The Effect of Dietary Supplementation of Prebiotic and Probiotic on Performance, Humoral Immunity Responses and Egg Hatchability in Broiler Breeders

Document Type: Original Paper

Authors

1 Center of Excellence in the Animal Sciences Department, Ferdowsi University of Mashhad, Mashhad, Iran.

2 Department of Animal Science, College of Animal Science and Fisheries, Sari Agricultural Sciences and Natural Resources University, Sari, Iran.

Abstract

In this experiment, the influence of prebiotic and probiotic supplementation in the broiler breeder diets on body weight, mortality, feed intake, egg production, hatchability and humoral immunity response was investigated. A total number of 13140 female and 1260 male breeders (Cobb 500) with 26 wks of age were allocated to three treatments with six replicates (800 birds each replicate). Breeders were fed control basal diet, basal diet supplemented with prebiotic (mannan oligosaccharide) or probiotic (Protexin®) for 17 weeks. Body weight, feed intake and egg production were measured weekly during 26-40 wks of age. The hatchability of eggs was recorded on weeks 38, 39, and 40. Antibody production was recorded after 8 wks of prebiotic and probiotic supplementation. Prebiotic supplementation did not affect feed intake, the percentages of egg production and settable eggs percents. Prebiotic increased egg hatchability and reduced the percentages of infertile eggs, as well as dead embryo-in-shells. Antibody titers against influenza and reovirus were higher in prebiotic fed group, but there were no significant differences among the other blood antibody titers. Probiotic had no significant effect on the considered parameters. In conclusion, findings of present study showed that prebiotic improved egg hatchability and humoral immunity of broiler breeders.

Keywords


Abd El-Samee LD, El-Wardany I, Nematallah GA & Abo-El-Azab OM. 2013. Effect of dietary organic zinc and prebiotic on productive performance and immune response of growing quails. Iranian Journal of Animal Science, 3: 761-767.

Abd El-Samee LD, El-Wardany I,  Ali NG & Abo-El-Azab OM. 2012. Egg quality, fertility and hatchability of laying quails fed diets supplemented with organic zinc, chromium yeast or mannan oligosaccharides. International Journal of Poultry Science, 11: 221-224.

Bach Knudsen KE. 2001. Development of antibiotic resistance and options to replace antimicrobials in animal diets. Proceedings of the Nutrition Society, 60: 291-299.

Bailey JS, Blankenship LC & Cox NA. 1991. Effect of fructo-oligosaccharides on Salmonella colonization of the chicken intestine. Poultry Science, 70: 2433–2438.

Baurhoo B, Letellier A, Zhao X & Ruiz-Feria CA. 2007a. Cecal populations of lactobacilli and bifidobacteria and Escherichia coli populations after in vivo Escherichia coli challenge in birds fed diets with purified lignin or mannan oligosaccharides. Poultry Science, 86: 2509–2516.

Baurhoo B, Phillip L & Ruiz-Feria CA. 2007b. Effects of purified lignin and mannan oligosaccharides on intestinal integrity and microbial populations in the ceca and litter of broiler chickens. Poultry Science, 86: 1070–1078.

Baurhoo B, Ferket PR & Zhao X. 2009. Effects of diets containing different concentrations of manna oligosaccharide or antibiotics on growth performance, intestinal development, cecal and litter microbial populations and carcass parameters of broilers. Poultry Science, 88: 2262–2272.

Berry WD & Lui P. 2000. Egg production, egg shell quality and bone parameters in broiler breeder hens receiving Bio-Mos and Eggshell 49. Poultry Science, 79: 124 (Abstract).

Blomberg L, Krivan HC, Cohen PS & Conway PL. 1993. Piglet ileal mucus protein and glycolipid (galactosylceramide) receptors specific for Escherichia coli K88 fimbriae. Infection and Immunity, 61: 2526–2531.

Bouhnik Y, Flourie B, Ouarne F, Riottot M, Bisetti N, Bornet F & Rambaud JC. 1994. Effects of prolonged ingestion of fructo-oligosaccharides on colonic bifidobacteria, fecal enzymes and bile acids in humans. Gastroenterology. 106: 598 (Abstract).

Cervantes H. 2006. Banning antibiotic growth promoters: Learning from the European experience. Poultry International, 45: 14-15.

Cobb-Vantress. 2013. Cobb 500 TM: Breeder management supplement. Cobb - Vantress, Siloam Springs. AR.

Duncan DB. 1955. Multiple range and multiple F tests. Biometrics, 11: 1-42.

Flickinger EA, Schreijen EM, Patil AR, Hussein HS, Grieshop CM, Merchen NR & Fahey GC Jr. 2003. Nutrient digestibilities, microbial populations, and protein catabolites as affected by fructan supplementation of dog diets. Journal of Animal Science, 81: 2008–2018.

Gibson GR & Roberfroid MB. 1995. Dietary modulation of the human colonic microbiota: Introducing the concept of prebiotics. Journal of Nutrition, 125: 1401–1412.

Gibson GR & Wang X. 1994. Inhibitory effects of bifidobacteria on other colonic bacteria. Journal of Applied Bacteriology, 77: 412–420.

Hajati H & Rezaei M. 2010. The application of prebiotics in poultry production. International Journal of Poultry Science, 9: 298-304.

Hajati H, Hassanabadi A & Afzali N. 2012. Effect of prebiotic (Bio-MOS) on broiler breeder performance and immunity system. 3rd International Veterinary Poultry Congress. Tehran, Iran. Page, 145.

