/ ajplegacy.1965.208.6.1191
McKenzie RC, Arthur JR & Beckett GJ. 2002. Selenium and the regulation of cell signaling, growth, and survival: molecular and mechanistic aspects. Antioxidants and Redox. Signaling, 4: 339–351. DOI: 10.1089/152308602753666398
Nassiri-Moghaddam H, Kazemi-Fard M, Agah MJ, Hosseini SJ & Mirakzehi MT. 2012.  Effect of different levels of methionine, protein and tallow on the productive performance and egg quality of laying hens in the late-phase production. Brazilian Journal of Poultry Science, 14: 149-158. DOI: 10.1590/S1516-635X2012000200010 
Naulia AH & Singh MA. 2002. Effect of dietary protein and supplemental fat on performance of laying hens. International Journal of Poultry Science, 4: 1986-1989. DOI: 10.3923/ijps. 2005.986.989
Ort JF & Latshaw JD.1978.  The toxic level of SS in the diet of laying hens. Journal of Nutrition, 108: 1114–1120.http:// doi:10.1093/jn/108.7.1114
Ousterhout LE. 1980. Effects of calcium and phosphorus levels on egg weight and egg shell quality in laying hens. Poultry Science, 59: 1480-1484. DOI: 10.3382/ps.0591480
Pappas AC, Acmovic T, Sparks NHC, Surai PF & McDevitt RM. 2006. Effects of supplementing broiler breeders diets with organoselenium compounds and polyunsaturated fatty acids on hatchability. Poultry Science, 85: 1584–1593. DOI: 10.3382/ps.0591480
Payne RL & Southern LL. 2005. Changes in GSH-Px and tissue selenium concentrations of broilers after consuming a diet adequate in selenium. Poultry Science, 84: 1268–1276. DOI: 10.1093/ps/84.8.1268
Payne RL, Lavergne TK & Southern LL. 2005. Effect of inorganic versus organic selenium on hen production and egg selenium concentration. Poultry Science, 84: 232–237. DOI: 10.1093/ ps/84.2.232
Petersen CF, Sauter EA, Steele EE & Parkinson JF. 1983. Use of methionine intake restriction to improve egg shell quality by control of egg weight. Poultry Science, 62: 2044–2047. DOI: 10.3382/ps.0622044
Reffett JK, Spears JW & Brown TT. 1988. Effect of dietary selenium on the primary and secondary immune response in calves challenged with infectious bovine rhinotracheitis virus. Journal of Nutrition, 118: 229–235. DOI: 10.1093/ jn/118.2.229
Reis RN, Vieira SL, Nascimento PC, Pena JE, Barros R & Torres CA. 2009.  Selenium contents of eggs from broiler breeders supplemented with SS or zinc-l-selenium methionine. Journal of Applied Poultry Research, 18: 151–157. DOI: 10.3382/ japr.2008-00069
Robinson MF & Thomson CD. 1983. The role of selenium in the diet. Nutrition Abstracts and Reviews, 53. 3-26.
Safaa HM, Serrano MP, Valencia DG, Arbe X, Jime ́nez-Moreno E, La ́zaro R & Mateos GG. 2008. Effects of the levels of methionine, linoleic acid, and added fat in the diet on productive performance and egg quality of brown laying hens in the late phase of production. Poultry Science, 87: 1595–1602. DOI: 10.3382/ps.2008-00005
SAS Institute. 2003. SAS/STAT 9.1.3 User’s Guide. SAS Inst. Inc., Cary, NC.
Susanne B, Erik HL, Jens JS, Knut F, Kim O, Lars CS & Sven M. 2008. Absorption, excretion, and retention of selenium from a high selenium yeast in men with a high intake of selenium. Food Nutrition Research, 52: 10.3402.
Skrivan M, Bubancova I, Marounek M & Dlouha G. 2010. Selenium and alpha-tocopherol content in eggs produced by hens that were fed diets supplemented with selenome­thionine, SS and vitamin E. Czech. Journal of Animal Science, 55: 388–397. DOI: 10.17221/92/2010-CJAS
Spallholz JE. 1990. Selenium and glutathione peroxidase: essential nutrient and antioxidant component of the immune system. Advances in Experimental Medicine and Biology, 262: 145–158.
Szeleszczuk P, Karpinska E, Bielecki W, Kosowska G &Borzemska W. 2004.  Evaluation of lithium and selenium toxicity for chicken embryos and one-day-old chicks. Medycyna Weterynaryjna, 60: 492-495.
Tahmasbi AM, Mirakzehi MT, Hosseini SJ, Agah MJ & Kazemi-Fard M. 2012. The effects of phytase and root hydroalcoholic extract of Withania somnifera on productive performance and bone mineralization of laying hens in the late phase of production. British Poultry Science, 53: 204-214. DOI: 10.1080/00071668.2012.662628
Utterback PL, Parsons CM, Yoon I & Butler J. 2005.  Effect of supplementing selenium east in diets of laying hens on egg selenium content. Poultry Science, 84: 900–1901. DOI: 10.1093/ps/ 84.12.1900
Vendeland SC, Butler JA & Whanger PD. 1992. Intestinal absorption of selenite, selenate, and selenomethionine in the rat. Journal of Nutrition Biochemistry, 3: 359-365. DOI: 10.1016/0955-2863(92)90028-H
Vendeland SC, Deagen JT & Whanger PD. 1996. Uptake of selenotrisulfides of glutathione and cysteine by brush border membranes from rat intestines. Journal of Inorganic Biochemistry, 47: 131-140.
Waschulewski H & Sunde RA. 1988. Effect of dietary methionine on utilization of tissue selenium from dietary selenomethionine for glutathione peroxidase in the rat. Journal of Nutrition, 118: 367-374. DOI:10.1093/jn/ 118.3.367
Waschulewski H & Sunde RA. 1988. Effect of dietary methionine on tissue selenium and glutathione peroxidase (EC activity in rats given selenomethionine. British Journal of Nutrition, 60: 57-68. DOI: 10.1093/jn/118.3.367
Willemsen H, Swennen Q & Everaert N. 2011. Effects of dietary supplementation of methionine and its hydroxy analog DL-2-hydroxy-4-methylthiobutanoic acid on growth performance, plasma hormone levels, and the redox status of broiler chickens exposed to high temperatures. Poultry Science, 90: 2311-20. DOI: 10.3382/ ps.2011-01353
Wolffram S, Berger B, Grenacher B & Scharrer E. 1989. Transport of selenoamino acids and their sulfur analogues across the intestinal brush border membrane of pigs. Journal of Nutrition, 119: 706-712. DOI: 10.1093/jn/119.5.706
Xue X, Wang Y,  Liu W,  Ju T & Zhan X. 2017.  Effects of different methionine sources on production and reproduction performance, egg quality and serum biochemical indices of broiler breeders.  Asian-Australian Journal of Animal Science, 30: 828–833. DOI: 10.5713/ajas.16.0404
Yoon I, Werner T & Butler J. 2007. Effect of source and concentration of selenium on growth performance and selenium retention in broiler chickens. Poultry Science, 86: 727–730. DOI: 10.1093/ps/86.4.727