Comparing the Effects of Supplementary Antibiotic, Probiotic, and Prebiotic on Carcass Composition, Salmonella Counts and Serotypes in Droppings and Intestine of Broiler Chickens

Document Type : Original Paper

Authors

1 Department of Animal Nutrition, Federal University of Agriculture, P.M.B. 2240, Abeokuta, Nigeria

2 Department of Food Science and Technology, Federal University of Agriculture, P.M.B. 2240, Abeokuta, Nigeria

3 Department of Animal Production and Health, Federal University of Agriculture, P.M.B. 2240, Abeokuta, Nigeria

Abstract

The effects of antibiotic, probiotic, and prebiotic as dietary feed additives on carcass composition as well as Salmonella counts and its serotypes in droppings and intestine of broiler chickens on days 1, 28, and 56 of experiment were investigated. Dietary treatments were control diet (basal diet without additives), OXYT diet (basal diet with 600 ppm of the antibiotic oxytetracycline), GRO-UP diet (basal diet with 500 ppm probiotic), and MOS-500 or MOS-1000 diets (basal diet with 500 or 1000 ppm mannan oligosaccharide prebiotic, respectively). From an initial total of 190 day-old Arbor acres broiler chicks, two birds were randomly selected from each treatment and sampled for Salmonella. The remaining 180 birds were randomly allotted to the five dietary treatments with three replicates of 12 birds each. Feed and water were supplied ad libitum. The results indicated that breast, neck, drumsticks and liver yields significantly affected by the inclusion of feed additives (p < 0.05). Significant differences were observed in averageSalmonella counts in both the intestines and droppings of broiler chickens at different days of experiment (p < 0.05). Salmonella Gallinarumand Salmonella Typhi were serotypes identified in the droppings and intestine of broiler chickens. Our findings reveal that the inclusion of prebiotic oligomanno or probiotic GRO-UP as antibiotic substitutes in diets of broiler chickens can rapidly reduce some Salmonellaserotypes and aid to the control Salmonellaorganisms.