Hofacre CL, Mathis GF & Quiroz MA. 2005. Natural alternatives to prevent necrotic enteritis. International Journal of Poultry Production, 13: 7–9.

International Animal Health, 1999. Protexin multi-strain probiotic. Cyberhorse. http://wwwcyberhorse. net.au/ian// protexin.htm.

Kidd MT, Peebles ET, Whitmarsh SK, Yeatman JB & Wideman RF. 2001. Growth and immunity of broiler chicks as affected by dietary arginine. Poultry Science, 80: 1535-1542.

Kim CH, Shin KS, Woo KC & Paik IK. 2009. Effect of dietary oligosaccharides on the performance, intestinal microflora and serum immunoglobulin contents in laying hens. Korean Journal of Poultry Science, 36: 125–131.

King'ori AM. 2011. Review of the factors that influence egg fertility and hatchability in poultry. International Journal of Poultry Science, 10: 483-492.

Marquardt WW, Synder DD, Savage PK, Kadavil, SK & Yancey FS. 1984. Antibody response to Newcastle disease virus given by two different routes as measured by ELISA and hemaglutination-inhibition test and associated tracheal immunity. Avian Disease, 29: 71-79.

McDaniel GR & Sefton T. 1991. Effect of yeast culture (Yea sacc 1026) supplementation on broiler breeders. Poultry Science, 70: 172 (Abstract).

McKay DM & Perdue MH. 1993. Intestinal epithelial function: The case for immune physiological regulation. Digestive Disease and Sciences, 38: 1377–1387.

Newman K. 1994. Mannan-oligosaccharides: Natural polymers with significant impact on the gastrointestinal microflora and the immune system. In: Lyons TP & Jacques KA. (Eds). Biotechnology in the feed industry. Nottingham University Press. Nottingham, UK. Pages, 167–174.

Nollet L. 2005. AGP alternatives-part I. EU close to a future without antibiotic growth promoters. World Poultry, 21: 14-15.

Oyofo BA, DeLoach JR, Corrier DE, Norman JO, Ziprin RL & Mollenhauer HH. 1989. Effect of carbohydrates on Salmonella typhimurium colonization in broiler chickens. Avian Disease, 33: 531–534.

Patterson HA & Burkholder KM. 2003. Application of prebiotic and prebiotics in poultry production. Poultry Science, 82: 627–631.

Riad S.A, Safaa HM, Fatma A, Mohamed Salwa SS & El-Minshawy HA. 2010. Influence of probiotic, prebiotic and/or yeast supplementation in broiler diets on the productivity, immune response and slaughter traits. Journal of Animal Poultry Production, 1: 45-60.

Russell MW, Reinholdt J & Kilian M. 1989. Anti-inflammatory activity of human IgA antibodies and their Fabα fragments: Inhibition of IgG-mediated complement activation. European Journal of Immunology, 19: 2243–2249.

Sadeghi AA, Mohammadi A, Shawrang P & Aminafshar M. 2013. Immune responses to dietary inclusion of prebiotic-based mannan-oligosaccharide and β-glucan in broiler chicks challenged with Salmonella enteritidis. Turkish Journal of Veterinary and Animal Science, 37: 206-213.

Salyers AA & Whitt DD. 2002. Bacterial Pathogenesis: A molecular approach. 2nd Ed. ASM Press. Washington, DC. 560 Pages.

SAS (Statistical Analysis System). 2000. SAS/STAT® 8.01. User's Guide. SAS Institute Inc. Cary, NC.

Shashidhara RG & Devegowda G. 2003. Effect of dietary mannan oligosaccharide on broiler breeder production traits and immunity. Poultry Science, 82: 1319–1325.

Sklan D, Melamed D & Friedman A. 1994. The effect of varying levels of dietary vitamin A on immune response in the chick. Poultry Science, 73: 843–847.

Spring P, Wenk C, Dawson KA & Newman KE. 2000. The effects of dietary mannan oligosaccharides on cecal parameters and the concentrations of enteric bacteria in the ceca of Salmonella challenged broiler chicks. Poultry Science, 79: 205–211.

Stanley VG, Brown C & Sefton T. 2000. Single and combined effects of dietary protease and mannan oligosaccharide on the performance of laying hens. Poultry Science, 79: 62 (Abstract).

Sultan KH & Abdul-Rhaman SY. 2011. Effect of probiotic on some physiological parameters in broiler breeders. International Journal of Poultry Science, 10: 626-628.

Thomas WE, Nilsson LM, Forero M, Sokurenko EV & Vogel V. 2004. Shear-dependent “stick-and-roll” adhesion of type Ι fimbriated Escherichia coli. Molecular Microbiology, 53: 1545–1557.

Vahdatpour T, Nikpiran H, Babazadeh D, Vahdatpourand S & Jafargholipour MA. 2011. Effects of Protexin®, Fermacto® and combination of them on blood enzymes and performance of Japanese quails (Coturnix Japonica). Annals of Biological Researches, 2: 283-291.

Wakeman GW. 2005. AGP alternatives- Part II. Dietary strategies to influence bacterial microflora. World Poultry, 21: 28-29.

Please cite this article as: Hajati H, Hassanabadi A & Teimouri Yansari A . 2014. The Effect of Dietary Supplementation of Prebiotic and Probiotic on Performance, Humoral Immunity Responses and Egg Hatchability in Broiler Breeders. Poult. Sci. J. 2 (1): 1-13..