The Impact of in ovo Injection of Silver Nanoparticles, Thyme and Savory Extracts in Broiler Breeder eggs on Growth Performance, Lymphoid-Organ Weights, and Blood and Immune Parameters of Broiler Chicks

Document Type : Original Paper


Department of Animal Science, Agriculture Faculty, Bu – Ali Sina University, Hamedan, Iran.


This study was conducted to evaluate the long-term effects of in ovo injection of nano-silver, thyme and savory extracts in broiler breeder eggs on growth performance, lymphoid organ weights, immune and blood parameters and bone mineral concentration of broiler chicks. A total of 560 fertilized broiler breeder eggs were divided into 7 groups. On d 5 of incubation, eggs were treated with the following groups. Group 1 (control, no injection); group 2 (placebo, 1 mL of 0.9% NaCl); group 3 and 4 (placebo + 30 and 45 mg of nano-silver, respectively); group 5 and 6 (placebo + 75 and 100 mg of thyme, respectively) and group 7 (placebo + 75 mg of savory). After hatch, chickens were fed a corn-soybean meal diet under the controlled conditions and slaughtered at 14 and 21 d of age for sample collection and analysis. The lymphoid-organ weights and growth rate were not affected by dietary treatments at 14 and 21 d of age. The results also showed that nano-silver injected into broiler breeder eggs during incubation improved the bone mineral concentration and cell-mediated immunity at 14 and 21 d of age, respectively. Humoral immunity was improved by thyme and savory extracts (P < 0.05). Overall, the effect of in ovo injection of nano-silver, thyme and savory extracts during embryonic development is a potential means to improve immune activities of broiler chickens, while does not have any detrimental effect on embryo hatchability.