Keywords

References

Agunos A, Ibuki M & Mine Y. 2007. Effect of dietary βl-4 mannobiose in the prevention of Salmonella enteritidis infection in broilers. British Poultry Science, 48: 331-341. DOI: 10.1080/00071660701370442
Alam S. 2000. Handbook of poultry disease and treatment. 9th Ed. Anglo Egyptian Library Press, Egypt in Arabic. Pages, 28, 34 & 40.
Bailey JS, Blankenship LC & Cox NA. 1991. Effect of fructooligosaccharide on Salmonella colonization of the chicken intestine. Poultry Science, 70: 2433–2438. DOI : 10.3382/ps.0702433
Bunyan J, Jeferies L, Sayers JR, Gulliver AL & Colemon K. 1997. Antimicrobial substances and chick growth promotion: The growth-promoting activities of antimicrobial substances, including fifty-two used either in therapy or as dietary additives. British Poultry Science, 18: 283-294. DOI: 10.1080/00071667708416364
Clementino dos Santos E, Soares Teixeira A, Gilberto Bertechini R, Tadeu Fonseca de Freitas P, Borges Rodregues E, Souza Dias DM, Torres DM, Vierra Santos A & Giacometi RA. 2002. Effects of growth beneficial additives on broiler carcass yield. Federal Agrotech. School of Inconfidentes and Federal University of Lavras, Minas Gerais, Brazil. In: Proceedings of Brazilian Society of Animal Production (Sociedade Brasileria de Zootecnia). Page, 5.
Collins CH, Lyne PM, Grange JM & Falkinham JO. 2004. Identification methods. In: Collins CH, Lyne PM, Grange JM & Falkinham JO. (Eds). 8th Ed. Collins and Lyne's Microbiological Methods. Arnold. Hodder Headline Group. London. 512 Pages.
Courtin CM, Broekaert WF,  Swennen K, Lescroart O, Onagbesan O, Buyse J, Decuypere E, Van de Wiele T, Marzorati M, Verstraete W, Huyghebaert G & Delcour JA. 2008. Dietary inclusion of wheat bran arabinoxylooligosaccharides induces beneficial nutrirional effects on chickens. Cereal Chemistry, 85: 607-613. DOI: 10.1094/ CCHEM-85-5-0607
Davies RH & Wray C. 1993. Salmonellapollution in poultry units and associated enterprises; Pollution in livestock production system. Dew A, Axford R & Marai FM. (Eds). International, Wallingford, UK. 463 Pages.
Eltazi SMA. 2014. Response of broiler chicks to diets containing different mixture levels of garlic and ginger powder as natural feed additives.International Journal of Pharmaceutical Research & Allied Sciences, 3: 27-35.
Fairchild AS, Smith JL, Idris U, Lu J, Sanchez S, Purvis LB, Hofacre C & Lee MD.2005. Effects of orally administered tetracycline on the intestinal community structure of chickens and on tet determinant carriage by commensal bacteria and Campylobacter jejuni. Applied and Environmental Microbiology, 71: 5865–5872. DOI: 10.1128/AEM.71.10.5865-5872.2005
FASS (Federation of Animal Science Societies). 2010. Guide for the Care and Use of Agricultural Animals in Research and Teaching, 3rd Ed. Federation of Animal Science Societies, Champaign-Urbana, Illinois, IL.
Fernandez F, Hinton M & Van Gils B. 2000. Evaluation of the effect of mannan-oligosaccharides on the competitive exclusion of Salmonellaenteritidis colonization in broiler chicks. Avian Pathology, 29: 575-581. DOI: 10.1080/03079450020016823
Fuller R. 1989. Probiotics in man and animals. Journal of Applied Microbiology, 66: 365-378. DOI: 10.1111/j.1365-2672.1989.tb05105.x
Gibson GR & Roberfroid MB. 1995. Dietary modulation of human colonic microbiota: Introducing the concept of prebiotic. Journal of Nutrition, 125: 1401- 1412.
Google Earth. 2013. Google location map; Google earth imagery date; December 3rd, 2013.
Hendriksen RS. 2003. Identification of Salmonella. Global Salm-Surv. A global Salmonella surveillance and laboratory support project of the World Health Organization. Pages, 1-6.
Higgins SE, Higgins JP, Wolfenden AD, Henderson SN, Torres-Rodriguez A, Tellez G & Hargis B. 2008. Evaluation of a lactobacillus-based probiotic culture for the reduction of Salmonella enteritidis in neonatal broiler chicks. Poultry Science, 87: 27–31. DOI: 10.3382/ps.2007-00210
Huyghebaert G, Ducatelle R & Immerseel FV. 2011. An update on alternatives to antimicrobial growth promoters for broilers. The Veterinary Journal, 187(2):182–188. DOI: 10.1016/j.tvjl.2010.03.003
Jensen JF. 1984. Method of dissection of broiler carcasses and description of parts. World’s Poultry Science Association European Federation Working Group V, Copenhagen. p. 32.
Jin LZ, Ho YW, Abdullah N & Jalaludin S. 1997. Probiotics in poultry: Modes of action. World’sPoultry Science Journal, 53: 351–368. DOI: 10.1079/WPS19970028
Marković R, Šefer D, Krstić M & Petrujkić B. 2009. Effect of different growth promoters on broiler performance and gut morphology. Archivos de Medicina Veterinaria, 41: 163–169.
Mathis GF. 2000. Effect of MOS on growth and feed efficiency in commercial broiler chickens. Southern Poultry Research, Inc., Athens, Georgia USA.
