Effects of Dietary Mannan-Oligosaccharides and Phytase Supplementation Alone or in Combination on Growth Performance, Serum Metabolites, Cecal Microbiota Activity and Intestinal Morphology in Broiler Chickens

Document Type : Original Paper


Department of Animal Science, Qaemshahr Branch, Islamic Azad University, Qaemshahr, Iran


This study evaluated the effect of a combination of mannan-oligosaccharides (MOS) and microbial phytase (MP) on growth performance, some blood biochemical metabolites, intestinal morphology and cecal microbial population of ROSS 308 broiler chickens from d 0 to 35 of age. A total of 200 one-dayold broiler chickens (mixed sex) were randomly distributed into four treatments with five replicates and 10 birds per each. The dietary treatments included a basal diet, the basal diet supplemented with MOS (as 2 g/kg of diet), MP (as 100 g/t Phyzyme XP 5000), and the combination of MOS and MP (MOS+MP). Supplementation of MOS+MP improved feed conversion ratio and body weight gain of broilers compared with the control group (P < 0.05). Dietary treatments had no significant effect on carcass traits. In the intestinal morphometric indices, villus length was greater in the birds which received MP individually or in combination with MOS (P < 0.05). Supplementation of MP, MOS or MOS+MP increased serum concentration of calcium in the broilers compared with control treatment (P < 0.05). Broiler chickens fed diets containing MOS+MP had lower numbers of E. coli, but higher count of Lactobacilli rather than control group (P < 0.05). Based on these results, it is concluded that dietary supplementation of MP in combination with MOS improved growth performance and intestinal microbial ecosystem of broiler chickens.


