The Effects of In ovoInjection of Ascorbic Acid on Hatchability, Growth Performance, Intestinal Morphology, and Tibia Breaking Strength in 36h Post Hatch Fasted Broiler Chickens

Document Type: Original Paper

Authors

1 Department of Animal Science, College of Agriculture, Shahid Bahonar University of Kerman, Kerman, Iran

2 Kerman Agricultural and Natural Resources Research and Education Center, Kerman, Iran

Abstract

An experiment was conducted to evaluate the efficacy of intra-amnion administration of different doses of ascorbic acid (AA) on hatchability, growth performance, blood metabolites, jejunal morphology, and tibia breaking strength in 36h post hatch fasted broiler chickens. Two hundred eighty-eight Ross-308 fertile eggs at the 15thday of incubation were divided into four treatment groups, each containing four replicates of 18 eggs. The treatments included non-injected (control), injected into the amnion with 0.7 mL diluent (distilled water; sham treatment), and injected with 0.7 mL diluent containing 3 or 6 mg AA. The neonatal chicks were deprived of feed and water for 36 h. Hatched chicks were raised till 10 d of age. Hatch percentage was increased due to in ovo injection of 6 mg/egg AA.In ovo injection of different levels of AA had no significant effect on body weight gain, feed intake, and feed conversion ratio. In ovo injection of AA, especially at the 6 mg level, significantly increased villus height, villus width, and villus height: crypt depth ratio at 3 d of age. Tibia resistance and breaking strength were improved by in ovo injection of 6 mg AA when compared to the control at 10 d of age. In conclusion, it seems in ovo injection of 6 mg AA/egg on 15th d of incubation could have a positive impact on hatchability, intestinal morphology, and bone characteristics in broiler chickens.

Keywords


Amiri N, Salarmoini M, Tasharofi S& Eslami M. 2015.  Investigation the effects of injection of different nutrients into yolk sac of fertile eggs, with 36 h post-hatch starvation, on hatchability, performance and blood metabolites of broilers. Iranian Journal of Animal Science, 46: 17-27 (in Persian).

AOAC. 2005. Official Methods of Analysis. AOAC International, Arlington, VA.

Bhanja AB, Mandal AB, Agarwal SK, Majumdar S&Bhattacharyya A. 2007. Effect of in ovo injection of vitamins on the chick weight and post-hatch growth performance in broiler chickens. Proc of the 16th European Symposium on Poultry Nutrition, Strasbourg, France.pp.143-146.

Bhattacharya L. 2010. Biochemistry of Nutrition. Firsted. Discovery Publishing Pvt. New Delhi, India.

Bohorquez DV, Bohorquez NE& Ferket PR. 2011. Ultrastructural development of the small intestinal mucosa in the embryo and turkey poult: A light and electron microscopy study. Poultry Science,90: 842-855. DOI: 10.3382/ps.2010-00939

Chamani M, Tasharofi S, Forudi F, Sadeghi AA& Aminafshar M. 2012. Evaluation the effects of in ovo injection of different nutrients on hatch percentage, performance and carcass parameters of broilers. Annals of Biological Research,3: 3771-3776.

Cheled-Shoval SL, Amit-Romach E, Barbakov M& Uni Z. 2011. The effect of in ovo administration of mannan oligosaccharide on small intestine development during the pre- and posthatch periods in chickens.  Poultry Science, 90: 2301-2310. DOI:10.3382/ps.2011-01488

Chou SH, Chung TK& Yu B. 2009. Effects of supplemental 25-hydroxycholecalciferol on growth performance, small intestinal morphology, and immune response of broiler chickens. Poultry Science,88: 2333-2341. DOI: 10.3382/ps.2009-00283

Decuypere E, Tona K, Bruggeman V& Bamelis F. 2001. The day-old chick, a crucial hinge between breeders and broilers. World’s Poultry Science Journal, 57: 127-138.  DOI: 10.1079/WPS20010010

Dorr PE& Nockels CF. 1971. Effects of aging and dietary ascorbic acid on tissue ascorbic acid in the domestic hen. Poultry Science,50: 1375-1382. DOI: 10.3382/ps.0501375

El-Gendi GM, Iraqi MM& Abdelrahman AA. 1999. Effect of vitamin C supplementation on some productive and physiological parameters in laying hens. Egyptian Journal of Nutrition and Feed,2: 649-664.

Eslami M, Salarmoini M& Tasharrofi S. 2014. Effects of in-ovo injection of different nutrients on the hatchability and growth performance in broilers. Journal of Livestock Science and Technologies,2: 1-7.

Foye OT, Uni Z& Ferket PR. 2006. Effect of in ovo feeding egg white protein, β-hydroxy-β-methylbutyrate, and carbohydrates on glycogen status and neonatal growth of turkeys. Poultry Science,85: 1185-1192. DOI: 10.1093/ps/85.7.1185

Frandson RD. 1986. Anatomy and Physiology of Farm Animals. 7th ed. Lea and Febiger Publisher, Phila, USA.

Geyra A, Uni Z& Sklan D. 2001. Enterocyte dynamics and mucosal development in the posthatch chick. Poultry Science,80:776-782. DOI: 10.1093/ps/80.6.776

Ghonim AI, Awad AL, Fattouh MH& El-Shhat AM. 2009. Comparative study of ascorbic acid treatment methods on hatchability traits and growth performance of ducklings. Egyptian Poultry Science Journal, 29: 1085-1099.