 Ahmadi F & Hafsi Kurdestany A. 2010. The impact of silver nano particles on growth performance, lymphoid organs and oxidative stress indicators in broiler chicks. Global Veterinaria, 5: 366- 370. [Link]
Ahmadi F & Hafsy Kordestany A. 2011. Investigation on silver retention in different organs and oxidative stress enzymes in male broiler fed diet supplemented with powder of nano silver. American-Eurasian Journal of Toxicological Sciences, 3: 28-35.[Link]
Anonymous. 2015. /wiki/Cell-mediated_immunity.
AOAC (Association of Official Analytical Chemists). 1984. Official Methods of Analysis. 14th Ed. Washington DC, USA. [Link]
AOAC (Association of Official Analytical Chemists). 1990. Official Methods of Analysis. 15th Ed. Washington DC, USA. [Link]
Bhol KC & Schechter PJ. 2005. Topical nanocrystalline silver cream suppresses inflammatory cytokines and induces apoptosis of inflammatory cells in a murine model of allergic contact dermatitis. British Journal of Dermatology, 152: 1235-1242. [Link]
Binion R. 2008. Nanotechnology: A little bit like asbestos? Mealey’s Litigation Report: Insurance 22, no 28: 1-5. [Link]
Corrier DE & Deloach JR. 1990. Evaluation of cell-mediated cutaneous basophil hypersensitivity in young chickens by an interdigital skin test. Poultry Science, 69: 403-408. [Link]
Dibner JJ, Richards JD, Kitchell ML & Quiroz MA. 2007. Metabolic challenges and early bone development. Journal of Applied Poultry Research, 16: 126-137. [Link]
Elwinger K, Berndtson E, Engström B & Waldenstedt L. 1998. Effect of antibiotic growth promoters and anticoccidials on growth of Clostridium perfringens in the caeca and on performance of broiler chickens. Acta Veterinaria Scandinavica, 39: 433-441. [Link]
Ghalamkari G, Toghyani M, Tavalaeian E,  Landy N, Ghalamkari Z & Radnezhad H. 2011. Efficiency of different levels of Satureja hortensis L. (Savory) in comparison with an antibiotic growth promoter on performance, carcass traits, immune responses and serum biochemical parameters in broiler chickens. African Journal of Biotechnology, 10: 13318-13323. [Link]
Grodzik M & Sawosz E. 2006. The influence of silver nanoparticles on chicken embryo development and bursa of Fabricius morphology.  Journal of Animal and Feed Sciences, 15: 111-114. [Link]
Güllüce M, Sökmen M, Daferera D, Agar G, Özkan H, Kartal N, Polissiou M, Sökmen A & Sahin F. 2003. In vitro antibacterial, antifungal, and antioxidant activities of the essential oil and methanol extracts of herbal parts and callus cultures of Satureja hortensis L. Journal of Agricultural and Food Chemistry, 51: 3958 -3965. [Link]
Hosseini Mansoub N & Mohammad Nezhady AM. 2011. The effect of using Thyme, Garlic and Nettle on performance, carcass quality and blood parameters. Annals of Biological Research, 2: 315-320. [Link]
Kim YS, Kim JS, Cho HS, Rha DS, Kim JM, Park JD, Choi BS, Lim R, Chang HK, Chung YH, Kwon IH, Jeong J, Han BS & Yu IJ. 2008. Twenty-eight-day oral toxicity, genotoxicity, and gender-related tissue distribution of silver nanoparticles in Sprague-Dawley rats. Inhalation Toxicology, 20: 575-583. [Link]
Matsumura Y, Yoshikata K, Kunisaki S & Tsuchido T. 2003. Mode of bactericidal action of silver zeolite and its comparison with that of silver nitrate. Applied and Environmental Microbiology, 69: 4278-4281. [Link]
Mihajilov-Krstev T, Radnovic D, Kitic D, Stojanovic-Radic Z & Zlatkovic B. 2009. Antimicrobial activity of Satureja hortensis L. essential oil against pathogenic microbial strains. Biotechnology and Biotechnological Equipment, 23: 1492-1496. [Link]
Mihajilov-Krstev T, Radnovic D, Kitic D, Stojanovic-Radic Z & Zlatkovic B. 2010. Antimicrobial activity of Satureja hortensis L. essential oil against pathogenic microbial strains. Archives of Biological Science Belgrade. 62:159-166. [Link]
Paleo AJ, Martinez-Boubeta C, Balcells L, Costa CM, Sencadas V & Lanceros-Mendez S. 2011. Thermal, dielectrical and mechanical response of α and β-poly (vinilydene fluoride)/Co-MgO nanocomposites. Nanoscale Research Letters, 6: 257-260. [Link]
SAS (Statistical Analysis System). 2003. SAS/STAT® 9.1. User's Guide. SAS Institute Inc. Cary, North Carolina. [Link]
Sawosz E, Grodzik M, Zielin´ska M, Niemiec T, Olszan´ska B & Chwalibog A. 2009. Nanoparticles of silver do not affect growth, development and DNA oxidative damage in chicken embryos. Archiv für Geflügelkunde, 73: 208-213. [Link]
Studnicka A, Sawosz E, Grodzik M, Balcerak M & Chwalibog A. 2009. Influence of nanoparticles of silver/palladium alloy on chicken embryos’ development. Animal Science, 46: 237-242. [Link
Tampieri MP, Galuppi R, Macchioni F, Carelle MS, Falcioni L, Cioni PL & Morelli I. 2005. The inhibition of Candida albicans by selected essential oils and their major components. Mycopathologia, 159: 339- 345. [Link]
Varel VH. 2002. Carvacrol and thymol reduce swine waste odour and pathogens: stability of oils. Current Microibology, 44: 38-43. [Link]
Verma J, Johri TS, Swain BK & Ameena S. 2004. Effect of graded levels of aflatoxin, ochratoxin and their combinations on the performance and immune response of broilers. British Poultry Science, 45: 512-518. [Link]
Wennberg C, Hessle L,  Lundberg P, Mauro S, Narisawa S, Lerner UH & Millán JL. 2000. Functional characterization of osteoblasts and osteoclasts from alkaline phosphatase knockout Mice. Journal of Bone and Mineral Research, 15: 1879-1888. [Link]
Wijnhoven SWP, Peijnenburg WJGM,  Herberts CA,  Hagens WI,  Oomen AG,  Heugens EHW,  Roszek B,  Bisschops J,  Gosens I,  Meent DVD,  Dekkers S,  De Jong WH,  Zijverden MV,  Sips AJAM & Geertsma RE. 2009. Nano-silver - a review of available data and knowledge gaps in human and environmental risk assessment. Nanotoxicology, 3: 109-138. [Link]
Yin HQ, Langford R & Burrell RE. 1999. Comparative evaluation of the antimicrobial activity of ACTICOAT antimicrobial barrier dressing. Journal of Burn Care and Research, 20: 195-200. [Link]
Zamani Moghaddam AK, Ghanadi AR, Gafarian A & Shojadoost B. 2007. The effect of Satureja hortensis on performance of broiler chickens and NDHI titers. 16th European symposium on poultry nutrition, Strasburg, France. [Link]
Zhang H, Kong B, Xiong YL & Sun X. 2009. Antimicrobial activity of spice extracts against pathogenic and spoilage bacteria in modified atmosphere packaged fresh pork and vacuum packaged ham slices stored at 4ºC. Meat Science, 81: 686-692. [Link]