Candidate Gene Polymorphism for IL-Rγ and ChB6 Genes in the Indigenous Chicken of North Western Himalayan State of Himachal Pradesh, India

Document Type : Original Paper

Authors

Animal Genetics & Breeding, DGCN College of Veterinary & Animal Sciences CSKHPKV, Palampur, India -176062.

Abstract

Present investigation was carried out to identify DNA polymorphism of IL-2Rγ and ChB6 genes. Sixty five birds belonging to the indigenous chicken of Himachal Pradesh were utilized. Good quality DNA samples were subjected to PCR-RFLP analysis using chicken specific primers. Overnight restriction enzyme digestion was carried out at 37°C with IU Hph I and Pvu II for IL-2Rγ and ChB6 genes, respectively. Amplification of IL-2Rγ gene resolved a 600 bp amplicon in all samples,  which upon digestion with Hph I RE yielded three patterns i.e. Hph I aa , Hph I a/b and Hph I bb. Hph I aa genotype revealed 465 and 42 bp fragments while Hph I bb genotypes revealed 454, 134, 104 and 42 bp fragments. Hph I a/b genotype resolved 465, 454, 134, 104 and 42 bp fragments. The frequencies of these patterns were 0.47, 0.23 and 0.30 for Hph I aa, Hph I a/b and Hph I bb, respectively. The amplification of Chicken B cell marker (ChB6) generated 215 bp amplicon in all the samples, which upon digestion with  Pvu II generated two pattern i.e. Pvu II aa and Pvu II bb respectively. Pvu II aa generated 215 bp fragments while Pvu II bb generated 215, 147 and 68 bp, respectively. The frequencies of the PCR-RFLP pattern of ChB6 gene were 0.57 and 0.43, respectively. 

Keywords

References

Gilmour DG, Collins WM, Fredericksen TL, Jr.Urban WE, Ward PF & DiFronzo NL. 1986. Genetic interaction between non-MHC T- and B-cell alloantigens in response to Rous sarcomas in chickens. Immunogenetics, 23:1-6.
Jaiswal G, Kumar S, Prasad Y & Singh DP. 2009. PCR-RFLP analysis of IL-2Rγ and IL-15Rα genes in kadakanath native chicken. Journal of Applied Animal Research, 36: 239-242.
Kagami H, Nakamura H & Tomita T. 1990. Sex identification in chickens by means of the presence of the W chromo-some-specific repetitive DNA  units. The Journal of Poultry Science, 27: 379-384.
Katoch S, Kaila OP, Sankhyan V, Mahajan K, Shekhar C, Verma N, Sarma LM & Reen JK. 2010. Rural poultry production for sustainable livelihood in Himachal Pradesh. Proceedings in National Seminar on Conventional and Modern Breeding Technologies for Genetic Improvement of Livestock and Poultry. Pantnagar, India.
Singh AP, Kumar S & Gupta T. 2008. PCR RFLP analysis of IL-2Rγ and 11-15Rα genes in specialized layer chicken lines. Journal of Applied Animal Research, 33: 29-31.
Tregaskes CA, Bumstead N, Davison TF & Young JR. 1996. Chicken B-cell marker ChB6 (Bu-1) is a highly glycosylated protein of unique structure. Immunogenetics, 44:212-217.
Zhou H & Lamont SJ. 2003. Association of six candidate genes with antibody response kinetics in hens. Poultry Science, 82: 1118-1126.
Please cite this article as: Reen JK, Sankhyan V, Katoch S & Thakur YP. 2013. Candidate gene polymorphism for IL-2R γ and ChB6 genes in the indigenous chicken of North Western Himalayan State of Himachal Pradesh, India. Poult. Sci. J. 1 (2): 87-92.

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