Effects of Long-term Induced Hyperthyroidism on Egg Quality Traits in Cobb 500 Broiler Breeder Hens

Document Type : Original Paper


1 Department of Animal and Poultry Physiology, Faculty of Animal Science, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran

2 Department of Animal Science, College of Agriculture, Shiraz University, Shiraz, Iran

3 Department of Animal Science, Sari Agricultural Sciences and Natural Resources University, Sari, Iran

4 Department of Animal Science, University College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran


Published data on 4-week-long administration of exogenous thyroxine in broiler breeder hens to decline the cold-induced ascites in their progeny suggest that the long-term maternal hyperthyroidism would affect egg quality characteristics traits in Cobb 500 broiler breeder hens. Seventy 47-w-old broiler breeder Cobb 500 hens (5 replicates and 7 hens each) were assigned in separate cages and allotted to two treatments, control and hyperthyroid. Thyroxine was orally administered to the hyperthyroid group (0.3 mg hen/day) for a period of 100 days consecutively. Simultaneously, distilled water was orally administered to the control group. The blood sampling was done every two weeks to analyze T3, T4, and estrogen assays, using commercially kits and the egg quality attributes were evaluated for weeks 0, 3, 6, 9, and 12. Thyroxine treatment resulted in an increase in plasma concentration of T4; however, the T3 level and estrogen were not affected. The results of this study showed that the long-term administration of thyroxine had adverse effect on the most of egg quality traits in broiler breeder hens; although the results may be distinct for treatments that using other birds, doses and duration of treatments, among the different egg quality traits of broiler breeder hens evaluated in this research. Therefore, further studies should be done to make a final conclusion to use of long-term maternal hyperthyroidism treatment to reduce the ascites incidence.