Bogucka J, Dankowiakowska A, Elminowska-Wenda G, Sobolewska A, Szczerba A, &Bednarczyk M. 2016. Effects of prebiotics and synbiotics delivered in ovo on broiler small intestine histomorphology during the first days after hatching. Folia Biologica, 64: 131-143.  DOI: 10.3409/fb64_3.131
Cowieson A, Bedford M, Selle P, &Ravindran V. 2009. Phytate and microbial phytase: implications for endogenous nitrogen losses and nutrient availability. World's Poultry Science Journal, 65: 401-418. DOI: 10.1017/S0043933909000294
Dilger RN, Onyango E, Sands J, & Adeola O. 2004. Evaluation of microbial phytase in broiler diets. Poultry Science, 83: 962-970. DOI: 10.1093/ps/83.6.962
Eftekhari A, Rezaeipour V, &Abdullahpour R. 2015. Effects of acidified drinking water on performance, carcass, immune response, jejunum morphology, and microbiota activity of broiler chickens fed diets containing graded levels of threonine. Livestock Science, 180: 158-163. DOI: 10.1016/j.livsci.2015.07.010
Ghasemian M, &Jahanian R. 2016. Dietary mannan-oligosaccharides supplementation could affect performance, immunocompetence, serum lipid metabolites, intestinal bacterial populations, and ileal nutrient digestibility in aged laying hens. Animal Feed Science and Technololgy, 213: 81-89. DOI:10.1016/j.anifeedsci.2015.12.012
Gibson GR, &Roberfroid MB. 1995. Dietary modulation of the human colonic microbiota: introducing the concept of prebiotics. Journal of Nutrition, 125: 1401. DOI: 10.1093/jn/125.6.1401
Hajiaghapour M, &Rezaeipour V. 2018. Comparison of two herbal essential oils, probiotic, and mannan-oligosaccharides on egg production, hatchability, serum metabolites, intestinal morphology, and microbiota activity of quail breeders. Livestock Science, 210: 93-98. DOI: 10.1016/j.livsci.2018.02.007
Huang Q, Wei Y, Lv Y, Wang Y, &Hu T. 2015. Effect of dietary inulin supplements on growth performance and intestinal immunological parameters of broiler chickens. Livestock Science, 180: 172-176. DOI: 10.1016/j.livsci.2015.07.015
Huyghebaert G, Ducatelle R, &Van Immerseel F. 2011. An update on alternatives to antimicrobial growth promoters for broilers. Veterinary Journal, 187: 182-188. DOI: 10.1016/j.tvjl.2010.03.003
Jiang XR, Luo FH, Qu MR, Bontempo V, Wu SG, Zhang HJ, Yue HY, & Qi GH. 2013. Effects of non-phytate phosphorus levels and phytase sources on growth performance, serum biochemical and tibia parameters of broiler chickens. Italian Journal of Animal Science, 2: e60. DOI: 10.4081/ijas.2013.e60
Momeneh T, Karimi A, Sadeghi G, Vaziry A, &Bedford, M. 2018. Evaluation of dietary calcium level and source and phytase on growth performance, serum metabolites, and ileum mineral contents in broiler chicks fed adequate phosphorus diets from one to 28 days of age. Poultry Science, 97: 1283-1289. DOI: 10.3382/ps/pex432
Mourão JL, Pinheiro V, Alves A, Guedes C, Pinto L, Saavedra MJ, Spring P, &Kocher A. 2006. Effect of mannan oligosaccharides on the performance, intestinal morphology and cecal fermentation of fattening rabbits. Animal Feed Science and Technology, 126: 107-120. DOI: 10.1016/j.anifeedsci.2005.06.009
Narasimha J, Nagalakshmi D, Ramana Reddy Y, &Viroji Rao S. 2013. Synergistic effect of non starch polysaccharide enzymes, synbiotics and phytase on performance, nutrient utilization and gut health in broilers fed with sub-optimal energy diets. Veterinary World, 6. DOI: 10.14202/vetworld.2013.754-760
Pourabedin M, Xu Z, Baurhoo B, Chevaux E, &Zhao X. 2014. Effects of mannan oligosaccharide and virginiamycin on the cecal microbial community and intestinal morphology of chickens raised under suboptimal conditions. Canadian Journal of Microbiology, 60: 255-266. DOI: 10.1139/cjm-2013-0899
Rezaeipour V, Barsalani A, &Abdullahpour R. 2016. Effects of phytase supplementation on growth performance, jejunum morphology, liver health, and serum metabolites of Japanese quails fed sesame (Sesamum indicum) meal-based diets containing graded levels of protein. Tropical Animal Health and Production, 48: 1141-1146. DOI: 10.1007/s11250-016-1066-x
Roofchaei A, Rezaeipour V, Vatandour S, &Zaefarian F. 2019. Influence of dietary carbohydrases, individually or in combination with phytase or an acidifier, on performance, gut morphology and microbial population in broiler chickens fed a wheat-based diet. Animal Nutrition, 5: 63-67. DOI: 10.1016/j.aninu.2017.12.001
SAS. 2001. SAS Statistics User’s Guide. Statistical Analytical System, fifth revised ed. SAS Institute Inc, Carry, NC.
Selle PH, Cowieson AJ, &Ravindran V. 2009. Consequences of calcium interactions with phytate and phytase for poultry and pigs. Livestock Science, 124: 126-141. DOI: 10.1016/j.livsci.2009.01.006
Shang Y, Rogiewicz A, Patterson R, Slominski B, &Kim W. 2015. The effect of phytase and fructooligosaccharide supplementation on growth performance, bone quality, and phosphorus utilization in broiler chickens. Poultry Science, 94: 955-964.  DOI: 10.3382/ps/pev044
Sohail MU, Hume ME, Byrd JA, Nisbet DJ, Ijaz A, Sohail A,Shabbir MZ, &Rehman H. 2012. Effect of supplementation of prebiotic mannan-oligosaccharides and probiotic mixture on growth performance of broilers subjected to chronic heat stress. Poultry Science, 91: 2235-2240. DOI: 10.3382/ps.2012-02182
Sohail MU, Ijaz A, Younus M, Shabbir M, Kamran Z, Ahmad S, Anwar H,Yousaf MS, Ashraf K, Shahzad AH, &Rehman H. 2013. Effect of supplementation of mannan oligosaccharide and probiotic on growth performance, relative weights of viscera, and population of selected intestinal bacteria in cyclic heat-stressed broilers. Journal of Applied Poultry Research, 22: 485-491. DOI: 10.3382/japr.2012-00682
Teng PY, &Kim WK. 2018. Roles of prebiotics in intestinal ecosystem of broilers. Frontiers in Veterinary Science, 5. DOI: 10.3389/fvets.2018.00245
Viveros A, Brenes A, Arija I, &Centeno C. 2002. Effects of microbial phytase supplementation on mineral utilization and serum enzyme activities in broiler chicks fed different levels of phosphorus. Poultry Science, 81: 1172-1183. DOI: 10.1093/ps/81.8.1172
Wang Y, Zeng T, Wang S, Wang W, Wang Q, &Yu HX. 2010. Fructo-oligosaccharides enhance the mineral absorption and counteract the adverse effects of phytic acid in mice. Nutrition, 26: 305-311. DOI: 10.1016/j.nut.2009.04.014
Xu Z, Hu C, Xia M, Zhan X, &Wang M. 2003. Effects of dietary fructooligosaccharide on digestive enzyme activities, intestinal microflora and morphology of male broilers. Poultry Science, 82: 1030-1036. DOI:10.1093/ps/82.6.1030
Ziemer CJ, &Gibson GR. 1998. An overview of probiotics, prebiotics and synbiotics in the functional food concept: perspectives and future strategies. International Dairy Journal, 8: 473-479. DOI: 10.1016/S0958-6946(98)00071-5