Growth Performance, Carcass Characteristics, Antibody Titer and Blood Parameters in Broiler Chickens Fed Dietary Myrtle (Myrtus communis) Essential Oil as an Alternative to Antibiotic Growth Promoter

Document Type : Original Paper

Authors

Department of Animal and Poultry Sciences, University of Tehran, Aburaihan Campus, Pakdasht, Tehran, Iran.

Abstract

This experiment was conducted to determine the effects of Myrtle Essential Oil (MEO) on growth performance, carcass characteristics, antibody titer and blood parameters of broiler chickens. A total of 200 Ross 308 broiler chickens were allocated to five dietary treatments with four replicates of 10 birds each. Dietary treatments were prepared by formulating a corn-soybean meal-based diet free of antibiotics (Control) and supplementing the basal diet with three levels of MEO at 100, 200, 300 mg/Kg or antibiotic Flavophospholipol (FPL) at 600 mg/Kg. The results showed that diets supplemented with MEO and FPL increased the feed intake, body weight gain and improved the feed conversion ratio compared to the control treatment (P). The relative carcass weight was significantly increased, whereas the weight of gastrointestinal tract and liver were decreased in broilers fed MEO (P). Supplementing the basal diet with MEO increased the antibody titers against Avian Influenza Virus (AIV) and Newcastle disease Virus (NDV), although supplementing diet with 200 mg/Kg of MEO was more effective (P). Broilers fed MEO diets especially at the level of 300 mg/Kg had a lower white blood cells count and heterophil, heterophil to lymphocyte ratio, mean corpuscular volume and mean corpuscular hemoglobin, but a higher lymphocyte and red blood cells count (P). In conclusion, data showed that diet supplemented with MEO improved the growth performance and increased antibody titers against AIV and NDV, especially at the level of 200 mg/Kg, in broiler chickens and could be an adequate alternative to antibiotics.