Akhlaghi A, Zamiri MJ, ZareShahneh A, JafariAhangari Y, NejatiJavaremi A, RahimiMianji G, Mollasalehi MR, Shojaie H, Akhlaghi AA, Deldar H &Atashi H. 2012. Maternal hyperthyroidism is associated with a decreased incidence of cold-induced ascites in broiler chickens. Poultry Science, 91: 1165-1172. DOI: 10.3382/ps.2011-02021
Akhlaghi A, Zamiri MJ, JafariAhangari Y, Atashi H, Ansari Pirsaraei Z, Deldar H, Eghbalian AN, Akhlaghi AA, Navidshad B, YussefiKelarikolaei K &Hashemi SR. 2013. Oral exposure of broiler breeder hens to extra thyroxine modulates early adaptive immune responses in progeny chicks. Poultry Science, 92: 1040-1049. DOI: 10.3382/ps.2012-02545
Benton Jr CE, Walsh TG & Brake J. 2001. Effects of presence of a blastoderm on albumen height and pH of broiler hatching eggs. Poultry Science, 80: 955-957. DOI: 10.1093/ps/80.7.955
Brake J, Walsh TJ, Benton Jr. CE, Petitte JN, Meijerhof R &Penalva G. 1997. Egg handling and storage. Poultry Science, 76: 144–151. DOI: 10.1093/ps/76.1.144
Buys N, Buyse J, Hassanzadeh-Ladmakhi M &Decuypere E. 1998. Intermittent lighting reduces the incidence of ascites in broilers: An interaction with protein content of feed on performance and the endocrine system. Poultry Science, 77: 54–61. DOI: 10.1093/ps/77.1.54
Christensen VL & Ort JF. 1990. Influence of diet-mediated maternal thyroid alterations on functional properties of turkey eggs. Poultry Science, 69: 1576-1581. DOI: 10.3382/ps.0691576
Davidson MF. 1986. Histological studies of changes in the magnum of the domestic hen associated with the production of watery white eggs. British Poultry Science, 27: 353–354. DOI: 10.1080/00071668608416889
Geng AL, Guo YM & Yang Y. 2004. Reduction of ascites mortality in broilers by coenzyme Q10. Poultry Science, 83: 1587–1593. DOI: 10.1093/ps/83.9.1587
Klandorf H, Blauwiekel R, Qin X&Russell RW. 1992. Characterization of a sustained-release estrogen implant on oviduct development and plasma Ca concentrations in broiler breeder chicks: modulation by feed restriction and thyroid state. General and Comparative Endocrinology,86: 469-482. DOI: 10.1016/0016-6480(92)90072-R
Kohler PO, Grimley PM&O’Malley BW. 1969. Estrogen-induced cytodifferentiation of the ovalbumin-secreting glands of the chick oviduct. The Journal of Cell Biology 40: 8-27. DOI: 10.1083/jcb.40.1.8
Lapão C, Gama LT &Soares MC. 1999. Effects of broiler breeder age and length of egg storage on albumen characteristics and hatchability. Poultry Science, 78: 640–645. DOI: 10.1093/ps/78.5.640
Luger D, Shinder D &YahavS. 2002. Hyper- or hypothyroidism: Its association with the development of ascites syndrome in fast-growing chickens. General and Comparative Endocrinology, 127: 293–299. DOI: 10.1016/S0016-6480(02)00050-3
Ocampo L, Cortez U, Sumano H & Avila E. 1998. Use of low doses of clenbuterol to reduce incidence of ascites syndrome in broilers. Poultry Science, 77: 1297–1299. DOI: 10.1093/ps/77.9.1297
Pavlidis HO, Balog JM, Stamps LK, Hughes Jr. JD, Huff WE & Anthony NB. 2007. Divergent selection for ascites incidence in chickens. Poultry Science, 86: 2517–2529. DOI: 10.3382/ps.2007-00134
Peebles ED, Miller EH, Boyle CR, Brake JD & Latour MA. 1994. Effects of dietary thiouracil on thyroid activity, egg production, and eggshell quality in commercial layers. Poultry Science, 73: 1829-1837. DOI: 10.3382/ps.0731829
Peebles ED, Miller EH, Brake JD & Schultz CD. 1992. Effects of ascorbic acid on plasma thyroxine concentrations and eggshell quality of Leghorn chickens treated with dietary thiouracil. Poultry Science, 71: 553-559. DOI: 10.3382/ps.0710553
Reijrink IAM. 2010. Storage of hatching eggs: Effects of storage and early incubation conditions on egg characteristics, embryonic development, hatchability, and chick quality. Ph.D. thesis. Wageningen University, the Netherlands.
Roberts JR. 2004. Factors affecting egg internal quality and egg shell quality in laying hens. The Journal of Poultry Science, 41: 161-177. DOI: 10.2141/jpsa.41.161
Saemi F, Shahneh AZ, Zhandi M, Akhlaghi A &Ansari Pirsaraei Z. 2018a.TGF-β4 and HSP70 responses in breeder hens treated with thyroxine. Animal Reproduction Science. In press. DOI: 10.1016/j.anireprosci.2018.09.004
Saemi F, Shahneh AZ, Zhandi M, Akhlaghi A, Khaksar Z &Dadpasand M. 2018b. Long-term effects of thyroxine-induced hyperthyroidism on the histological attributes of the oviduct in broiler breeder hens. Comparative Clinical Pathology, 27: 605-609. DOI: 10.1007/s00580-017-2634-9.
SAS. 2003. User’ s Guide: Statistics, Version 9.1 Edition. Inst., Inc., Cary, NC.
Sechman A. 2013. The role of thyroid hormones in regulation of chicken ovarian steroidogenesis. General and Comparative Endocrinology, 190: 68-75. DOI: 10.1016/j.ygcen.2013.04.012
Shlosberg A, Berman E, Bendheim U &Plavnik I. 1991. Controlled early feed restriction as a potential means of reducing the incidence of ascites in broilers. Avian Diseases, 35: 681–684. DOI: 10.2307/1591596
Solis de los Santos F, Farnell MB, Tellez G, Balog JM, Anthony NB, Torres-Rodriguez A, Higgins S, Hargis BM & Donoghue AM. 2005. Effect of prebiotic on gut development and ascites incidence of broilers reared in a hypoxic environment. Poultry Science, 84:1092–1100. DOI: 10.1093/ps/84.7.1092
Zheng QH, Jiang YB, Guo JL, Yin QQ& Cheng W. 2007. Study on mechanism of ascites syndrome of broilers.Research Journal of Animal Sciences, 1: 72-75. DOI: 10.3844/ajavsp.2007.62.65.