Hajati H, Hassanabadi A, golian A, Nassiri Moghaddam H&Nassiri MR. 2014. The effect of in ovo injection of grape seed extract and vitamin C on hatchability, antioxidant activity, yolk sac weight, performance and ileal microflora of broiler chickens. Research Opinions in Animal and Veterinary Sciences,4: 633-638.

Hajati H, Hassanabadi A, golian A, Nassiri Moghaddam H&Nassiri MR. 2015. The Effect of grape seed extract and vitamin C feed supplementation on some blood parameters and HSP70 gene expression of broiler chickens suffering from chronic heat stress. Italian Journal of Animal Science,14:1-9. DOI: 10.4081/ijas.2014.3273

 Hartono K, Reed S, Ankrah N, Glahn R& Tako E. 2015. Alterations in gut microflora populations and brush border functionality following intra-amniotic daidzein administration. RSC Advances, 5: 6407-6412. DOI: 10.1039/C4RA10962G

Ipek A, Sahin U& Yilmaz B. 2003. The effect of in ovo ascorbic acid and glucose injection in broiler breeder eggs on hatchability and chick weight. Arch Geflügelk, 68: 132- 135.

Karadas F, Surai PF& Sparks NHC. 2011. Changes in broiler chick tissue concentrations of lipid-soluble antioxidants immediately post-hatch. Comparative Biochemistry and Physiology,160: 68-71. DOI: 10.1016/j.cbpa.2011.05.006

Keralapurath MM, Corzo A, Pulikanti R, Zhai W& Peebles ED. 2010. Effects of in ovo injection of L-carnitine on hatchability and subsequent broiler performance and slaughter yield. Poultry Science,89: 1497-1501. DOI: 10.3382/ps.2009-00551

Keshavarz K. 1996. The effect of different levels of vitamin C and cholecalciferol with adequate or marginal levels of dietary calcium on performance and eggshell quality of laying hens. Poultry Science,75:1227-1235. DOI: 10.3382/ps.0751227

Kutlu HR. 2001. Influences of wet feeding and supplementation with ascorbic acid on performance and carcass composition of broiler chicks exposed to a high ambient temperature. Archives of Animal Nutrition,54: 127-139. DOI: 10.1080/17450390109381972

Leeson S& Summers JD. 2001. Scott's Nutrition of the Chicken. 4th ed. Nottingham University Press, England.

McKay LI& Cidlowski JA. 2003. Physiologic and pharmacologic effects of corticosteroids. Holland-Frei cancer medicine. 6th ed. Hamilton: BC Decker.

McDowell LR. 2000. Vitamins in Animal and Human Nutrition. 2nd ed. Iowa State University Press, Iowa, USA.

Mousavi S, Shivazad M, Chamani M, Sadeghi A& Lotfolahian H. 2009. Study of in ovo feeding as an early nutrition method in broiler chicken. Dynamic Agriculture, 5: 417-425.

Newman S& Leeson S. 1997. Skeletal integrity in layers at the completion of egg production. World’sPoultry ScienceJournal, 53: 265-277. DOI: 10.1079/WPS19970021

Nowaczewski S, Kontecka H& Krystianiak S. 2012. Effect of in ovo injection of vitamin C during incubation on hatchability of chickens and ducks. Folia Biologica, 60: 93-97.

Pardue SL& Thaxton JP. 1986. Ascorbic acid in poultry. A review. World’s Poultry Science Journal, 42: 107-123.DOI: 10.1079/WPS19860009

SAS Institute. 2005. SAS User’s Guide. Version 9.1 ed. SAS Institute, Cary, NC.

Selim SA, Gaafar KM &El-Ballal SS. 2012. Influence of in ovo administration with vitamin E and ascorbic acid on the performance of Muscovy ducks. Emirates Journal of Food and Agriculture, 24: 264-271.

Tako E, Ferket PR& Uni Z. 2004. Effects of in ovo feeding of carbohydrates and beta-hydroxy beta- methyl butyrate on the development of chicken intestine. World's Poultry Science Journal, 57: 2023-2028. DOI: 10.1093/ps/83.12.2023

Tako E& Glahn RP. 2012. Intra-amniotic administration and dietary inulin affect the iron status and intestinal functionality of iron-deficient broiler chickens. Poultry Science,91: 1361-70. DOI: 10.3382/ps.2011-01864

Thompson KL& Applegate TJ. 2006. Feed withdrawal alters small-intestinal morphology and mucus of broilers. Poultry Science,85: 1535-1540. DOI: 10.1093/ps/85.9.1535

Weiser H, Schlachter M, Probst HP& Kormann AW. 1990. The relevance of ascorbic acid for bone metabolism. Proc of the 2nd Symposium. Kartause,  Ittingen, Switzerland.

Yair R, Shahar R& Uni Z. 2015. In ovo feeding with minerals and vitamin D3 improves bone properties in hatchlings and mature broilers. Poultry Science,94: 2695-707. DOI: 10.3382/ps/pev252

Zakaria AH& Al-Anez MA. 1996. Effects of ascorbic acid and cooling during egg incubation on hatchability culling, mortality, and body weights of broilers chickens. Poultry Science,75: 1204-1209. DOI: 10.3382/ps.0751204

Zakaria AH. 2001. Effect of ascorbic acid treatment during egg incubation on the pre-and post-hatching development of broiler chickens. Damascus University Journal for the Agricultural Sciences, 17: 118-130.

Zhai W, Rowe DE& Peebles ED. 2011. Effects of commercial in ovo injection of carbohydrates on broiler embryogenesis. Poultry Science,90:1295-1301. DOI: 10.3382/ps.2010-01130.