Keywords

References

Akin M, Aktumsek A & Nostro A. 2010. Antibacterial activity and composition of the essential oils of Eucalyptus camaldulensis Dehn and Myrtus communis L. growing in Nothern Cyprus. African Journal of Biotechnology, 9: 531-535.
Al-Ankari AS, Zaki MM & Al-Sultan SI. 2004. Use of habek mint (Mentha longifolia) in broiler chicken diets. International Journal of Poultry Science, 3: 629-634.
Alçiçek A, Bozkurt M & Çabuk M. 2003. The effect of an essential oil combination derived from selected herbs growing wild in Turkey on broiler performance. South African Journal of Animal Science, 33: 89-94.
Al‐Hindawi MK, Al‐Deen IH, Nabi MH & Ismail MA. 1989. Anti-inflammatory activity of some Iraqi plants using intact rats. Journal of Ethnopharmacology, 26: 163‐168.
Al-Kassie GAM. 2008. The effect of anise and rosemary on broiler performance. International Journal of Poultry Science, 7: 243-245.
Ashraf s, Zaneb H, Yousef MS, Ijaz A, Sohail MU, Muti S, Usman MM, Ijaz S & Rehman H. 2013. Effect of dietary supplementation of prebiotics and probiotics on intestinal microarchitecture in broilers reared under cyclic heat stress. Journal of Animal Physiology and Animal Nutrition, 1: 68-73.
Avigen. 2009. Ross 308 broiler manual. http://en.aviagen.com/ross-308/.
Baytop T. 1999. Therapy with medicinal plants in Turkey (Past and Present). Istanbul University Publications. No. 3255/40. 371 Pages.
Biricik H, Yesilbag D, Gezen SS & Bulbul T. 2012. Effects of dietary myrtle oil (Myrtus communis L.) supplementation on growth performance, meat oxidative stability, meat quality and erythrocyte parameters in quails. Revue De Medecine Veterinaire, 163: 131-138.
Botsoglou NA, Christaki E, Florou-Paneri P, Giannenas I, Papageorgiou G & Spais AB. 2004. The effect of a mixture of herbal essential oils or tocopheryl acetate on performance parameters and oxidation of body lipid in broilers. South African Journal of Animal Science, 34: 52-61.
Brugh M, Beard CW & Wilkes WJ. 1978. The influence of test conditions on Newcastle disease hemagglutination-inhibition titers. Avian Disease, 22: 320-328.
Campbell TW. 1995. Avian hematology and cytology. Iowa State University Press. USA. 104 Pages.
Cross DE, McDevitt RM, Hillman K & Acamovic T. 2007. The effect of herbs and their associated essential oils on performance, dietary digestibility and gut microflora in chickens from 7 to 28 days of age. British Poultry Science, 48: 496-506.
Di Pasqua R, Betts G, Hoskins N, Edwards M, Ercolini D & Mauriello G. 2007. Membrane toxicity of antimicrobial compounds from essential oils. Journal of Agricultural  and Food Chemistry, 55: 4863–4870.
Gardeli C, Vassiliki P, Athanasios M, Kibouris T & Komaitis M. 2008.  Essential oil composition of Pista cialentiscus L. and Myrtus communis L.: Evaluation of antioxidant capacity of methanolic extracts. Food Chemistry, 107: 1120-1130.
Gollnisch K, Wald C & Berk A. 2001. The use of different essential oils in the breeding of piglets. 6th German Society Quality Research Conference. Jena, Germany. Pages, 259–262.
Gross WB & Siegel HS. 1983. Evaluation of heterophil/lymphocyte ratio as a measure of stress in chickens. Avian Disease, 27: 972–979.‎
Hayder N, Kilani S, Abdelwahed A, Mahmoud A, Meftahi K, Ben Chibani J, Ghedira K & Chekir-Ghedira L. 2003. Antimutagenic activity of aqueous extracts and essential oil isolated from Myrtus communis. Die Pharmazie-An International Journal of Pharmaceutical Sciences, 58: 523–524.
Hernandez F, Madrir J, Garcia V, Orengo J & Megias MD. 2004. Influence of two plant extracts on broiler performance, digestibility and digestive organ size. Poultry Science, 83: 169-174.‎
Jang IS, Ko YH, Kang SY & Lee CY. 2007. Effect of commercial essential oil on growth performance, digestive enzyme activity and intestinal microflora population in broiler chickens. Animal Feed Science and Technology, 134: 304-315.
Kececi T, Oguz H, Kurtoglu V & Demet O. 1998. Effects of polyvinylpolypyrrolidone, synthetic zeolite and bentonite on serum biochemical and haematological characters of broiler chickens during aflatoxicosis. British Poultry Science, 39: 452–458.
Lee KW, Everts H, Kappert HJ, Frehener M, Losa R & Beynen AC. 2003. Effects of dietary essential oil component on growth performance, digestive enzymes and lipid metabolism in female broiler chickens. British Poultry Science, 44: 450-457.
Lee KW, Everts H, Kappert HJ & Breynen AC. 2004. Growth performance of broiler chickens fed a carboxymethyl cellulose containing diet with supplemental carvacrol and/or cinnamaldehyde. International Journal of Poultry Science, 3: 619–622.
Liu XY. 1999. Stress and immunity. In: Yin TB (Eds). Poultry immunology. China Agriculture Press. Beijing, China. Pages, 230–252.
Madrid J, Hernandez F, Garcia V, Orengo J, Megias MD & Savilla V. 2003. Effect of plant extracts on ileal apparent digestibility and carcass yield in broilers at level of farm. 14th European Symposium of Poultry Nutrition. Lillehammer, Norway. Pages, 187-188.
Martin T, Rubio B, Villaescua L, Fernandez L & Diaz AM. 1999. Polyphenolic compounds from pericarps of Myrtus communis. Pharmaceutical Biology, 37: 28-31.
Najafi P & Torki M. 2010. Performance, blood metabolites and immuno-competence of broiler chicks fed diets included essential oils of medicinal herbs. Journal of Animal and Veterinary Advances, 9: 1164-1168.
Neu HC. 1992. The crisis in antibiotic resistance. Science, 257: 1064–1073.
Ozek T, Demirci B & Baser KHC. 2000. Chemical composition of Turkish myrtle oil. Journal of Essential Oil Research, 12: 541-544.
Rahimi S, Teymouri Zadeh Z, Karimi Torshizi MA, Omidbaigiand R & Rokni H. 2011. Effect of the three herbal extracts on growth performance, immune system, blood factors and intestinal selected bacterial population in broiler chickens. Journal of Agriculture Science Technology, 13: 527-539.
Rasooli I, Moosavi ML, Rezaee MB & Jaimand K. 2002. Susceptibility of microorganisms to Myrtus communis L. essential oil and its chemical composition. Journal of Agricultural Science and Technology, 4: 127-133.
Ricke SC. 2003. Perspectives on the use of organic acids and short chain fatty acids as antimicrobials. Poultry Science, 82: 632–639.
Romani A, Coinu R, Carta S, Pinelli P,  Galardi C, Vincieri FF & Franconi F. 2004. Evaluation of antioxidant effect of different extracts of Myrtus communis L. Free Radical Research, 38: 97‐103.
SAS (Statistical Analysis System). 2001. SAS/STAT® 8.0. User's guide. SAS Institute Inc. Cary, NC.
Sohail MU, Hume ME, Byrd JA, Nisbet DJ, Ijaz A, Sohail A, Shabbir MZ & Rehman H. 2012. Effect of supplementation of prebiotic mannan-oligosaccharides and probiotic mixture on growth performance of broilers subjected to chronic heat stress. Poultry Science, 91: 2235-2240.
Thakar M. 2004. Pharmacological screening of some medicinal plants as antimicrobial and feed additives. Msc Dissertation, Virginia Polytechnic Institute and State University. Virgina, USA. 73 Pages.
Toghyani M, Gheisari A, Ghalamkari G & Mohammadrezaei M. 2010. Growth performance, serum biochemistry and blood hematology of broiler chicks fed different levels of black seed (Nigella sativa) and peppermint (Mentha piperita). Livestock Science, 129: 173-178.
Yadegarinia D, Gachkar L, Rezaei MB, Taghizadeh M, Astaneh SA & Rasooli I. 2006. Biochemical activities of Iranian Mentha piperita L. and Myrtus communis L. essential oils. Phytochemistry, 67: 1249‐1255.
Please cite this article as: Mahmoodi Bardzardi M, Ghazanfari S, Salehi A & Sharifi SD. 2014. Growth performance, carcass characteristics, antibody titers and blood parameters in broiler chickens fed dietary myrtle (Myrtus communis) essential oil as an alternative to antibiotic growth promoter. Poult. Sci. J. 2 (1): 37-49.
Poultry Science Journal
Volume 2, Issue 1 - Serial Number 1
January 2014
Pages 37-49

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