The Effects of In ovoInjection of Ascorbic Acid on Hatchability, Growth Performance, Intestinal Morphology, and Tibia Breaking Strength in 36h Post Hatch Fasted Broiler Chickens

Document Type: Original Paper


1 Department of Animal Science, College of Agriculture, Shahid Bahonar University of Kerman, Kerman, Iran

2 Kerman Agricultural and Natural Resources Research and Education Center, Kerman, Iran


An experiment was conducted to evaluate the efficacy of intra-amnion administration of different doses of ascorbic acid (AA) on hatchability, growth performance, blood metabolites, jejunal morphology, and tibia breaking strength in 36h post hatch fasted broiler chickens. Two hundred eighty-eight Ross-308 fertile eggs at the 15thday of incubation were divided into four treatment groups, each containing four replicates of 18 eggs. The treatments included non-injected (control), injected into the amnion with 0.7 mL diluent (distilled water; sham treatment), and injected with 0.7 mL diluent containing 3 or 6 mg AA. The neonatal chicks were deprived of feed and water for 36 h. Hatched chicks were raised till 10 d of age. Hatch percentage was increased due to in ovo injection of 6 mg/egg AA.In ovo injection of different levels of AA had no significant effect on body weight gain, feed intake, and feed conversion ratio. In ovo injection of AA, especially at the 6 mg level, significantly increased villus height, villus width, and villus height: crypt depth ratio at 3 d of age. Tibia resistance and breaking strength were improved by in ovo injection of 6 mg AA when compared to the control at 10 d of age. In conclusion, it seems in ovo injection of 6 mg AA/egg on 15th d of incubation could have a positive impact on hatchability, intestinal morphology, and bone characteristics in broiler chickens.


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