Midilli M, Alp M, Kocabach N, Muglah ÖH, Turan N, Yilmaz H & Çakir S. 2008. Effects of dietary probiotic and prebiotic supplementation on growth performance and serum IgG concentration of broilers. South African Journal of Animal Science, 38: 21-27.
Mohammed MA, Hassan HMA & El-Barkouky EMA. 2008. Effect of mannan oligosaccharide on performance and carcass characteristics of broiler chicks. Journal of Agriculture and Social Sciences, 4: 7-13.
Mohammadi Gheisar M, Nyachoti CM, Hancock JD & Kim IH. 2016. Effects of lactulose on growth, carcass characteristics, faecal microbiota, and blood constituents in broilers. Veterinarni Medicina, 61:  90-96. DOI: 10.17221/8722-VETMED
Nataro JP, Bopp CA, Fields PI, Kaper JB & Strockbine NA. 2011. Escherichia, Shigella, and Salmonella. In: Versalovic J, Carroll K, Funke G, Jorgensen J, Landry ML & Warnock EW. (Eds). Manual of Clinical Microbiology. 10th Ed. Vol 1. Washington DC: ASM Press. 2630 Pages. DOI: 10.1128/9781555816728.ch35
Oyofo BA, DeLoach JR, Corrier DE, Norman JO, Ziprin RL & Molenhauer HH. 1989. Prevention of Salmonella typhimurium colonization of broilers with D-mannose. Poultry Science, 68: 1357-1360. DOI: 10.3382/ps.0681357
Park SH, Kim HJ, Cho WH, Kim JH, Oh MH, Kim SH, Lee BK, Ricke SC & Kim HY. 2009. Identification of Salmonella enterica subspecies I, Salmonella enterica serovars Typhimurium, Enteritidis and Typhi using multiplex PCR. FEMS Microbiology Letters, 301: 137-146. DOI: 10.1111/j.1574-6968.2009.01809.x
Pascual M, Hugas M, Badiola JI, Monfort JM & Garriga M. 1999. Lactobacillus salivarius CTC2197 prevents Salmonella enteritidis colonization in chickens. Applied and Environmental Microbiology, 65: 4981–4986.
Patterson JA & Burkholder KM. 2003. Application of prebiotics and probiotics in poultry production. Poultry Science, 82: 627–631. DOI: 10.1093/ps/82.4.627
Peterz M, Wiberg C & Norberg P. 1989. The effect of incubation temperature and magnesium chloride concentration on growth of Salmonellain home-made and in commercially available dehydrated Rappaport-Vassiliadis broths. Journal of Applied Microbiology, 66: 523-528. DOI: 10.1111/j.1365-2672.1989.tb02535.x
Public Health England (PHE). 2015. Bacteriology – Identification of Salmonella species. UK Standards for Microbiology Investigations. Standards Unit, Microbiology Services, Public Health England. Issue no 3. Pages, 1-23.
Rappaport F, Konforti N & Navon B. 1956. A new enrichment medium for certain Salmonellae. Journal of Clinical Pathology, 9: 261-266.
SAS (Statistical Analysis System). 2003. SAS/STAT(R) User’s Guide. SAS Institute Inc., Cary, North Carolina, USA.
Simon O, Jadamus A & Vahjen W. 2001. Probiotic feed additives – effectiveness and expected modes of action. Journal of Animal and Feed Sciences, 10: 51–67.
Spring P, Wenk C, Dawson KA & Newman KE. 2000. The effects of dietary mannaoligosaccharides on cecal parameters and the concentrations of enteric bacteria in the ceca of Salmonella-challenged broiler chicks. Poultry Science, 79: 205–211. DOI: 10.1093/ps/79.2.205
Stern NJ, Cox NA, Bailey JS, Berrang ME & Musgrove MT. 2001. Comparison of mucosal competitive exclusion and competitive exclusion treatment to reduce Salmonella and Campylobacter spp colonization in broiler chickens. Poultry Science, 80: 156–160. DOI: 10.1093/ps/80.2.156
Stutz MW & Lawton GC. 1984. Effects of diet and antimicrobials on growth, feed efficiency, intestinal Clostridium perfringes, and ileal weight of broiler chicks. Poultry Science, 63: 2036-2042. DOI: 10.3382/ps.0632036
Talabi AO, Oyekunle MA, Adebayo LA & Apata SE. 2013. Effect of regimes of dietary oxytetracycline on the performance of broiler chicken. African Journal of Livestock Extension, 11: 26-30.
Tannock GW. 1998. The normal microflora: new concepts in health promotion. Microbiological Science, 5: 4-8.
Toghyani M, Toghyani M & Tabeidian SA. 2011. Effect of probiotic and prebiotic as antibiotic growth promoter substitutions on productive and carcass traits of broiler chicks.
2011 International Conference on Food Engineering and Biotechnology (IPCBEE), 9: 82-86.
Vassiliadis P, Trichopoulos D, Kalandidi A & Xirouchaki E. 1978. Isolation of Salmonellae from sewage with a new procedure of enrichment. Journal of Applied Microbiology, 44: 233-239. DOI: 10.1111/j.1365-2672.1978.tb00795.x
Zulkifli I, Abdullah N, Azrin NM & Ho YM. 2000. Growth performance and immune response of two commercial broiler strains fed diets containing Lactobacillus cultures and oxytetracycline under heat stress conditions. British Poultry Science, 41: 593-597. DOI: 10.1080/713654979
Poultry Science Journal
Volume 5, Issue 1
June 2017
Pages 41-50

Files

Share

How to cite

Statistics